Life in ice: implications to astrobiology

During previous research expeditions to Siberia, Alaska and Antarctica, it was observed that glaciers and ice wedges contained bacterial cells that became motile as soon as the ice melted. This phenomenon of live bacteria in ice was first documented for microbes in ancient ice cores from Vostok, Antarctica. The first validly published species of Pleistocene bacteria alive on Earth today was Carnobacterium pleistocenium. This extremophile had remained for 32,000 years, encased in ice recently exposed in the Fox Tunnel of Alaska. These frozen bacteria began to swim as soon as the ice was thawed. Dark field microscopy studies revealed that large numbers of bacteria exhibited motility as soon as glacial ice was melted during our recent Expeditions to Alaska and Antarctica led to the conclusion that microbial life in ice was not a rare phenomenon. The ability of bacteria to remain alive while frozen in ice for long periods of time is of great significance to Astrobiology. In this paper, we describe the recent observations and advance the hypothesis that life in ice provides valuable clues to how we can more easily search for evidence of life on the Polar Caps of Mars, comets and other icy bodies of our Solar System. It is suggested that cryopanspermia may have played a far more important role in Origin of Life on Earth and the distribution of Life throughout the Cosmos and than previously thought possible.

[1]  Ram Veerapaneni,et al.  Isolation of Microbes from Lake Vostok Accretion Ice , 2008, Applied and Environmental Microbiology.

[2]  Richard B. Hoover,et al.  Psychrophiles and astrobiology: microbial life of frozen worlds , 2003, Other Conferences.

[3]  P. Price,et al.  Microorganisms metabolizing on clay grains in 3-km-deep Greenland basal ice. , 2006, Astrobiology.

[4]  Richard B. Hoover,et al.  Anaerobic psychrophiles from Alaska, Antarctica, and Patagonia: implications to possible life on Mars and Europa , 2002, SPIE Optics + Photonics.

[5]  A. Mulyukin,et al.  The structural bases of long-term anabiosis in non-spore-forming bacteria , 2006 .

[6]  M. N. Poglazova,et al.  Antarctic ice sheet as a model in search of life on other planets , 1998 .

[7]  Irina N Mitskevich,et al.  Long-term conservation of viable microorganisms in the ice sheet of Central Antarctica , 1998, Optics & Photonics.

[8]  T. Vishnivetskaya,et al.  The deep cold biosphere: facts and hypothesis , 1997 .

[9]  C. Russell,et al.  Galileo magnetometer measurements: a stronger case for a subsurface ocean at Europa. , 2000, Science.

[10]  C. T. Russell,et al.  Induced magnetic fields as evidence for subsurface oceans in Europa and Callisto , 1998, Nature.

[11]  Richard B. Hoover,et al.  Comparative Results of Using Different Methods for Discovery of Microorganisms in very Ancient Layers of the Central Antarctic Glacier above the Lake Vostok , 2002 .

[12]  Richard B. Hoover,et al.  Diatoms on earth, comets, europa and in interstellar space , 1986 .

[13]  Charles A. Hibbitts,et al.  Hydrated Salt Minerals on Ganymede's Surface: Evidence of an Ocean Below , 2001, Science.

[14]  D. Gilichinsky,et al.  Terrestrial Permafrost Models and Analogues of Martian Habitats and Inhabitants , 2009 .

[15]  T. D’elia,et al.  Isolation of fungi from Lake Vostok accretion ice , 2009, Mycologia.

[16]  J. B. Dalton,et al.  Near-infrared detection of potential evidence for microscopic organisms on Europa. , 2003, Astrobiology.

[17]  Richard B. Hoover,et al.  Significance to Astrobiology of Micro-Organisms in Permafrost and Ice , 2001 .

[18]  K. Nealson,et al.  Reproduction and metabolism at - 10 degrees C of bacteria isolated from Siberian permafrost. , 2003, Environmental microbiology.

[19]  V. Miteva,et al.  Herminiimonas glaciei sp. nov., a novel ultramicrobacterium from 3042 m deep Greenland glacial ice. , 2009, International journal of systematic and evolutionary microbiology.

[20]  John G. Duman,et al.  Thermal Hysteresis Protein Activity in Bacteria, Fungi, and Phylogenetically Diverse Plants , 1993 .

[21]  C. McKay,et al.  The possible origin and persistence of life on Enceladus and detection of biomarkers in the plume. , 2008, Astrobiology.

[22]  D. Gilichinsky,et al.  Micro-organisms and biomarkers in permafrost , 2001 .

[23]  P. Price.  A habitat for psychrophiles in deep Antarctic ice. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[24]  Richard B. Hoover,et al.  Trichococcus patagoniensis sp. nov., a facultative anaerobe that grows at −5 °C, isolated from penguin guano in Chilean Patagonia , 2006 .

[25]  S. Hensley,et al.  Titan's Rotation Reveals an Internal Ocean and Changing Zonal Winds , 2008, Science.

