Titanium foam‐bioactive nanofiber hybrids for bone regeneration

We have reported previously a method to introduce bioactive nanofiber networks through self‐assembly into the pores of titanium alloy foams for bone repair. In this study we evaluate the in vitro colonization by mouse pre‐osteoblastic cells of these metal–peptide amphiphile hybrids containing phosphoserine residues and the RGDS epitope. The aim was to determine the effect of varying the RGDS epitope concentration within a given range, and confirm the ability for cells to infiltrate and survive within the nanofiber‐filled interconnected porosity of the hybrid material. We performed proliferation (DNA content) and differentiation assays (alkaline phosphatase and osteopontin expression) as well as SEM and confocal microscopy to evaluate cell colonization of the hybrids. At the RGDS epitope concentrations used in the nanofiber networks, all samples demonstrated significant cell migration into the hybrids, proliferation, and differentiation into osteoblastic lineage. Copyright © 2008 John Wiley & Sons, Ltd.

[1]  B. Geiger,et al.  Supramolecular crafting of cell adhesion. , 2007, Biomaterials.

[2]  Raghuvir Singh,et al.  Corrosion degradation and prevention by surface modification of biometallic materials , 2007, Journal of materials science. Materials in medicine.

[3]  M. Morra.  Biochemical modification of titanium surfaces: peptides and ECM proteins. , 2006, European cells & materials.

[4]  Samuel I Stupp,et al.  Presentation of RGDS epitopes on self-assembled nanofibers of branched peptide amphiphiles. , 2006, Biomacromolecules.

[5]  D. Dunand,et al.  Effect of initial preform porosity on solid-state foaming of titanium , 2006 .

[6]  Y. Gong,et al.  Investigation of MC3T3-E1 cell behavior on the surface of GRGDS-coupled chitosan. , 2006, Biomacromolecules.

[7]  P. Ducheyne,et al.  Nucleation and growth of calcium phosphate on amine-, carboxyl- and hydroxyl-silane self-assembled monolayers. , 2006, Biomaterials.

[8]  Maryam Tabrizian,et al.  Three-dimensional growth of differentiating MC3T3-E1 pre-osteoblasts on porous titanium scaffolds. , 2005, Biomaterials.

[9]  Andrés J. García.  Get a grip: integrins in cell-biomaterial interactions. , 2005, Biomaterials.

[10]  D. Dunand,et al.  High strength, low stiffness, porous NiTi with superelastic properties. , 2005, Acta Biomaterialia.

[11]  L. Brinson,et al.  A bioactive titanium foam scaffold for bone repair. , 2005, Acta biomaterialia.

[12]  Samuel I Stupp,et al.  Self-assembling peptide amphiphile nanofiber matrices for cell entrapment. , 2005, Acta biomaterialia.

[13]  S. Stupp,et al.  Presentation and recognition of biotin on nanofibers formed by branched peptide amphiphiles. , 2005, Nano letters.

[14]  S. Stupp,et al.  Coassembly of amphiphiles with opposite peptide polarities into nanofibers. , 2005, Journal of the American Chemical Society.

[15]  David C. Dunand,et al.  Processing of Titanium Foams , 2004 .

[16]  D. Dunand,et al.  Effect of thermal history on the superplastic expansion of argon-filled pores in titanium: Part I kinetics and microstructure , 2004 .

[17]  D. Dunand,et al.  Microstructure evolution during solid-state foaming of titanium , 2003 .

[18]  Samuel I Stupp,et al.  Colonization of organoapatite-titanium mesh by preosteoblastic cells. , 2003, Journal of biomedical materials research. Part A.

[19]  D. Dunand,et al.  Solid-state foaming of titanium by hydrogen-induced internal-stress superplasticity , 2003 .

[20]  Krista L. Niece,et al.  Self-assembly combining two bioactive peptide-amphiphile molecules into nanofibers by electrostatic attraction. , 2003, Journal of the American Chemical Society.

[21]  Samuel I Stupp,et al.  Self-assembling biomaterials: Liquid crystal phases of cholesteryl oligo(l-lactic acid) and their interactions with cells , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[22]  S. Stupp,et al.  Self-Assembling Biomaterials: l-Lysine-Dendron-Substituted Cholesteryl-(l-lactic acid)n̄ , 2002 .

[23]  S. Stupp,et al.  Cholesteryl-(L-lactic acid)n building blocks for self-assembling biomaterials , 2002 .

[24]  Giovanni Vozzi,et al.  Tissue Engineering: Methods for guiding cell disposition , 2001 .

[25]  Mamoru Mabuchi,et al.  Processing of biocompatible porous Ti and Mg , 2001 .

[26]  D. Dunand,et al.  Solid-state foaming of titanium by superplastic expansion of argon-filled pores , 2001 .

[27]  L G Griffith,et al.  Cell adhesion and motility depend on nanoscale RGD clustering. , 2000, Journal of cell science.

[28]  T. Bateman,et al.  Effect of nitinol implant porosity on cranial bone ingrowth and apposition after 6 weeks. , 1999, Journal of biomedical materials research.

[29]  P. Griss,et al.  Implant fixation by bone ingrowth. , 1999, The Journal of arthroplasty.

[30]  H. Rack,et al.  Titanium alloys in total joint replacement--a materials science perspective. , 1998, Biomaterials.

[31]  A A Campbell,et al.  Surface-induced mineralization: a new method for producing calcium phosphate coatings. , 1996, Journal of biomedical materials research.

[32]  J. Aubin,et al.  Osteoblast and chondroblast differentiation. , 1995, Bone.

[33]  B D Boyan,et al.  Effect of titanium surface roughness on proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63). , 1995, Journal of biomedical materials research.

[34]  J. Aubin,et al.  Simultaneous detection of multiple bone-related mRNAs and protein expression during osteoblast differentiation: polymerase chain reaction and immunocytochemical studies at the single cell level. , 1994, Developmental biology.

[35]  R M Pilliar,et al.  The optimum pore size for the fixation of porous-surfaced metal implants by the ingrowth of bone. , 1980, Clinical orthopaedics and related research.

[36]  A. Mata,et al.  Hybrid bone implants: self-assembly of peptide amphiphile nanofibers within porous titanium. , 2008, Biomaterials.

[37]  D. C. Dunandb,et al.  Effect of initial preform porosity on solid-state foaming of titanium , 2006 .

[38]  David L. Cochran,et al.  Mechanisms Involved in Osteoblast Response to Implant Surface Morphology , 2001 .

[39]  J. Ong,et al.  Hydroxyapatite and their use as coatings in dental implants: a review. , 2000, Critical reviews in biomedical engineering.

[40]  G. Stein,et al.  Concepts of osteoblast growth and differentiation: basis for modulation of bone cell development and tissue formation. , 1992, Critical reviews in oral biology and medicine : an official publication of the American Association of Oral Biologists.