Novel collagen sponge reinforced with polyglycolic acid fiber produces robust, normal hair in murine hair reconstitution model.

The hair reconstitution assay is a useful system for studying cell-cell and epithelial-mesenchymal interaction. The current method consists of transplantation of both epidermal and dermal cells, using a silicone chamber placed on an athymic nude mouse. However, because of leakage and tilting of the grafted cells, the rate and area of hair growth vary depending on the chamber. We modified this method by using a collagen sponge as a scaffold and compared two types of collagen sponges, each having different tensile strengths. A conventional collagen sponge disturbed normal hair follicle formation; in contrast, a collagen sponge containing polyglycolic acid (PGA) fiber supported proper restructuring of skin and hair follicles. These data suggested the usefulness of PGA fiber-containing collagen sponges for hair reconstitution in research and clinical applications.

[1]  Yann Barrandon,et al.  Morphogenesis and Renewal of Hair Follicles from Adult Multipotent Stem Cells , 2001, Cell.

[2]  Y. Barrandon,et al.  Segregation of keratinocyte colony-forming cells in the bulge of the rat vibrissa. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[3]  T. Sun,et al.  Label-retaining cells reside in the bulge area of pilosebaceous unit: Implications for follicular stem cells, hair cycle, and skin carcinogenesis , 1990, Cell.

[4]  E. Fuchs,et al.  Stem cells in the skin: waste not, Wnt not. , 2003, Genes & development.

[5]  Y. Tabata,et al.  Fabrication and biocompatibility of collagen sponge reinforced with poly(glycolic acid) fiber. , 2003, Tissue engineering.

[6]  S. Boyce,et al.  Burn wound closure with cultured autologous keratinocytes and fibroblasts attached to a collagen-glycosaminoglycan substrate. , 1989, JAMA.

[7]  R. Burgeson,et al.  Selective activation of the versican promoter by epithelial- mesenchymal interactions during hair follicle development. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[8]  I. Yannas,et al.  Design of an artificial skin. I. Basic design principles. , 1980, Journal of biomedical materials research.

[9]  Yasuhiko Tabata,et al.  Polyhydroxyalkanonate derivatives in current clinical applications and trials. , 2003, Advanced drug delivery reviews.

[10]  S. Yuspa,et al.  Reconstitution of hair follicle development in vivo: determination of follicle formation, hair growth, and hair quality by dermal cells. , 1993, The Journal of investigative dermatology.

[11]  R. Bates,et al.  The growth of fetal mouse skin in cell culture and transplantation to F1 mice. , 1970, The Journal of investigative dermatology.

[12]  I. Mackenzie,et al.  Reformation of organized epidermal structure by transplantation of suspensions and cultures of epidermal and dermal cells , 2004, Cell and Tissue Research.

[13]  E Bell,et al.  Living tissue formed in vitro and accepted as skin-equivalent tissue of full thickness. , 1981, Science.

[14]  Yann Barrandon,et al.  Location of stem cells of human hair follicles by clonal analysis , 1994, Cell.

[15]  G. Dotto,et al.  Primary mouse keratinocyte cultures contain hair follicle progenitor cells with multiple differentiation potential. , 1997, The Journal of investigative dermatology.

[16]  S. Millar,et al.  Molecular mechanisms regulating hair follicle development. , 2002, The Journal of investigative dermatology.