Detection of water quality parameters in Hangzhou Bay using a portable laser fluorometer.

A field, light-weight laser fluorometer based on the method of laser induced fluorescence was developed for water quality monitoring. The basic instrument configuration uses a high pulse repetition frequency microchip laser, a confocal reflective fluorescent probe and a broadband hyperspectral micro spectrometer; it weights only about 1.7 kg. Simultaneous estimates of three important water quality parameters, namely, chlorophyll a (chl-a), colored dissolved organic matter (CDOM), and total suspended matter (TSM) measured by the laser fluorometer were observed to agree well with those measured by traditional methods (0.27-0.84 μg L(-3) chl-a, R(2)=0.88; 0.104-0.295 m(-)(1) CDOM absorption, R(2)=0.90; and 59.8-994.9 mg L(-)(3) TSM, R(2)=0.86) in Hangzhou Bay water. Subsequently, distribution and characteristics of CDOM and chl-a laser fluorescence in Hangzhou Bay were analyzed, which will enhance our understanding of biogeochemical processes in this complex estuarine system at high-resolution, high-frequency and long-term scale.

[1]  Rainer Reuter,et al.  Hydrographic laser fluorosensing: Status and perspectives , 1997 .

[2]  H. Gordon,et al.  Remote Assessment of Ocean Color for Interpretation of Satellite Visible Imagery: A Review , 1983 .

[3]  Ryan Alexander,et al.  Identifying spatial structure in phytoplankton communities using multi‐wavelength fluorescence spectral data and principal component analysis , 2012 .

[4]  Pavel A. Salyuk,et al.  Comparative analysis of the chlorophyll A concentrations obtained by the laser-induced fluorescence method (LIF) and SeaWiFS , 2003, Other Conferences.

[5]  D. Savastru,et al.  LASER INDUCED FLUORESCENCE EFFICIENCY IN WATER QUALITY ASSESSMENT , 2010 .

[6]  Zhang Lin Optical characteristics of Colored Dissolved Organic Matter(CDOM) in Changjiang River Estuary and its adjacent sea areas , 2013 .

[7]  Yang Zuo-sheng,et al.  Transport and deposition of river sediment in the Changjiang estuary and adjacent continental shelf , 1985 .

[8]  C. Bernard,et al.  On the use of the FluoroProbe®, a phytoplankton quantification method based on fluorescence excitation spectra for large-scale surveys of lakes and reservoirs. , 2012, Water research.

[9]  A. Chekalyuk,et al.  Next generation Advanced Laser Fluorometry (ALF) for characterization of natural aquatic environments: new instruments. , 2013, Optics express.

[10]  Yan Bai,et al.  Seasonal variation of absorption spectral characteristics of CDOM and de-pigmented particles in East China Sea , 2008, Remote Sensing.

[11]  Lisa R. Moore,et al.  Determination of spectral absorption coefficients of particles, dissolved material and phytoplankton for discrete water samples , 2000 .

[12]  Victor V. Fadeev,et al.  ECOLOGICAL MONITORING IN THE CASPIAN SEA (MOUTH ZONE OF THE RIVER VOLGA) WITH A SHIPBOARD LASER SPECTROMETER , 2004 .

[13]  Heiko Balzter,et al.  Ultraviolet Fluorescence LiDAR (UFL) as a Measurement Tool for Water Quality Parameters in Turbid Lake Conditions , 2013, Remote. Sens..

[14]  E. Blaufuss,et al.  Measurements of the solar neutrino flux from Super-Kamiokande's first 300 days , 1998 .

[15]  V. Fedorov Studying Organic Species in Water by Laser Fluorescence Spectroscopy with a Source of Excitation in Mid-UV Range (266 nm) , 2005 .

[16]  M. Fingas,et al.  Review of the development of laser fluorosensors for oil spill application. , 2003, Marine pollution bulletin.

[17]  S. Lohrenz,et al.  Characterization of oil components from the Deepwater Horizon oil spill in the Gulf of Mexico using fluorescence EEM and PARAFAC techniques , 2013 .

[18]  Zhihua Mao,et al.  Analysis of the characters of chromophoric dissolved organic matter in water using laser induced fluorescence and spectral fluorescence signature , 2013, Optics & Photonics - Optical Engineering + Applications.

[19]  Robert F. Chen,et al.  Distribution of dissolved organic matter in the Pearl River Estuary, China , 2004 .

[20]  Collin S. Roesler,et al.  Fluorescence measured using the WETStar DOM fluorometer as a proxy for dissolved matter absorption , 2006 .

[21]  Sergey Babichenko,et al.  LASER REMOTE SENSING OF COASTAL AND TERRESTRIAL POLLUTION BY FLS-LIDAR , 2004 .

