Time, concentration, and pH parameters for the use of glutaraldehyde as a pulpotomy agent: an in vitro study.

The effects of time, concentration, and pH on glutaraldehyde ( GA) fixation were studied in vitro using 2 model systems: coIlagen-BSA gels as simulations of cell cytoplasm and enzyme activity in treated pulp tissue. The former system demonstrated that GA is most effective when buffered, and that its penetration is self-limiting. In general, concentration proved more a factor in the depth of penetration than did time. It was concluded from these experiments that buffering GA, increasing its concentration, and applying it for longer periods, all enhance the degree of fixation; only stronger solutions increase the depth of fixation. Practically, the data suggest that clinical treatment might involve using buffered glutaraldehyde -- either at 4% for 4 rain or 8% for 2 rain.

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