Insufficient and excessive N fertilizer input reduces maize root mass across soil types

Abstract Quantifying maize root response to nitrogen (N) fertilizer, soil texture, and weather is crucial to understand complex soil-root-plant processes. We performed a 2-year x 4 locations (sand content range: 5–95%) x N treatments (range: 0 to 336 kg N ha−1) field experiment in Iowa, U.S. to (1) determine the response of root traits to N fertilizer, and (2) develop generalized functions to aid understanding and prediction of root mass and root to shoot (R:S) ratio. Deep root samples (0−210 cm, increments of 30 cm) were collected using the soil core approach at early to middle grain fill period and quantified root mass, length, and N and C concentrations. In addition, yield and shoot biomass was measured. Root traits and yield had different responses to N fertilizer input. Root mass was maximized at 168 kg N ha−1; zero and excessive N fertilization decreased root mass by 33 and 17 %, respectively. Nitrogen fertilizer significantly affected root traits only in the top 30 cm soil layer. Soil texture affected root traits in a dry year (root mass was positively associated with silt and clay), but not in a wet year, suggesting that soil moisture overwhelms the effect of texture. The combined data (N rates x locations x years) revealed a negative relationship between R:S ratio and yield. This resulted in a new set of equations (e.g., upper bound R:S = e(–1.5 – 0.04*yield)) that can replace the constant R:S approach used in the literature. Yield, which is commonly measured, integrates the effects of environment, management, and genetic variation; hence the proposed equations can be widely applied. This study provides evidence that different plant traits are maximized at different levels of mineral N nutrition. Results can enhance biophysical models and prediction of R:S ratio.

[1]  C. Jones,et al.  Effect of Soil Texture on Critical Bulk Densities for Root Growth , 1983 .

[2]  Aiwang Duan,et al.  Distribution of roots and root length density in a maize/soybean strip intercropping system , 2010 .

[3]  A. Blackmer,et al.  Comparison of Models for Describing; Corn Yield Response to Nitrogen Fertilizer , 1990 .

[4]  Neil I. Huth,et al.  Enhancing APSIM to simulate excessive moisture effects on root growth , 2019, Field Crops Research.

[5]  G. Mi,et al.  Effects of Nitrogen Application on Root Length and Grain Yield of Rain-Fed Maize under Different Soil Types , 2016 .

[6]  T. Amado,et al.  Phosphorus Fertilizer Placement and Tillage Affect Soybean Root Growth and Drought Tolerance , 2017 .

[7]  Marie-France Dignac,et al.  Is soil carbon mostly root carbon? Mechanisms for a specific stabilisation , 2005, Plant and Soil.

[8]  C. C. Deuner,et al.  Influence of continuous cropping on corn and soybean pathogens. , 2017 .

[9]  Genetic variation in the critical specific leaf nitrogen maximising yield among modern maize hybrids , 2015 .

[10]  Chris Smith,et al.  Modelling the growth and water uptake function of plant root systems: a review , 2004 .

[11]  David B. Lobell,et al.  How much will precision nitrogen management pay off? An evaluation based on simulating thousands of corn fields over the US Corn-Belt , 2019, Field Crops Research.

[12]  R. Belford,et al.  Growth, development and light interception of old and modern wheat cultivars in a Mediterranean-type environment , 1989 .

[13]  J. Lynch,et al.  Low Crown Root Number Enhances Nitrogen Acquisition from Low-Nitrogen Soils in Maize1[W][OPEN] , 2014, Plant Physiology.

[14]  P. Maenhout,et al.  Maize root biomass and architecture depend on site but not on variety: Consequences for prediction of C inputs and spread in topsoil based on root-to-shoot ratios , 2020 .

[15]  Javed Iqbal,et al.  Extreme weather‐year sequences have nonadditive effects on environmental nitrogen losses , 2018, Global change biology.

[16]  G. Lemaire,et al.  Relationships between dynamics of nitrogen uptake and dry matter accumulation in maize crops. Determination of critical N concentration , 1999, Plant and Soil.

