Protein structural effects in gas phase ion/molecule reactions with diethylamine.

The relationship between gas-phase protein structure and ion/molecule reactivity is explored in comparisons between native and disulfide-reduced aprotinin, lysozyme, and albumin. Reactions are performed in the atmospheric-pressure inlet to a quadrupole mass spectrometer employing a novel capillary interface-reactor. In reactions with equal concentrations of diethylamine, multiply protonated molecules generated by electrospray ionization (ESI) of 'native' proteins shifted to lower charge states than did multiply protonated molecules from ESI of the disulfide-reduced counterparts, suggesting that the disulfide-reduced protein ions are less reactive than native protein ions of the same charge state. Differences in reactivity may arise from protonation of different amino acid residues and/or differences in the proximities of charge sites in the two molecules. These results suggest that the reactivity of multiply charged proteins can be significantly affected by their gas-phase structure.

[1]  Alan L. Rockwood,et al.  Thermally induced dissociation of ions from electrospray mass spectrometry , 1991 .

[2]  C. G. Edmonds,et al.  Tandem mass spectrometry of very large molecules: serum albumin sequence information from multiply charged ions formed by electrospray ionization. , 1991, Analytical chemistry.

[3]  W. Thormann,et al.  Purification of ovalbumin and lysozyme from a commercial product by recycling isotachophoresis. , 1991, Journal of chromatography.

[4]  B. Chait,et al.  Observation of the heme-globin complex in native myoglobin by electrospray-ionization mass spectrometry , 1991 .

[5]  K. Siu,et al.  Thermal denaturation of some proteins and its effect on their electrospray mass spectrat , 1991 .

[6]  G. Glish,et al.  Charge determination of product ions formed from collision-induced dissociation of multiply protonated molecules via ion/molecule reactions. , 1991, Analytical chemistry.

[7]  Richard D. Smith,et al.  Evidence of charge inversion in the reaction of singly charged anions with multiply charged macroions , 1991 .

[8]  Comparison of intensities between doubly charged ions [M + 2H]2+ and singly charged ions [M + H]+ of gramicidin S by electrospray mass spectrometry. , 1991, Rapid communications in mass spectrometry : RCM.

[9]  V. Katta,et al.  Conformational changes in proteins probed by hydrogen-exchange electrospray-ionization mass spectrometry. , 1991, Rapid communications in mass spectrometry : RCM.

[10]  C. G. Edmonds,et al.  Solvent-induced conformational changes of polypeptides probed by electrospray-ionization mass spectrometry. , 1991, Rapid communications in mass spectrometry : RCM.

[11]  Brian T. Chait,et al.  Probing conformational changes in proteins by mass spectrometry , 1990 .

[12]  C. G. Edmonds,et al.  New developments in biochemical mass spectrometry: electrospray ionization. , 1990, Analytical chemistry.

[13]  C. G. Edmonds,et al.  Primary sequence information from intact proteins by electrospray ionization tandem mass spectrometry. , 1990, Science.

[14]  C. G. Edmonds,et al.  Effect of reducing disulfide-containing proteins on electrospray ionization mass spectra. , 1990, Analytical chemistry.

[15]  D. E. Anderson,et al.  pH-induced denaturation of proteins: a single salt bridge contributes 3-5 kcal/mol to the free energy of folding of T4 lysozyme. , 1990, Biochemistry.

[16]  C. G. Edmonds,et al.  Collisional activation and dissociation of large multiply charged proteins produced by electrospray ionization , 1990 .

[17]  G. Glish,et al.  Reactions of Dimethylamine with Multiply Charged Ions of Cytochrome c , 1990 .

[18]  C. G. Edmonds,et al.  Collisional activation and collision-activated dissociation of large multiply charged polypeptides and proteins produced by electrospray ionization , 1990, Journal of the American Society for Mass Spectrometry.

[19]  Richard D. Smith,et al.  Peptide and protein analysis by electrospray ionization-mass spectrometry and capillary electrophoresis-mass spectrometry. , 1989, Analytical biochemistry.

[20]  C. G. Edmonds,et al.  Sequence determination of multiply charged peptide molecular ions by electrospray–ionization tandem mass spectrometry , 1989 .

[21]  Richard D. Smith,et al.  Collisional effects on the charge distribution of ions from large molecules, formed by electrospray‐ionization mass spectrometry , 1988 .

[22]  Richard D. Smith,et al.  Solvent effects on the charge distribution observed with electrospray ionization-mass spectrometry of large molecules , 1988 .

[23]  Charles J. Barinaga,et al.  Improved electrospray ionization interface for capillary zone electrophoresis-mass spectrometry , 1988 .

[24]  W. D. Phillips,et al.  Nuclear magnetic resonance study of the mechanism of reversible denaturation of lysozyme. , 1971, Journal of the American Chemical Society.

[25]  W. Cleland.  DITHIOTHREITOL, A NEW PROTECTIVE REAGENT FOR SH GROUPS. , 1964, Biochemistry.