parvovirus B19 structural proteins. baculovirus recombinants expressing human Assembly of empty capsids by using

andAcB19VP2L(as described in reference 3). In thesame manner, the VP1 gene was cloned in the plasmid vectorpAcAS3andcotransfected withAcB19VP2LDNA.Uponrecombi-nation in insect cells, the double-recombinant baculovirusAcB19VP12L was isolated. ORF, open reading frame; Ac, A.californica nuclear polyhedrosis virus sequences flanking the plOgene; plOp, plO promoter; php, polyhedrin promoter; hsp, D.melanogaster

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