[17]  R Core Team,et al.  R: A language and environment for statistical computing. , 2014 .

[18]  Jianbo Shen,et al.  Combined Applications of Nitrogen and Phosphorus Fertilizers with Manure Increase Maize Yield and Nutrient Uptake via Stimulating Root Growth in a Long-Term Experiment , 2016 .

[19]  J. Six,et al.  Tracing Root vs. Residue Carbon into Soils from Conventional and Alternative Cropping Systems , 2010 .

[20]  Raziel A. Ordóñez,et al.  A solution for sampling position errors in maize and soybean root mass and length estimates. , 2018 .

[21]  W. Cheng.  Rhizosphere priming effect: Its functional relationships with microbial turnover, evapotranspiration, and C–N budgets , 2009 .

[22]  Akira Yamauchi,et al.  Root Development and Nutrient Uptake , 2006 .

[23]  J. Six,et al.  Whole-profile soil organic matter content, composition, and stability under cropping systems that differ in belowground inputs , 2020 .

[24]  Paul C. Struik,et al.  Temporal dynamics of light and nitrogen vertical distributions in canopies of sunflower, kenaf and cynara , 2011 .

[25]  Daniel T. Walters,et al.  Maize Root Biomass and Net Rhizodeposited Carbon , 2006 .

[26]  H. Janzen,et al.  An approach for estimating net primary productivity and annual carbon inputs to soil for common agricultural crops in Canada , 2007 .

[27]  Raziel A. Ordóñez,et al.  Yield response to heat stress as affected by nitrogen availability in maize , 2015 .

[28]  I. Wesström,et al.  Response of maize root growth to irrigation and nitrogen management strategies in semi-arid loamy sandy soil , 2017 .

[29]  A. Srivastava,et al.  Quantitative Separation of Roots from Compacted Soil Profiles by the Hydropneumatic Elutriation System1 , 1982 .

[30]  P. White,et al.  Phenotypic plasticity of the maize root system in response to heterogeneous nitrogen availability , 2014, Planta.

[31]  Brian McConkey,et al.  Root distribution by depth for temperate agricultural crops , 2016 .

[32]  H. Mooney,et al.  Resource Limitation in Plants-An Economic Analogy , 1985 .

[33]  M. Hawkesford,et al.  Exploiting genetic variation in nitrogen use efficiency for cereal crop improvement , 2019, Current opinion in plant biology.

[34]  M. A. Bolinder,et al.  Estimating shoot to root ratios and annual carbon inputs in soils for cereal crops , 1997 .

[35]  S. Yip,et al.  Association of High Myopia with Crystallin Beta A4 (CRYBA4) Gene Polymorphisms in the Linkage-Identified MYP6 Locus , 2012, PloS one.

[36]  F. Below,et al.  Changes in Nitrogen Use Traits Associated with Genetic Improvement for Grain Yield of Maize Hybrids Released in Different Decades , 2013 .

[37]  Walter J. Horst,et al.  Growth and distribution of maize roots under nitrogen fertilization in plinthite soil , 1999 .

[38]  D. W. Stewart,et al.  Root mass distribution under conventional and conservation tillage , 1996 .

[39]  R. B. Jackson,et al.  THE VERTICAL DISTRIBUTION OF SOIL ORGANIC CARBON AND ITS RELATION TO CLIMATE AND VEGETATION , 2000 .

[40]  J. Hopmans,et al.  Transient three-dimensional modeling of soil water and solute transport with simultaneous root growth, root water and nutrient uptake , 1998, Plant and Soil.

[41]  D. Laird,et al.  Nitrogen fertilizer effects on soil carbon balances in midwestern U.S. agricultural systems. , 2009, Ecological applications : a publication of the Ecological Society of America.

[42]  C. Swanton,et al.  Tillage alters corn root distribution in coarse-textured soil , 1998 .

[43]  A. Warrick.  Soil Water Dynamics , 2003 .

