Establishment and Characterization of an Acute Lymphocytic Leukemia Cell Line Expressing CD13 and CD33 with a Complex Philadelphia Translocation

Objective To investigate the pathogenesis of Philadelphia (Ph)-positive acute lymphocytic leukemia (ALL), we established a lymphoblastoid cell line. Methods Bone marrow cells from a patient with Ph-positive ALL were enriched by Ficoll-Hypaque centrifugation and cultured in medium with fetal calf serum. Materials The mononuclear cells of bone marrow aspirate were obtained from an adult man with ALL after he experienced relapse following induction therapy including imatinib mesylate. Results The cell line termed TNA-M was established, carrying a three-way Ph translocation involving two chromosome 9s and one chromosome 22 as a sole karyotypic abnormality. Furthermore, the cells were positive for CD13 and CD33 in addition to CD19, CD22 and CD79a antigens. Conclusion This unique cell line is expected to be a valuable tool for understanding the pathogenesis of Ph-positive ALL.

[1]  S. Okabe,et al.  Establishment of a new Philadelphia chromosome-positive acute lymphoblastic leukemia cell line (SK-9) with T315I mutation. , 2010, Experimental hematology.

[2]  J. Cayuela,et al.  Imatinib combined with induction or consolidation chemotherapy in patients with de novo Philadelphia chromosome – positive acute lymphoblastic leukemia : results of the GRAAPH-2003 study , 2006 .

[3]  P. Campbell,et al.  Acquired mutation of the tyrosine kinase JAK2 in human myeloproliferative disorders , 2005, The Lancet.

[4]  R. Giavazzi,et al.  Gemtuzumab ozogamicin (Mylotarg) has therapeutic activity against CD33+ acute lymphoblastic leukaemias in vitro and in vivo , 2005, British journal of haematology.

[5]  Z. Estrov,et al.  Outcome of Philadelphia Chromosome-Positive Adult Acute Lymphoblastic Leukemia , 2000, Leukemia & lymphoma.

[6]  H. Drexler,et al.  Leukemia cell lines: in vitro models for the study of Philadelphia chromosome-positive leukemia. , 1999, Leukemia research.

[7]  N. Nara,et al.  Establishment of a double Philadelphia chromosome-positive acute lymphoblastic leukemia-derived cell line, TMD5: effects of cytokines and differentiation inducers on growth of the cells. , 1999, Leukemia research.

[8]  H. Dadi,et al.  Cloning and characterization of the human homolog of mouse Jak2. , 1998, Blood.

[9]  Z. Estrov,et al.  Role of granulocyte‐macrophage colony‐stimulating factor in Philadelphia (Ph1)‐positive acute lymphoblastic leukemia: Studies on two newly established Ph1‐positive acute lymphoblastic leukemia cell lines (Z‐119 and Z‐181) , 1996, Journal of cellular physiology.

[10]  M. Andreeff,et al.  Myeloid markers in adult acute lymphocytic leukemia. Correlations with patient and disease characteristics and with prognosis , 1995, Cancer.

[11]  C. Bloomfield,et al.  Clinical significance of the BCR-ABL fusion gene in adult acute lymphoblastic leukemia: a Cancer and Leukemia Group B Study (8762). , 1992, Blood.