Immuno-Flow Injection Analysis (FIA), Process Monitoring and Control

Flow injection analysis (FIA) is a technique that allows the automatic analysis of many samples and offers high sensitivity and accuracy. It has been combined with a variety of analytical methods such as chemical and biological assays. Immunoassays that recognize and bind antibody (Ab) to antigen (Ag) usually require multiple steps such as washing and incubation, which make them time-consuming. The FIA technology has been combined with the immunoassays to achieve high throughput and better control of the analytical process. A variety of immuno-FIAs have been developed and used for the monitoring of drugs, pesticides, steroids, hormones and other high-molecular weights products in biological processes. The use of immuno-FIA for process monitoring and control have often been reported in biotechnology. This article presents immuno-FIA technology based on homogeneous and heterogeneous immunoassays systematically and its application to the monitoring and control of biotechnological processes. Keywords: immunoassay; biotechnological process; flow injection analysis (FIA); immuno-FIA; process monitoring and control

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