A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells

[1]  N. Wu,et al.  Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation , 2021, bioRxiv.

[2]  Vineet D. Menachery,et al.  Furin Cleavage Site Is Key to SARS-CoV-2 Pathogenesis , 2020, bioRxiv.

[3]  Rebecca J. Loomis,et al.  Evaluation of the mRNA-1273 Vaccine against SARS-CoV-2 in Nonhuman Primates , 2020, The New England journal of medicine.

[4]  D. Matthews,et al.  Characterisation of the transcriptome and proteome of SARS-CoV-2 reveals a cell passage induced in-frame deletion of the furin-like cleavage site from the spike glycoprotein , 2020, Genome Medicine.

[5]  M. Hoffmann,et al.  A Multibasic Cleavage Site in the Spike Protein of SARS-CoV-2 Is Essential for Infection of Human Lung Cells , 2020, Molecular Cell.

[6]  Natacha S. Ogando,et al.  SARS-coronavirus-2 replication in Vero E6 cells: replication kinetics, rapid adaptation and cytopathology , 2020, bioRxiv.

[7]  Torsten Seemann,et al.  Isolation and rapid sharing of the 2019 novel coronavirus (SARS‐CoV‐2) from the first patient diagnosed with COVID‐19 in Australia , 2020, The Medical journal of Australia.

[8]  Vineet D. Menachery,et al.  Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States , 2020, Emerging infectious diseases.

[9]  T. Peto,et al.  Metagenomic Nanopore Sequencing of Influenza Virus Direct from Clinical Respiratory Samples , 2019, Journal of Clinical Microbiology.

[10]  G. Ebel,et al.  Mutations present in a low-passage Zika virus isolate result in attenuated pathogenesis in mice. , 2019, Virology.

[11]  G. Palacios,et al.  Growth-Adaptive Mutations in the Ebola Virus Makona Glycoprotein Alter Different Steps in the Virus Entry Pathway , 2018, Journal of Virology.

[12]  A. Wilm,et al.  LoFreq: a sequence-quality aware, ultra-sensitive variant caller for uncovering cell-population heterogeneity from high-throughput sequencing datasets , 2012, Nucleic acids research.