Rapid fabrication of poly(dimethylsiloxane)-based microchip capillary electrophoresis devices using CO2 laser ablation.

The use of CO(2) laser ablation for the patterning of capillary electrophoresis (CE) microchannels in poly(dimethylsiloxane)(PDMS) is described. Low-cost polymer devices were produced using a relatively inexpensive CO(2) laser system that facilitated rapid patterning and ablation of microchannels. Device designs were created using a commercially available software package. The effects of PDMS thickness, laser focusing, power, and speed on the resulting channel dimensions were investigated. Using optimized settings, the smallest channels that could be produced averaged 33 microm in depth (11.1% RSD, N= 6) and 110 microm in width (5.7% RSD, N= 6). The use of a PDMS substrate allowed reversible sealing of microchip components at room temperature without the need for cleanroom facilities. Using a layer of pre-cured polymer, devices were designed, ablated, and assembled within minutes. The final devices were used for microchip CE separation and detection of the fluorescently labeled neurotransmitters aspartate and glutamate.

[1]  P. Yager,et al.  A rapid diffusion immunoassay in a T-sensor , 2001, Nature Biotechnology.

[2]  Holger Becker,et al.  Fabrication of plastic microchips by hot embossing. , 2002, Lab on a chip.

[3]  Ford,et al.  Polymeric microelectromechanical systems , 2000, Analytical chemistry.

[4]  Fahima Ouchen,et al.  An integrated solid‐phase extraction system for sub‐picomolar detection , 2002, Electrophoresis.

[5]  Anil K Deisingh.  MEMS technology in analytical chemistry. , 2003, The Analyst.

[6]  Richard A Mathies,et al.  Microfluidic devices for DNA sequencing: sample preparation and electrophoretic analysis. , 2003, Current opinion in biotechnology.

[7]  Dermot Diamond,et al.  CO2 laser microfabrication of an integrated polymer microfluidic manifold for the determination of phosphorus. , 2003, Lab on a chip.

[8]  G. Whitesides,et al.  Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane). , 1998, Analytical chemistry.

[9]  P. Grodzinski,et al.  Microfabricated polycarbonate CE devices for DNA analysis. , 2001, Analytical chemistry.

[10]  Shawn D. Llopis,et al.  Contact conductivity detection in poly(methyl methacrylate)-based microfluidic devices for analysis of mono- and polyanionic molecules. , 2002, Analytical chemistry.

[11]  A. D. Mello.  Focus: Plastic fantastic? , 2002 .

[12]  G. Whitesides,et al.  Poly(dimethylsiloxane) as a material for fabricating microfluidic devices. , 2002, Accounts of chemical research.

[13]  R. Givens,et al.  Naphthalene-2,3-dicarboxyaldehyde/cyanide ion: a rationally designed fluorogenic reagent for primary amines , 1987 .

[14]  M. Tarlov,et al.  Surface characterization of laser-ablated polymers used for microfluidics. , 2002, Analytical chemistry.

[15]  E. Verpoorte.  Microfluidic chips for clinical and forensic analysis , 2002, Electrophoresis.

[16]  Oliver Geschke,et al.  Microstructure fabrication with a CO2 laser system: characterization and fabrication of cavities produced by raster scanning of the laser beam. , 2003, Lab on a chip.

[17]  C. Henry,et al.  Dual-electrode electrochemical detection for poly(dimethylsiloxane)-fabricated capillary electrophoresis microchips. , 2000, Analytical chemistry.

[18]  Detlef Snakenborg,et al.  Microstructure fabrication with a CO2 laser system , 2004 .

[19]  N Gottschlich,et al.  Integrated microchip-device for the digestion, separation and postcolumn labeling of proteins and peptides. , 2000, Journal of chromatography. B, Biomedical sciences and applications.

[20]  S. Quake,et al.  Monolithic microfabricated valves and pumps by multilayer soft lithography. , 2000, Science.

[21]  G M Whitesides,et al.  Fabrication of topologically complex three-dimensional microfluidic systems in PDMS by rapid prototyping. , 2000, Analytical chemistry.

[22]  H. Girault,et al.  Photomodification of polymer microchannels induced by static and dynamic excimer ablation: effect on the electroosmotic flow. , 2001, Analytical chemistry.

[23]  Jin Ho Kim,et al.  Surface modification of poly(dimethylsiloxane) microchannels , 2003, Electrophoresis.