[22]  J. Milliman,et al.  Flux and fate of Yangtze River sediment delivered to the East China Sea , 2007 .

[23]  Sergey Babichenko,et al.  Airborne surveillance of water basins with hyperspectral FLS-LiDAR , 2010, Remote Sensing.

[24]  R. A. Velapoldi,et al.  Fluorescence standard reference material: quinine sulfate dihydrate. , 1981, Applied optics.

[25]  E. Peltzer,et al.  The use of in situ and airborne fluorescence measurements to determine UV absorption coefficients and DOC concentrations in surface waters , 1995 .

[26]  Robert F. Chen,et al.  The fluorescence of dissolved organic matter in the Mid-Atlantic Bight , 2002 .

[27]  F. H. Farmer,et al.  Laboratory analysis of techniques for remote sensing of estuarine parameters using laser excitation. , 1983, Applied optics.

[28]  H. Kuosa,et al.  Remote sensing of phytoplankton using laser-induced fluorescence , 1993 .

[29]  C. W. Wright,et al.  Short-pulse pump-and-probe technique for airborne laser assessment of Photosystem II photochemical characteristics , 2004, Photosynthesis Research.

[30]  M. Schaepman,et al.  Review of constituent retrieval in optically deep and complex waters from satellite imagery , 2012 .

[31]  S Babichenko,et al.  Fluorescent screening of phytoplankton and organic compounds in sea water. , 2000, Journal of environmental monitoring : JEM.

[32]  F. Muller‐Karger,et al.  Colored Dissolved Organic Matter in Tampa Bay, Florida , 2007 .

[33]  William G. Booty,et al.  Spectral absorption properties of dissolved and particulate matter in Lake Erie , 2008 .

[34]  Gary B. Collins,et al.  Vitro Determination of Chlorophyll a and Pheophytin a in Marine and Freshwater Algae by Fluorescence , 2013 .

[35]  Antonio Palucci,et al.  LIDAR CALIBRATION OF SATELLITE SENSED CDOM IN THE SOUTHERN OCEAN , 2006 .

[36]  D. Killinger,et al.  Development and initial calibration of a portable laser-induced fluorescence system used for in situ measurements of trace plastics and dissolved organic compounds in seawater and the Gulf of Mexico. , 2003, Applied optics.

[37]  Donald H. Bundy,et al.  Use of water Raman emission to correct airborne laser fluorosensor data for effects of water optical attenuation. , 1981, Applied optics.

[38]  N. Welschmeyer Fluorometric analysis of chlorophyll a in the presence of chlorophyll b and pheopigments , 1994 .

[39]  A. Chekalyuk,et al.  Advanced laser fluorometry of natural aquatic environments , 2008 .

[40]  J. Cloern Our evolving conceptual model of the coastal eutrophication problem , 2001 .

[41]  S. Babichenko Laser Remote Sensing of the European Marine Environment: LIF Technology and Applications , 2008 .

[42]  R. Reuter,et al.  Lidar fluorosensing of mineral oil spills on the sea surface. , 1990, Applied optics.

[43]  C. Mobley,et al.  Optical modeling of ocean waters: Is the case 1 - case 2 classification still useful? , 2004 .

[44]  Jean Verdebout,et al.  Determination of aquatic parameters by a time-resolved laser fluorosensor operating from a helicopter , 1992, Other Conferences.

[45]  R. Reuter,et al.  Fluorescent matter in the eastern Atlantic Ocean. Part 1: method of measurement and near-surface distribution , 1994 .

[46]  Jerome P Ferrance,et al.  A low-cost, low-power consumption, miniature laser-induced fluorescence system for DNA detection on a microfluidic device. , 2007, Clinics in laboratory medicine.

[47]  R. Conmy,et al.  Calibration and performance of a new in situ multi-channel fluorometer for measurement of colored dissolved organic matter in the ocean , 2004 .

[48]  D. Pan,et al.  Fluorescence measured using a field-portable laser fluorometer as a proxy for CDOM absorption , 2014 .

[49]  Antonio Palucci,et al.  ENVIRONMENTAL MONITORING BY LASER RADAR , 2011 .

[50]  D. Pan,et al.  Development of a portable laser-induced fluorescence system used for in situ measurements of dissolved organic matter , 2014 .

[51]  Rui Pitanga Marques da Silva,et al.  Metrological assessment of LIDAR signals in water , 2011 .

[52]  C. Stedmon,et al.  The optics of chromophoric dissolved organic matter (CDOM) in the Greenland Sea: An algorithm for differentiation between marine and terrestrially derived organic matter , 2001 .

[53]  D. Keith,et al.  Spatial and Temporal Distribution of Coloured Dissolved Organic Matter (CDOM) in Narragansett Bay, Rhode Island: Implications for Phytoplankton in Coastal Waters , 2002 .