Influence of phenylacetic acid on clonal propagation of Decalepis hamiltonii wight & ARN: An endangered shrub

SummaryWe have developed a highly efficient two-stage protocol for induction of multiple shoots from single node in vitro shoot tip explants of Decalepis hamiltonii. It was found that phenylacetic acid (PAA) had a synergistic effect on shoot multiplication when treated with N6-benzyladenine (BA). This protocol used PAA for both multiple shoot induction from nodal explants, elongation of primary shoots, and initiation of adventitious shoot formation from primary shoots. Murashige and Skoog medium containing BA (2.22–31.08 μM) and α-naphthaleneacetic acid (0.27–10.74 μM) or PAA (7.34–36.71 μM) was used to initiate shoot formation from nodal explants. The maximum number of shoots per culture was produced on a medium containing 31.08 μM BA and 14.68 μM PAA, while the longest shoot length and nodes were obtained on medium containing 22.2 μM BA and 14.68 μM PAA. Shoots subcultured on MS medium containing 22.2 μM BA and 14.68 μM PAA elongated along with secondary shoot formation. The shoots were rooted on medium containing 9.7 μM indole-3-butyric acid. The plantlets were acclimatized in soil with an 80–90% survival rate under field conditions.

[1]  A. Sarrafi,et al.  Genetic control of organogenesis in cotyledons of sunflower (Helianthus annuus) , 1997, Plant Cell, Tissue and Organ Culture.

[2]  S. Kothari,et al.  Phenylacetic acid induced organogenesis in cultured leaf segments of Dianthus chinensis , 1996, Plant Cell Reports.

[3]  G. Sim,et al.  Synergistic effect of ethylene inhibitors and putrescine on shoot regeneration from hypocotyl explants of Chinese radish (Raphanus sativus L. var. longipinnatus Bailey) in vitro , 1996, Plant Cell Reports.

[4]  P. Saxena,et al.  Phenylacetic acid-induced somatic embryogenesis in cultured hypocotyl explants of geranium (Pelargonium x hortorum Bailey) , 1991, Plant Cell Reports.

[5]  D. Morris,et al.  Promotion of elongation and acid invertase activity in Phaseolus vulgaris L. internode segments by phenylacetic acid , 1990, Plant Growth Regulation.

[6]  Gokare A. Ravishankar,et al.  In vitro rooting of Decalepis hamiltonii Wight & Arn., an endangered shrub, by auxins and root-promoting agents , 2001 .

[7]  H. Bais,et al.  Optimization of media constituents for shoot regeneration from leaf callus cultures of Decalepis hamiltonii Wight. & Arn. , 2000 .

[8]  A. Jain,et al.  Phenylacetic acid improves bud elongation and in vitro plant regeneration efficiency in Capsicum annuum L. , 1999, Plant Cell Reports.

[9]  G. Hahne,et al.  Plant regeneration at high frequency from mature sunflower cotyledons , 1991 .

[10]  F. Wightman,et al.  Auxins of non‐flowering plants. I. Occurrence of 3‐indoleacetic acid and phenylacetic acid in vegetative and fertile fronds of the ostrich fern (Matteucia struthiopteris) , 1986 .

[11]  L. G. Nickell.  Plant Growth Regulators , 1982 .

[12]  K. Thimann,et al.  Hormonal factors controlling the initiation and development of lateral roots , 1980 .

[13]  A. Wheeler.  Auxin-like Growth Activity of Phenylacetonitrile , 1977 .

[14]  H. Abé,et al.  Isolation of Phenylacetic Acid and Its p-Hydroxy Derivative as Auxin-like Substances from Undaria pinnatifida , 1974 .

[15]  T. Koizumi,et al.  Studies on Plant Growth Regulators. I., Isolation of Indole-3-acetamide, 2-Phenylacetamide, and Indole-3-carboxaldehyde from Etiolated Seedlings of Phaseolus , 1967 .

[16]  F. Skoog,et al.  A revised medium for the growth and bioassay with tobacco tissue culture , 1962 .