An experimental setup for traceable measurement and calibration of liquid flow rates down to 5 nl/min

Abstract This work presents the improvements of an experimental setup for measuring ultra-low flow rates down to 5 nl/min. The system uses a telecentric CCD imaging system mounted on a high-precision, computer-controlled linear stage to track a moving liquid meniscus inside a glass capillary. Compared to the original setup, the lowest attainable expanded uncertainty at any flow rate has been reduced from 5.4% to 2%. In addition, the conformity with specification of three commercial micro-fluidic devices was evaluated using the new setup: one syringe pump, one implantable infusion pump and one thermal flow sensor. The flow sensor and the implantable infusion pump met the compliance criteria (coverage probability 95%). The syringe pump however, failed to meet the specifications at 5 nl/min and 10 nl/min. No assessment could be made at higher flow rates.

[1]  O. Mayboroda,et al.  Ultra-low flow electrospray ionization-mass spectrometry for improved ionization efficiency in phosphoproteomics. , 2012, Analytical chemistry.

[2]  Eduard Gröller,et al.  Surface Extraction from Multi-Material Components for Metrology using Dual Energy CT , 2007, IEEE Transactions on Visualization and Computer Graphics.

[3]  James T. Webber,et al.  Nanoflow low pressure high peak capacity single dimension LC-MS/MS platform for high-throughput, in-depth analysis of mammalian proteomes. , 2012, Analytical chemistry.

[4]  Javier Santillan,et al.  Precise 3D dimensional metrology using high-resolution x-ray computed tomography (μCT) , 2010, Optical Engineering + Applications.

[5]  T. Wirth.  Microreactors in organic chemistry and catalysis , 2013 .

[6]  G. D. de Jong,et al.  Low-flow sheathless capillary electrophoresis-mass spectrometry for sensitive glycoform profiling of intact pharmaceutical proteins. , 2013, Analytical chemistry.

[7]  Mani B. Srivastava,et al.  Exploiting manufacturing variations for compensating environment-induced clock drift in time synchronization , 2008, SIGMETRICS '08.

[8]  Robert Schmitt,et al.  Computed tomography for dimensional metrology , 2011 .

[9]  Ellis Meng,et al.  Micromachined Thermal Flow Sensors - A Review , 2012, Micromachines.

[10]  Simone Carmignato,et al.  Correction Strategies for the Use of a Conventional Micro-CT Cone Beam Machine for Metrology Applications , 2012 .

[11]  S. Klein,et al.  Design and uncertainty assessment of a setup for calibration of microfluidic devices down to 5 nL min−1 , 2013 .

[12]  N. Nguyen,et al.  Fundamentals and Applications of Microfluidics , 2002 .

[13]  Caglar Elbuken,et al.  Microfluidic system with integrated electroosmotic pumps, concentration gradient generator and fish cell line (RTgill-W1)--towards water toxicity testing. , 2009, Lab on a chip.

[14]  S. Marangoni,et al.  Structural and Biological Characterization of Two Crotamine Isoforms IV-2 and IV-3 Isolated from the Crotalus durissus cumanensis Venom , 2007, The protein journal.