[44]  C. Pittelkow,et al.  Linking Nitrogen Losses With Crop Productivity in Maize Agroecosystems , 2018, Front. Sustain. Food Syst..

[45]  B. Basso,et al.  Can multi-strategy management stabilize nitrate leaching under increasing rainfall? , 2019, Environmental Research Letters.

[46]  E. L. Anderson.  Tillage and N fertilization effects on maize root growth and root:shoot ratio , 1988, Plant and Soil.

[47]  M. Castellano,et al.  Can mineralization of soil organic nitrogen meet maize nitrogen demand? , 2017, Plant and Soil.

[48]  J. Lynch,et al.  Maize root growth angles become steeper under low N conditions , 2013 .

[49]  C. Pittelkow,et al.  Simulating nitrogen management impacts on maize production in the U.S. Midwest , 2018, PloS one.

[50]  Fusuo Zhang,et al.  Ideotype root architecture for efficient nitrogen acquisition by maize in intensive cropping systems , 2010, Science China Life Sciences.

[51]  M. Werger,et al.  Patterns of light and nitrogen distribution in relation to whole canopy carbon gain in C3 and C4 mono- and dicotyledonous species , 1995, Oecologia.

[52]  N. I. Huth,et al.  SWIM3: Model Use, Calibration, and Validation , 2012 .

[53]  James W. Jones,et al.  Towards a multiscale crop modelling framework for climate change adaptation assessment , 2020, Nature Plants.

[54]  S. A. Barber,et al.  Soil Nutrient Bioavailability: A Mechanistic Approach , 1984 .

[55]  J. Lynch,et al.  Evolution of US maize (Zea mays L.) root architectural and anatomical phenes over the past 100 years corresponds to increased tolerance of nitrogen stress , 2015, Journal of experimental botany.

[56]  S. Abiven,et al.  Maize and wheat root biomass, vertical distribution, and size class as affected by fertilization intensity in two long-term field trials , 2018 .

[57]  A. Dobermann,et al.  Agroecosystems, Nitrogen-use Efficiency, and Nitrogen Management , 2002, Ambio.

[58]  C. Messina,et al.  Simultaneous gains in grain yield and nitrogen efficiency over 70 years of maize genetic improvement , 2019, Scientific Reports.

[59]  Raziel A. Ordóñez,et al.  Predicting crop yields and soil‐plant nitrogen dynamics in the US Corn Belt , 2020 .

[60]  P. White,et al.  A Large and Deep Root System Underlies High Nitrogen-Use Efficiency in Maize Production , 2015, PloS one.

[61]  N. Leon,et al.  Breeding Maize for a Bioeconomy: A Literature Survey Examining Harvest Index and Stover Yield and Their Relationship to Grain Yield , 2010 .

[62]  Synchrony of net nitrogen mineralization and maize nitrogen uptake following applications of composted and fresh swine manure in the Midwest U.S. , 2012, Nutrient Cycling in Agroecosystems.

[63]  Bahman Eghball,et al.  Root Development and Nitrogen Influx of Corn Genotypes Grown under Combined Drought and Nitrogen Stresses , 1993 .

[64]  Liu Zhiming,et al.  Evolution of roots to improve water and nitrogen use efficiency in maize elite inbred lines released during different decades in China , 2019, Agricultural Water Management.

[65]  N. Fageria,et al.  The Role of Mineral Nutrition on Root Growth of Crop Plants , 2011 .

[66]  C. Messina,et al.  N distribution in maize plant as a marker for grain yield and limits on its remobilization after flowering , 2013 .

[67]  Laura F. Gentry,et al.  Identifying factors controlling the continuous corn yield penalty , 2013 .

[68]  M. Walters,et al.  Optimal partitioning theory revisited: nonstructural carbohydrates dominate root mass responses to nitrogen. , 2010, Ecology.

[69]  Matthew J. Helmers,et al.  Modeling the impacts of climate change on nitrogen losses and crop yield in a subsurface drained field , 2015, Climatic Change.

