Osteoinduction by Functionally Graded Apatites of Bovine Origin Loaded with Bone Morphogenetic Protein-2

Bioresorbable and functionally graded apatites (fg-HAp) ceramics, which are characterized by gradations in crystallinity and the grain size of hydroxyapatite (HAp:Ca10(PO4)6(OH)2), were designed using bovine bone by the calcination and partial dissolution–precipitation method. The fg-HAp ceramics had macropores of 100–600 μm originated from spongy bone, and micropores of 10–160 nm. Fg-HAp ceramics loaded with recombinant human bone morphogenetic protein-2 (rhBMP-2/fg-HAp) were implanted into the subcutaneous tissue of rats. Eight weeks after the implantation, the surface and bulk degradations of the fg-HAp-containing body fluid occured, and small pieces of fg-HAp were incorporated into the induced bone and fatty marrow, suggesting that osteoinduction occurred in conjunction with bone remodeling. The rhBMP-2/fg-HAp ceramics developed could become a resorbable biomimetic material with fast bioresorption and osteoinduction characteristics.

[1]  Haim Tal,et al.  Biomaterial resorption rate and healing site morphology of inorganic bovine bone and beta-tricalcium phosphate in the canine: a 24-month longitudinal histologic study and morphometric analysis. , 2004, The International journal of oral & maxillofacial implants.

[2]  A. Reddi,et al.  The application of bone morphogenetic proteins to dental tissue engineering , 2003, Nature Biotechnology.

[3]  F. Bloemers,et al.  Autologous bone versus calcium-phosphate ceramics in treatment of experimental bone defects. , 2003, Journal of biomedical materials research. Part B, Applied biomaterials.

[4]  R. Haas,et al.  Sinus grafting with autogenous platelet-rich plasma and bovine hydroxyapatite. A histomorphometric study in minipigs. , 2003, Clinical oral implants research.

[5]  L. Sennerby,et al.  A prospective 1-year clinical and radiographic study of implants placed after maxillary sinus floor augmentation with bovine hydroxyapatite and autogenous bone. , 2002, Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons.

[6]  S. Nakamura,et al.  Enhanced osteobonding by negative surface charges of electrically polarized hydroxyapatite. , 2001, Journal of biomedical materials research.

[7]  I. Asahina,et al.  Evaluation of ceramics composed of different hydroxyapatite to tricalcium phosphate ratios as carriers for rhBMP-2. , 2001, Biomaterials.

[8]  Jui-Sheng Sun,et al.  Preparation of ?TCP/HAP biphasic ceramics with natural bone structure by heating bovine cancellous bone with the addition of (NH4)2HPO4 , 2000 .

[9]  B. Nies,et al.  Chemical and physicochemical characterization of porous hydroxyapatite ceramics made of natural bone. , 2000, Biomaterials.

[10]  R. Bareille,et al.  Various evaluation techniques of newly formed bone in porous hydroxyapatite loaded with human bone marrow cells implanted in an extra-osseous site. , 2000, Biomaterials.

[11]  W. Bonfield,et al.  Quantification of bone ingrowth within bone-derived porous hydroxyapatite implants of varying density , 1999, Journal of materials science. Materials in medicine.

[12]  A. Minamide,et al.  Experimental spinal fusion using sintered bovine bone coated with type I collagen and recombinant human bone morphogenetic protein-2. , 1999, Spine.

[13]  T. Yoshimoto,et al.  Use of porous hydroxyapatite graft containing recombinant human bone morphogenetic protein-2 for cervical fusion in a caprine model. , 1999, Journal of neurosurgery.

[14]  M. Murata,et al.  Carrier-dependency of cellular differentiation induced by bone morphogenetic protein in ectopic sites. , 1998, International journal of oral and maxillofacial surgery.

[15]  T. Akazawa,et al.  Characterization of Differently Prepared Apatites by Adsorption Behavior of Albumin , 1996 .

[16]  M. Urist,et al.  Composites of bone morphogenetic protein (BMP) and type IV collagen, coral-derived coral hydroxyapatite, and tricalcium phosphate ceramics , 1996, International Orthopaedics.