Occurrence of Pasteurellaceae and Neisseriaceae bacteria in the pharyngeal and respiratory tract of dogs and cats - Short communication.

The occurrence of members of the Pasteurellaceae and Neisseriaceae families was studied in dogs and cats. A total of 110 nasal and pharyngeal swab samples from 47 dogs and 8 cats were collected. Most of the strains were identified by 16S rDNA sequencing, except Frederiksenia canicola and Pasteurella multocida where species-specific polymerase chain reactions were applied. The most frequently isolated species was F. canicola, which occurred only in dogs, mainly in the pharyngeal cavity. The second commonest bacterium, P. multocida was found in both types of samples and in both hosts. Other species from the family Pasteurellaceae, such as Haemophilus haemoglobinophilus, Pasteurella canis and P. dagmatis, were detected only in dogs. All isolated species belonging to the family Neisseriaceae, mainly representing Neisseria weaveri, were found only in the pharyngeal cavity. Neisseria weaveri and N. zoodegmatis could be detected in both hosts. Neisseria dumasiana and N. canis were isolated from dogs, while N. shayeganii only from a cat. For phylogenetic analysis, rpoB gene sequencing was performed, where the strains were on monophyletic branches and clearly separated from each other. In this study, recently described species such as F. canicola, N. shayeganii and N. dumasiana were detected that had never been isolated in Hungary before.

[1]  T. Shinha Cellulitis and Bacteremia due to Neisseria weaveri following a dog bite , 2018, IDCases.

[2]  C. Hurst Opportunistic Bacteria Associated with Mammalian Livestock Disease , 2018 .

[3]  W. Wolfgang,et al.  Neisseria dumasiana sp. nov. from human sputum and a dog's mouth. , 2017, International journal of systematic and evolutionary microbiology.

[4]  M. Bertelsen,et al.  OCCURRENCE OF PASTEURELLACEAE BACTERIA IN THE ORAL CAVITY OF SELECTED MARSUPIAL SPECIES , 2012, Journal of Zoo and Wildlife Medicine.

[5]  Sudhir Kumar,et al.  MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. , 2016, Molecular biology and evolution.

[6]  M. Bertelsen,et al.  OCCURRENCE OF PASTEURELLACEAE BACTERIA IN THE ORAL CAVITY OF THE TASMANIAN DEVIL (SARCOPHILUS HARRISII). , 2015, Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians.

[7]  J. Weese,et al.  Characterization of the oral microbiota of healthy cats using next-generation sequencing. , 2014, Veterinary journal.

[8]  B. Korczak,et al.  Frederiksenia canicola gen. nov., sp. nov. isolated from dogs and human dog-bite wounds , 2014, Antonie van Leeuwenhoek.

[9]  B. Korczak,et al.  Identification of animal Pasteurellaceae by MALDI-TOF mass spectrometry. , 2012, Journal of microbiological methods.

[10]  H. Kippenes,et al.  First Reported Isolation of Neisseria canis from a Deep Facial Wound Infection in a Dog , 2011, Journal of Clinical Microbiology.

[11]  S. Gronow,et al.  Neisseria wadsworthii sp. nov. and Neisseria shayeganii sp. nov., isolated from clinical specimens. , 2011, International journal of systematic and evolutionary microbiology.

[12]  L. Makrai,et al.  Characterisation of Pasteurella dagmatis-like isolates recovered from the feline oral cavity. , 2010, Veterinary microbiology.

[13]  B. Korczak,et al.  Phylogeny of the family Pasteurellaceae based on rpoB sequences. , 2004, International journal of systematic and evolutionary microbiology.

[14]  C. Hart,et al.  A case of lower respiratory tract infection caused by Neisseria weaveri and review of the literature. , 2002, The Journal of infection.

[15]  P. Ahrens,et al.  Development of a PCR test for identification of Haemophilus somnus in pure and mixed cultures. , 1998, Veterinary microbiology.

[16]  J. Papadimitriou,et al.  Development of PCR Assays for Species- and Type-Specific Identification of Pasteurella multocidaIsolates , 1998, Journal of Clinical Microbiology.

[17]  P. Ossent,et al.  Isolation and characterization of group EF-4 bacteria from various lesions in cat, dog and badger. , 1993, Zentralblatt fur Bakteriologie : international journal of medical microbiology.

[18]  S. Neill,et al.  Pathological changes associated with group EF-4 bacteria in the lungs of a dog and a cat , 1982, Veterinary Record.

[19]  J. G. Allen,et al.  The isolation of group EF-4 bacteria from a case of granulomatous pneumonia in a tiger cub. , 1980, Australian veterinary journal.