Experimental Demonstration of Localized Plasmonic Structured Illumination Microscopy.

Super-resolution imaging methods such as structured illumination microscopy and others have offered various compromises between resolution, imaging speed, and biocompatibility. Here we experimentally demonstrate a physical mechanism for super-resolution that offers advantages over existing technologies. Using finely structured, resonant, and controllable near-field excitation from localized surface plasmons in a planar nanoantenna array, we achieve wide-field surface imaging with resolution down to 75 nm while maintaining reasonable speed and compatibility with biological specimens.

[1]  D. Axelrod Total internal reflection fluorescence microscopy in cell biology. , 2003, Methods in enzymology.

[2]  Anne Sentenac,et al.  Structured illumination microscopy using unknown speckle patterns , 2012, Nature Photonics.

[3]  David A. Agard,et al.  Doubling the lateral resolution of wide-field fluorescence microscopy using structured illumination , 2000, Photonics West - Biomedical Optics.

[4]  G. Oster,et al.  Theoretical Interpretation of Moiré Patterns , 1964 .

[5]  John B. Pendry,et al.  Coherent Four-Fold Super-Resolution Imaging with Composite Photonic–Plasmonic Structured Illumination , 2015 .

[6]  Dimitrije Stamenović,et al.  Biomechanical imaging of cell stiffness and prestress with subcellular resolution , 2014, Biomechanics and modeling in mechanobiology.

[7]  Manish Saxena,et al.  Structured illumination microscopy , 2015 .

[8]  Numerical study of hyperlenses for three-dimensional imaging and lithography. , 2015, Optics express.

[9]  M. Gustafsson,et al.  Subdiffraction Multicolor Imaging of the Nuclear Periphery with 3D Structured Illumination Microscopy , 2008, Science.

[10]  W. Barnes,et al.  Surface plasmon subwavelength optics , 2003, Nature.

[11]  Yi Xiong,et al.  Far-field optical superlens. , 2007, Nano letters.

[12]  Zhaowei Liu,et al.  Wide field super-resolution surface imaging through plasmonic structured illumination microscopy. , 2014, Nano letters.

[13]  Michael J Rust,et al.  Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM) , 2006, Nature Methods.

[14]  Itaru Hamachi,et al.  Specific cell surface protein imaging by extended self-assembling fluorescent turn-on nanoprobes. , 2012, Journal of the American Chemical Society.

[15]  George C. Schatz,et al.  The Optical Properties of Metal Nanoparticles: The Influence of Size, Shape, and Dielectric Environment , 2003 .

[16]  M. Davidson,et al.  Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics , 2015, Science.

[17]  S. Simon,et al.  Imaging Constitutive Exocytosis with Total Internal Reflection Fluorescence Microscopy , 2000, The Journal of cell biology.

[18]  Zhaowei Liu,et al.  Plasmonic structured illumination microscopy. , 2010, Nano letters.

[19]  M. Gustafsson Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[20]  Bryant B. Chhun,et al.  Super-Resolution Video Microscopy of Live Cells by Structured Illumination , 2009, Nature Methods.

[21]  J. Lippincott-Schwartz,et al.  Imaging Intracellular Fluorescent Proteins at Nanometer Resolution , 2006, Science.

[22]  T. Wilson,et al.  Method of obtaining optical sectioning by using structured light in a conventional microscope. , 1997, Optics letters.

[23]  Zhaowei Liu,et al.  Localized plasmon assisted structured illumination microscopy for wide-field high-speed dispersion-independent super resolution imaging. , 2014, Nanoscale.

[24]  E. Meyhöfer,et al.  Single fungal kinesin motor molecules move processively along microtubules. , 2003, Biophysical journal.

[25]  Y. Zou,et al.  Antagonistic Functions of Dishevelleds Regulate Frizzled3 Endocytosis via Filopodia Tips in Wnt-Mediated Growth Cone Guidance , 2013, The Journal of Neuroscience.

[26]  S. Hell,et al.  STED microscopy with continuous wave beams , 2007, Nature Methods.

[27]  R. V. Van Duyne,et al.  Localized surface plasmon resonance spectroscopy and sensing. , 2007, Annual review of physical chemistry.

[28]  Dylan Lu,et al.  Hyperlenses and metalenses for far-field super-resolution imaging , 2012, Nature Communications.

[29]  Philipp J. Keller,et al.  Fast, high-contrast imaging of animal development with scanned light sheet–based structured-illumination microscopy , 2010, Nature Methods.

[30]  M. Gustafsson Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy , 2000, Journal of microscopy.