Smart 3D‐fish: Automation of distance analysis in nuclei of interphase cells by image processing

Detection of fluorescent probes by fluorescence in situ hybridization in cells with preserved three‐dimensional nuclear structures (3D‐FISH) is useful for studying the organization of chromatin and localization of genes in interphase nuclei. Fast and reliable measurements of the relative positioning of fluorescent spots specific to subchromosomal regions and genes would improve understanding of cell structure and function.

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