Triterpenes Production by Rhizogenic Callus of Salvia Scabiosifolia Lam. Obtained via Agrobacterium Rhizogenes Mediated Genetic Transformation

ABSTRACT Rhizogenic callus of Salvia scabiosifolia Lam. formed after transformation with Agrobacterium rhizogenes ATCC 15834 was isolated and investigated for triterpenes production. The rhizogenic callus was cultivated on MS medium free of phytohormones and showed stable morphological and growth characteristic. The genetic transformation was confirmed by PCR amplification of rolA gene, found in the genome of transformed rhizogenic callus cells. Cell suspension was successfully initiated by transferring rhizogenic callus on LS medium supplied with 0.2 mg/L 2,4-D. The suspension culture was stable, fast growing (accumulated 1.709 g dry biomass/L) and produced 829.14 7mu;g/g dry biomass oleanolic acid. This is the first report for initiation of rhizogenic cell suspension culture from the rare plant Salvia scabiosifolia Lam.

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