论文信息 - Brain and blood extraction for immunostaining, protein, and RNA measurements after long-term two photon imaging in mice.

Brain and blood extraction for immunostaining, protein, and RNA measurements after long-term two photon imaging in mice.

This protocol describes a method to extract brain tissue and whole blood samples to perform immunostaining, protein extraction for ELISA, western blot, or RNA extraction for qPCR after long-term in vivo imaging. This protocol is in particular useful to process and maintain valuable tissue samples, allowing for a broad spectrum of analysis and techniques without compromising the quality of the samples.

Nancy Ruiz-Uribe | O. Bracko

[1] Yun-Kyoung Lee,et al. The isolation and molecular characterization of cerebral microvessels , 2019, Nature Protocols.

[2] B. Teferedegne,et al. A simple, inexpensive method for preparing cell lysates suitable for downstream reverse transcription quantitative PCR , 2014, Scientific Reports.

[3] S. Spijker,et al. Dissection of Rodent Brain Regions , 2011 .

[4] S. M. Dyer,et al. An overview of restraint and blood collection techniques in exotic pet practice. , 2008, The veterinary clinics of North America. Exotic animal practice.

[5] Robin Hull,et al. A good practice guide to the administration of substances and removal of blood, including routes and volumes , 2001, Journal of applied toxicology : JAT.

Brain and blood extraction for immunostaining, protein, and RNA measurements after long-term two photon imaging in mice﻿.

Brain and blood extraction for immunostaining, protein, and RNA measurements after long-term two photon imaging in mice.