Detection of genetically diverse human immunodeficiency virus type 1 group M and O isolates by PCR

A panel of 136 genetically diverse group M and 5 group O adult isolates from outside the United States and Europe were evaluated by PCR with the Roche AMPLICOR HIV-1 test, a modified version of the AMPLICOR HIV-1 test, and a new primer pair/probe system. Detection of some of these isolates was less efficient with the AMPLICOR HIV-1 test; however, the assay was significantly improved by reducing the sample input and lowering the annealing temperature. The new primer pair/probe set detected 140 of 141 isolates, including the 5 group O isolates that were not detected with either of the AMPLICOR HIV-1 test formats.

[1]  John W. Mellors,et al.  Human retroviruses and AIDS 1996. A compilation and analysis of nucleic acid and amino acid sequences , 1997 .

[2]  P. Sullivan,et al.  HIV-1 group O virus identified for the first time in the United States. , 1996, Emerging infectious diseases.

[3]  D J Hu,et al.  The emerging genetic diversity of HIV. The importance of global surveillance for diagnostics, research, and prevention. , 1996, JAMA.

[4]  D. Ho,et al.  Genetic analysis of human immunodeficiency virus type 1 strains from patients in Cyprus: identification of a new subtype designated subtype I , 1995, Journal of virology.

[5]  F. Brun-Vézinet,et al.  Genetic diversity and HIV detection by polymerase chain reaction , 1995, The Lancet.

[6]  B. Korber,et al.  Variability of human immunodeficiency virus type 1 group O strains isolated from Cameroonian patients living in France , 1995, Journal of virology.

[7]  C. Loveday,et al.  HIV type 1 sequence subtype G transmission from mother to infant: failure of variant sequence species to amplify in the Roche Amplicor Test. , 1995, AIDS research and human retroviruses.

[8]  T. Leitner,et al.  Yet another subtype of HIV type 1? , 1995, AIDS research and human retroviruses.

[9]  C. Luo,et al.  HIV-1 subtype C in China , 1995, The Lancet.

[10]  H. Jaffe,et al.  Sensitivity of United States HIV antibody tests for detection of HIV-1 group O infections , 1994, The Lancet.

[11]  F. Brun-Vézinet,et al.  HIV-1/HIV-2 seronegativity in HIV-1 subtype 0 infected patients , 1994, The Lancet.

[12]  S. Knapp,et al.  A new subtype of human immunodeficiency virus type 1 (MVP-5180) from Cameroon , 1994, Journal of virology.

[13]  D. Burke,et al.  Phylogenetic analysis of gag genes from 70 international HIV‐1 isolates provides evidence for multiple genotypes , 1993, AIDS.

[14]  H. Gayle,et al.  Highly specific V3 peptide enzyme immunoassay for seretyping HIV‐1 specimens from Thailand , 1993, AIDS.

[15]  J. Spadoro,et al.  Using PCR for detection of HIV-1 infection , 1992 .

[16]  L. Heyndrickx,et al.  Isolation and partial characterization of an unusual human immunodeficiency retrovirus from two persons of west-central African origin , 1990, Journal of virology.

[17]  S. Dewhurst,et al.  Differences in cytopathogenicity and host cell range among infectious molecular clones of human immunodeficiency virus type 1 simultaneously isolated from an individual , 1988, Journal of virology.

[18]  C Y Ou,et al.  DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells. , 1988, Science.

[19]  R. Redfield,et al.  Genetic variation in HTLV-III/LAV over time in patients with AIDS or at risk for AIDS. , 1986, Science.

[20]  R. Gallo,et al.  Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS. , 1984, Science.

[21]  J. Chermann,et al.  Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS). , 1983, Science.

[22]  T. A. Hagan,et al.  Detection , Isolation , and Continuous Production of Cytopathic Retroviruses ( HTLV-III ) from Patients with AIDS and Pre-AIDS , 2022 .