Isolation and Evaluation of Antimicrobial Properties of Isolated Phytoconstituents of Fruits of Helicteres isora Linn.

Helicteres isora linn, an ayurvedic herb, is distributed widely in forest throughout India. Almost its all parts are used traditionally. The fruits are useful in diarrhoea, dysentery, wounds, ulcers, hemorrhages and diabetes. The fruit after collection, authentication and drying, was extracted with petroleum ether, chloroform, methanol and water using soxhlet extractor.The present study was aimed to isolate the constituents and evaluate the antimicrobial properties of isolated constituents of methanolic extract of fruits of Helicteres isora linn. Isolation of alkaloids, flavonoids and phenolic compounds was done by using HPTLC technique. The invitro antimicrobial activities of methanolic extract, isolated alkaloids, flavonoids and phenolic compounds were investigated against Escherichia coli, Pseudomonas aeruginosa, Salmonella abony and Staphylococcus aureus by cup-plate diffusion method.The methanolic extract showed activity against Escherichia coli, Pseudomonas aeruginosa, Salmonella abony and Staphylococcus aureus., isolated phenolic compounds against Escherichia coli, Pseudomonas aeruginosa and Salmonella abony., isolated flavonoids against Pseudomonas aeruginosa and Staphylococcus aureus., and isolated alkaloids against Escherichia coli and Staphylococcus aureus.Minimum inhibitory concentration was determined by liquid broth method. Minimum inhibitory concentration of methanolic extract against Pseudomonas aeruginosa was found to be 10µg/ml and that against Staphylococcus aureuswas found to