BACKGROUND AND AIM
We proposed a novel temperature-response collagen sol as submucosal injection agent for endoscopic resection (ER) using pepsin-solubilized collagen (PSC) and genipin (Ge) in a prior study. This study aims to evaluate usefulness and safety of the sol (PSC/Ge) in acute and chronic phase experiments using living animals.
METHODS
In experiment 1, we performed endoscopic submucosal dissection (ESD) for six pigs using normal saline (NS), sodium hyaluronate (SH), and PSC/Ge. We compared required amount of each agents per unit area and procedure time. In experiment 2, we created artificial ulcers with endoscopic mucosal resection (EMR) for five pigs using NS and PSC sol. We compared the artificial ulcer residual rate in 7 and 14 days after EMR, and the scarring rate in 14 days after EMR.
RESULTS
Required amount of agents per unit area for PSC/Ge (0.8±0.8 ml/cm2 ) and SH (1.1±0.8 ml/cm2 ) were significantly smaller than that for NS (1.8±0.7 ml/cm2 ). The total procedure time didn't have a statistical difference. The artificial ulcer residual rate were 47.3±0.7% for NS and 40.3±0.7% for PSC/Ge on day 7(p=0.51), and 15.0±0.1% for NS and 10.2±0.1% for PSC/Ge sol on day 14 (p=0.35). The scarring rate on day 14 was 10% for NS and 20% for PSC/Ge.
CONCLUSION
We demonstrated the feasibility of a novel temperature-response collagen gel as a submucosal injection agent for ER in the acute and chronic phase animal experiment.