[26]  C. Chyba,et al.  Possible ecosystems and the search for life on Europa. , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[27]  V. K. Chistiakov,et al.  International Effort Helps Decipher Mysteries of Paleoclimate from Antarctic Ice Cores , 1995 .

[28]  Richard B. Hoover,et al.  Microorganisms and unicellular algae in the ice sheet of Antarctica , 1999, Optics & Photonics.

[29]  D. Gilichinsky.  Permafrost model of extraterrestrial habitat , 2002 .

[30]  E. Pierazzo,et al.  Thickness of a Europan Ice Shell from Impact Crater Simulations , 2001, Science.

[31]  G. E. Fogg,et al.  The Biology of Polar Regions , 2008 .

[32]  Richard B. Hoover,et al.  Environmental scanning electron microscopy investigations of ancient microorganisms from deep ice above Lake Vostok , 1999, Optics & Photonics.

[33]  Richard B. Hoover,et al.  Growth of the facultative anaerobes from Antarctica, Alaska, and Patagonia at low temperatures , 2004, SPIE Optics + Photonics.

[34]  A. I. Tsapin,et al.  Limnological conditions in Subglacial Lake Vostok, Antarctica , 2006 .

[35]  Richard B. Hoover,et al.  Microbial extremophiles from the 2008 Schirmacher Oasis Expedition: preliminary results , 2008, Optical Engineering + Applications.

[36]  S. Kieffer,et al.  Unified model of tectonics and heat transport in a frigid Enceladus , 2007, Proceedings of the National Academy of Sciences.

[37]  Mutsuo Ichinomiya,et al.  Structure of the summer under fast ice microbial community near Syowa Station, eastern Antarctica , 2007, Polar Biology.

[38]  J. Raymond,et al.  Release of an ice-active substance by Antarctic sea ice diatoms , 2004, Polar Biology.

[39]  Elena V. Pikuta,et al.  Cloning of the 16S ribosomal RNA gene of a psychrophilic bacterium from the Alaskan Fox Permafrost Tunnel , 2002, SPIE Optics + Photonics.

[40]  J. D. Bernal,et al.  “The Origins of Life” , 1957, Nature.

[41]  D. Gilichinsky,et al.  Clostridium tagluense sp. nov., a psychrotolerant, anaerobic, spore-forming bacterium from permafrost. , 2009, International journal of systematic and evolutionary microbiology.

[42]  M. K. Chattopadhyay.  Antifreeze proteins of bacteria , 2007 .

[43]  G. Neukum,et al.  Cassini Observes the Active South Pole of Enceladus , 2006, Science.

[44]  Andrew Pohorille,et al.  The NASA Astrobiology Roadmap. , 2008, Astrobiology.

[45]  R. J. Heckly.  Preservation of bacteria by lyophilization. , 1961, Advances in applied microbiology.

[46]  Charles V. Rice,et al.  Magnetic resonance tells microbiology where to go; bacterial teichoic acid protects liquid water at sub-zero temperatures , 2008, Optical Engineering + Applications.

[47]  M. A. Petrova,et al.  Cryoprotective properties of water in the Earth cryolithosphere and its role in exobiology , 1993, Origins of life and evolution of the biosphere.

[48]  D. Karl,et al.  Microorganisms in the accreted ice of Lake Vostok, Antarctica. , 1999, Science.

[49]  E. Mosley‐Thompson,et al.  Isolation of bacteria and 16S rDNAs from Lake Vostok accretion ice. , 2001, Environmental microbiology.

[50]  R. Dimmick,et al.  Studies on Survival of Bacteria: Rhythmic Response of Microorganisms to Freeze-Drying Additives , 1967, Applied microbiology.

[51]  V. Sorokovikov,et al.  Redistribution of soluble components during ice segregation in freezing ground , 2001 .

[52]  Damien Marsic,et al.  Carnobacterium pleistocenium sp. nov., a novel psychrotolerant, facultative anaerobe isolated from permafrost of the Fox Tunnel in Alaska. , 2005, International journal of systematic and evolutionary microbiology.

[53]  Charles V. Rice,et al.  Cryoprotection from bacterial teichoic acid , 2009, Optical Engineering + Applications.

[54]  P. Price,et al.  Microbial life in glacial ice and implications for a cold origin of life. , 2007, FEMS microbiology ecology.

[55]  Donald E. Wohlschlag,et al.  Freezing Resistance in Some Antarctic Fishes , 1969, Science.

[56]  D. Gilichinsky,et al.  Long-term preservation of microbial ecosystems in permafrost. , 1992, Advances in space research : the official journal of the Committee on Space Research.

[57]  V. Miteva,et al.  Rhodoglobus vestalii gen. nov., sp. nov., a novel psychrophilic organism isolated from an Antarctic Dry Valley lake. , 2003, International journal of systematic and evolutionary microbiology.

[58]  G. Pettengill,et al.  Observations of the north polar region of Mars from the Mars orbiter laser altimeter. , 1998, Science.

[59]  N. S. Duxbury,et al.  Super-long anabiosis of ancient microorganisms in ice and terrestrial models for development of methods to search for life on Mars, Europa and other planetary bodies , 2006 .