Efficacy of selected disinfectants against mycobacteria and cryptosporidia.

We have tested the in-vitro efficacy of various disinfectants against clinical isolates of Mycobacterium tuberculosis, Mycobacterium avium-intracellulare, and against the protozoon Cryptosporidium parvum. The disinfectants were tested both with and without an organic load. 'Sactimed sinald' (a quaternary ammonium compound), 'Steris 20' (a peracetic acid compound) and 'Pentapon DC1' (a beta-ene compound) were all equally as effective as 'Cidex' (glutaraldehyde) against all the mycobacteria tested, as well as C. parvum. 'Virkon' (a per-oxygen compound) and 'Phoraid' (an iodine compound) were less effective than glutaraldehyde. 'Pentapon DHY' (a beta-ene compound) was ineffective against any of the organisms tested. We have also assessed a method of determining disinfectant efficacy using [35S]-methionine labelling of proteins, which appears promising, although it requires further evaluation.

[1]  J. Furr,et al.  Antimycobacterial activity of 'Virkon'. , 1993, The Journal of hospital infection.

[2]  B. van Klingeren,et al.  Comparative testing of disinfectants against Mycobacterium tuberculosis and Mycobacterium terrae in a quantitative suspension test. , 1987, The Journal of hospital infection.

[3]  K. Nelson,et al.  Transmission of tuberculosis by flexible fiberbronchoscopes. , 1983, The American review of respiratory disease.

[4]  G. Holmes,et al.  The significance of bacterial contamination of fibreoptic endoscopes. , 1980, The Journal of hospital infection.

[5]  S. Wright,et al.  Cleaning and disinfection of equipment for gastrointestinal flexible endoscopy: interim recommendations of a Working Party of the British Society of Gastroenterology. , 1989, Gut.

[6]  A. Woodcock,et al.  BRONCHOSCOPY AND INFECTION CONTROL , 1989, The Lancet.

[7]  J. M. Ascenzi,et al.  A more accurate method for measurement of tuberculocidal activity of disinfectants , 1987, Applied and environmental microbiology.

[8]  P. Chiodini,et al.  Characteristics and specificity of hybridoma antibodies against oocyst antigens of Cryptosporidium parvum from man , 1991, Parasite immunology.

[9]  F. Collins.  Bactericidal activity of alkaline glutaraldehyde solution against a number of atypical mycobacterial species. , 1986, The Journal of applied bacteriology.

[10]  D. Glass,et al.  The control of substances hazardous to health: Guidance for the initial assessment in hospitals , 1989 .

[11]  I. Smith,et al.  The labelling of microorganisms with [35S]methionine, thioATP or inorganic sulphate for taxonomic purposes , 1992 .

[12]  G. Ayliffe,et al.  Comparison of automated systems for the cleaning and disinfection of flexible fibreoptic endoscopes. , 1984, The Journal of hospital infection.

[13]  D. Norbäck,et al.  Skin and respiratory symptoms from exposure to alkaline glutaraldehyde in medical services. , 1988, Scandinavian journal of work, environment & health.

[14]  J. Fowler,et al.  Allergic contact dermatitis from glutaraldehyde exposure. , 1989, Journal of occupational medicine. : official publication of the Industrial Medical Association.