The Micro-Ribonucleic Acid (miRNA) miR-206 Targets the Human Estrogen Receptor-α (ERα) and Represses ERα Messenger RNA and Protein Expression in Breast Cancer Cell Lines

Micro-RNAs are small noncoding RNAs, which diminish the stability and/or translation of mRNAs. This study examined whether miR-206, previously shown to be elevated in estrogen receptor (ER)α-negative breast cancer, regulates the expression of ERα. Two putative miR-206 sites, (hERα1 and hERα2), were found in silico within the 3′-untranslated region of human ERα mRNA. Transfection of MCF-7 cells with pre-miR-206 or 2′-O-methyl antagomiR-206 specifically decreased or increased, respectively, ERα mRNA levels. Overexpression of pre-miR-206 reduced ERα and β-actin protein levels, with no effect on ERβ, E-cadherin, or glyceraldehyde-3-phosphate dehydrogenase. Reporter constructs containing the hERα1 or hERα2 binding sites inserted into the 3′-untranslated region of the luciferase mRNA conferred a 1.6- and 2.2-fold repression of luciferase activity, respectively, in HeLa cells. Both miR-206 sites responded accordingly to exogenous hsa-pre-miR-206 and 2′-O-methyl antagomiR-206, and both sites were rendered inactiv...

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