[70]  Roger W. Elmore,et al.  Can crop simulation models be used to predict local to regional maize yields and total production in the U.S. Corn Belt , 2016 .

[71]  D. Eissenstat,et al.  Costs and benefits of constructing roots of small diameter , 1992 .

[72]  W. Rawls,et al.  Soil Water Characteristic Estimates by Texture and Organic Matter for Hydrologic Solutions , 2006 .

[73]  Xianju Lu,et al.  Modeling Soil Type Effects to Improve Rainfed Corn Yields in Northeast China , 2016 .

[74]  John E. Sawyer,et al.  Modeling Long-Term Corn Yield Response to Nitrogen Rate and Crop Rotation , 2016, Front. Plant Sci..

[75]  Armen R. Kemanian,et al.  Can Crop Models Identify Critical Gaps in Genetics, Environment, and Management Interactions? , 2020, Frontiers in Plant Science.

[76]  M. Castellano,et al.  A Systems Modeling Approach to Forecast Corn Economic Optimum Nitrogen Rate , 2018, Front. Plant Sci..

[77]  G. Robertson,et al.  The contribution of maize cropping in the Midwest USA to global warming: A regional estimate , 2011 .

[78]  Jan Vos,et al.  A flexible sigmoid function of determinate growth. , 2003, Annals of botany.

[79]  María E. Otegui,et al.  Kernel number determination differs among maize hybrids in response to nitrogen , 2008 .

[80]  W. Jackson,et al.  Root Distribution of Corn: The Effect of Nitrogen Fertilization , 1994 .

[81]  S. Archontoulis,et al.  A deeper look at the relationship between root carbon pools and the vertical distribution of the soil carbon pool , 2017 .

[82]  V. Sadras,et al.  Effect of Nitrogen Content on the Photosynthetic Characteristics of Sunflower Leaves , 1993 .

[83]  Raziel A. Ordóñez,et al.  Root to shoot and carbon to nitrogen ratios of maize and soybean crops in the US Midwest , 2020 .

[84]  Fusuo Zhang,et al.  Shoot growth potential drives N uptake in maize plants and correlates with root growth in the soil , 2010 .

[85]  J. Six,et al.  Maximum soil organic carbon storage in Midwest U.S. cropping systems when crops are optimally nitrogen-fertilized , 2017, PloS one.

[86]  G. Hoogenboom.  MODELING ROOT GROWTH AND IMPACT ON PLANT DEVELOPMENT , 1999 .

[87]  Raziel A. Ordóñez,et al.  Maize and soybean root front velocity and maximum depth in Iowa, USA , 2018 .

[88]  Raziel A. Ordóñez,et al.  Maize root distributions strongly associated with water tables in Iowa, USA , 2019, Plant and Soil.

[89]  M. Goss,et al.  Root growth and distribution in relation to nutrient availability and uptake , 1993 .

[90]  Chunjian Li,et al.  Temporal and Spatial Profiling of Root Growth Revealed Novel Response of Maize Roots under Various Nitrogen Supplies in the Field , 2012, PloS one.

[91]  Navreet K. Mahal,et al.  Nitrogen Fertilizer Suppresses Mineralization of Soil Organic Matter in Maize Agroecosystems , 2019, Front. Ecol. Evol..

[92]  Bijay S Singh.  Are Nitrogen Fertilizers Deleterious to Soil Health , 2018 .

[93]  Dan Zaslavsky,et al.  Soil water dynamics , 1960 .

[94]  Jonathan P Lynch,et al.  Steep, cheap and deep: an ideotype to optimize water and N acquisition by maize root systems. , 2013, Annals of botany.

[95]  F. G. González,et al.  Water and radiation use efficiencies in maize: Breeding effects on single-cross Argentine hybrids released between 1980 and 2012 , 2020 .

[96]  Xu Zhao,et al.  Nitrogen rate strategies for reducing yield-scaled nitrous oxide emissions in maize , 2017 .