Rosmarinus officinalis essential oil: antiproliferative, antioxidant and antibacterial activities

The aim of this work was to investigate and compare the antiproliferative, antioxidant and antibacterial activities of Rosmarinus officinalis essential oil, native to Pakistan. The essential oil content from the leaves of R. officinalis was 0.93 g 100g-1. The GC and GC-MS analysis revealed that the major components determined in R. officinalis essential oil were 1,8-cineol (38.5%), camphor (17.1%), α-pinene (12.3%), limonene (6.23%), camphene (6.00%) and linalool (5.70%). The antiproliferative activity was tested against two cancer (MCF-7 and LNCaP) and one fibroblast cell line (NIH-3T3) using the MTT assay, while, the antioxidant activity was evaluated by the reduction of 2, 2-diphenyl-1-picryl hydrazyl (DPPH) and measuring percent inhibition of peroxidation in linoleic acid system. The disc diffusion and modified resazurin microtitre-plate assays were used to evaluate the inhibition zones (IZ) and minimum inhibitory concentration (MIC) of R. officinalis essential oil, respectively. It is concluded from the results that Rosmarinus officinalis essential oil exhibited antiproliferative, antioxidant and antibacterial activities.

[1]  R. Mota,et al.  Antibacterial activity, chemical composition, and cytotoxicity of leaf’s essential oil from brazilian pepper tree (schinus terebinthifolius, raddi) , 2010, Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology].

[2]  R. Mota,et al.  Antibacterial activity, chemical composition, and cytotoxicity of leaf's essential oil from Brazilian pepper tree (Schinus terebinthifolius, Raddi) , 2010 .

[3]  Shayne C. Gad,et al.  Alternatives to In vivo Studies in Toxicology , 2009 .

[4]  Farid Chemat,et al.  Comparison of two isolation methods for essential oil from rosemary leaves: Hydrodistillation and microwave hydrodiffusion and gravity , 2009 .

[5]  Y. Zu,et al.  Antioxidative activity of Rosmarinus officinalis L. essential oil compared to its main components. , 2008, Food chemistry.

[6]  F. Anwar,et al.  Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations. , 2008, Food chemistry.

[7]  A. Figueiredo,et al.  Seasonal variation in essential oil composition, oil toxicity and the biological activity of solvent extracts of three South African Salvia species , 2008 .

[8]  N. Mimica-Dukić,et al.  Antimicrobial and antioxidant properties of rosemary and sage (Rosmarinus officinalis L. and Salvia officinalis L., Lamiaceae) essential oils. , 2007, Journal of agricultural and food chemistry.

[9]  L. Nahar,et al.  Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals , 2007, Methods.

[10]  F. Sukan,et al.  Antimicrobial activities of methanol extracts and essential oils of Rosmarinus officinalis, depending on location and seasonal variations , 2007 .

[11]  I. Rasooli,et al.  Chemical and biological characteristics of Cuminum cyminum and Rosmarinus officinalis essential oils , 2007 .

[12]  D. Runyoro,et al.  Composition and antimicrobial activity of the essential oils of three Satureja species growing in Tanzania , 2007 .

[13]  Shahid Iqbal,et al.  Antioxidant properties and components of some commercially available varieties of rice bran in Pakistan , 2005 .

[14]  E. Koschier,et al.  Labiate essential oils affecting host selection and acceptance of Thrips tabaci lindeman , 2003 .

[15]  Y. Yamaoka,et al.  Antimicrobial Activity of Essential Oils against Helicobacter pylori , 2003, Helicobacter.

[16]  N. Mimica-Dukić,et al.  Antimicrobial and Antioxidant Activities of Three Mentha Species Essential Oils , 2003, Planta medica.

[17]  F. Tomi,et al.  Chemical composition and antimicrobial activity of Rosmarinus officinalis L. oils from Sardinia and Corsica , 2002 .

[18]  Robert P. Adams,et al.  Identification of essential oil components by gas chromatography/quadrupole mass spectroscopy , 2001 .

[19]  E. Koenen.  Medicinal, poisonous and edible plants in Namibia , 2001 .

[20]  T. Mosmann.  Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. , 1983, Journal of immunological methods.

[21]  E. Bruck,et al.  National Committee for Clinical Laboratory Standards. , 1980, Pediatrics.

[22]  Y. Masada.  Analysis of essential oils by gas chromatography and mass spectrometry , 1976 .