Decreased Heparin Sensitivity of Cyclohexanedione-Modified Factor Xa

Summary Modification of 12 arginine residues of bovine Factor Xa with 1,2-cyclohexanedione has resulted in a decrease in heparin sensitivity of the reaction between enzyme and antithrombin-III, whereas the antithrombin-III sensitivity of modified Factor Xa was only slightly affected. It is suggested that heparin accelerates the Factor Xa-antithrombin-III reaction by interacting with Factor Xa.

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