Study on separative structure of EnFET to detect acetylcholine

Abstract In this study, our laboratory used a separative structure of extended gate field effect transistor (EGFET) to detect pH value and constructed an enzyme modified field effect transistor (EnFET) by coupling them with a membrane containing immobilized acetylcholinesterase (AcChE). The enzyme-catalyzed hydrolysis reaction of acetylcholine seemed to be resulted in the pH change of the membrane due to the formation of acetic acid. This structure has a lot of advantages, such as being insensitive toward light, being simple to in its passivation and package, and the flexibility of shape in the extended gate area, etc. In addition, it is easier to fabricate and package the sensors, and the measurement is simple for the application of disposable biosensor.

[1]  J. Janata,et al.  Field effect transistor sensitive to penicillin , 1980 .

[2]  T. Scheper,et al.  Computational neural networks for the evaluation of biosensor FIA measurements , 1997 .

[3]  Wen-Yaw Chung,et al.  Study on extended gate field effect transistor with tin oxide sensing membrane , 2000 .

[4]  Andrey Bratov,et al.  Enzyme semiconductor sensor based on butyrylcholinesterase , 1991 .

[5]  W. Torbicz,et al.  PH-ISFET BASED UREA BIOSENSOR , 1997 .

[6]  Thomas Kullick,et al.  Application of enzyme-field effect transistor sensor arrays as detectors in a flow-injection analysis system for simultaneous monitoring of medium components. Part II. Monitoring of cultivation processes , 1995 .

[7]  S. Gondo,et al.  Immobilization of enzyme to platinum electrode and its use as enzyme electrode , 1991 .

[8]  H. Müller,et al.  Photolithographically patterned enzyme membranes for the detection of pesticides and copper(II) based on enzyme inhibition , 1993 .

[9]  Bernd Hitzmann,et al.  Application of enzyme field-effect transistor sensor arrays as detectors in a flow-injection system for simultaneous monitoring of medium components. Part I. Preparation and calibration. , 1994 .

[10]  Nicole Jaffrezic-Renault,et al.  Sensitive detection of pesticides using a differential ISFET-based system with immobilized cholinesterases , 1993 .

[11]  Maria Pia Sammartino,et al.  Organophosphorus pesticide (Paraoxon) analysis using solid state sensors , 1996 .

[12]  A. Poghossian,et al.  Method of fabrication of ISFET-based biosensors on an Si–SiO2–Si structure , 1997 .

[13]  Chang-Soo Kim,et al.  ISFET glucose sensor based on a new principle using the electrolysis of hydrogen peroxide , 1997 .

[14]  L. Park,et al.  The Improvement of Sensing Performance of ISFET Glucose and Sucrose Sensors by Using Platinum Electrode and Photo-crosslinkable Polymers , 1997 .

[15]  H. Müller,et al.  Advances in photolithographically fabricated ENFET membranes , 1994 .