Eupergit® C, a carrier for immobilization of enzymes of industrial potential

Eupergit® C is a carrier consisting of macroporous beads for immobilizing enzymes of industrial potential for the production of fine chemicals and pharmaceuticals. Various enzymes immobilized on Eupergit® C are reviewed in comparison with other carrier materials in terms of the operational stability of the respective biocatalysts at substrate concentrations realistic for industrial production. Other aspects of relevance in that field, such as the demand for purity of enzyme to be immobilized or type of reactor optimal for a given application, are also discussed. An automatic reactor simulating, at laboratory scale, the performance of an industrial stirred tank reactor (STR) is described, and its utilization for evaluating the performance of immobilized enzymes is shown.

[1]  E. Keinan,et al.  Thermostable enzymes in organic synthesis. 2. Asymmetric reduction of ketones with alcohol dehydrogenase from Thermoanaerobium brockii , 1986 .

[2]  C. Hill,et al.  Hydrolysis of menhaden oil by a Candida cylindracea lipase immobilized in a hollow-fiber reactor. , 1999, Biotechnology and bioengineering.

[3]  S. Fager,et al.  Glyoxylic acid production using immobilized glycolate oxidase and catalase. , 1994, Bioorganic & medicinal chemistry.

[4]  J. Kálal,et al.  Methacrylate gels with epoxide groups as supports for immobilization of enzymes in pH range 3-12. , 1978, Biochimica et biophysica acta.

[5]  P. Monsan,et al.  Enzymatic synthesis of α‐butylglucoside lactate: A new α‐hydroxy acid derivative , 1999 .

[6]  M. Kula,et al.  Improved purification of an (R)‐oxynitrilase from Linum usitatissimum (flax) and investigation of the substrate range , 1993, Biotechnology and applied biochemistry.

[7]  E. Katchalski,et al.  A Water-insoluble Trypsin Derivative and its Use as a Trypsin Column , 1960, Nature.

[8]  M. Dawson,et al.  An efficient process for production of N-acetylneuraminic acid using N-acetylneuraminic acid aldolase. , 1997, Enzyme and microbial technology.

[9]  E. Vulfson,et al.  Application of enzymes to the synthesis of surfactants. , 1995, Trends in biotechnology.

[10]  J Hinrichs,et al.  pH gradients in immobilized amidases and their influence on rates and yields of beta-lactam hydrolysis. , 1999, Biotechnology and bioengineering.

[11]  Takao Mori,et al.  Studies on continuous enzyme reactions. IV. Preparation of a DEAE‐sephadex–aminoacylase column and continuous optical resolution of acyl‐DL‐amino acids , 1967 .

[12]  H. Yamada,et al.  Microbial transformations of nitriles , 1989 .

[13]  I. Silman,et al.  Water-insoluble derivatives of enzymes, antigens, and antibodies. , 1966, Annual review of biochemistry.

[14]  R. Keller,et al.  [47] Production of 5′-ribonucleotides using immobilized 5′-phosphodiesterase , 1987 .

[15]  Susanne Rugh,et al.  [33] Industrial-scale production and application of immobilized glucose isomerase , 1987 .

[16]  Alexander M. Klibanov,et al.  Enzymatic reactions in organic media , 1996 .

[17]  M. Schneider,et al.  Methods for the immobilization of lipases and their use for ester synthesis , 1997 .

[18]  S. Riva.  Regioselectivity of hydrolases in organic media , 1996 .

[19]  S. Colombié,et al.  Water activity control: a way to improve the efficiency of continuous lipase esterification. , 1998, Biotechnology and Bioengineering.

[20]  I. Purvis,et al.  Enzymatic production of optically pure (2'R-cis)-2'-deoxy-3'-thiacytidine (3TC, lamivudine): a potent anti-HIV agent. , 1993, Enzyme and microbial technology.

[21]  M R Kula,et al.  Continuous enzymatic transformation in an enzyme-membrane reactor with simultaneous NADH regeneration. , 1987, Methods in enzymology.

[22]  G. Carrea,et al.  Optimization of Pseudomonas cepacia lipase preparations for catalysis in organic solvents. , 1999, Biotechnology and bioengineering.

[23]  Gao,et al.  Regioselective synthesis of ethoxylated glycoside esters using beta-glucosidase in supersaturated solutions and lipases in organic solvents , 1998, Biotechnology and bioengineering.

[24]  E. Katchalski‐Katzir,et al.  Immobilized enzymes--learning from past successes and failures. , 1993, Trends in biotechnology.

[25]  V. Kasche,et al.  Enzyme catalyzed biotransformations in aqueous two‐phase systems with precipitated substrate and/or product , 1995, Biotechnology and bioengineering.