Fermentative Bioethanol Production Using Enzymatically Hydrolysed Saccharina latissima

The increased demand for machinery and transport has led to an overwhelming increase in the use of fossil fuels in the last century. Concerning the economic and environmental concern, macroalgae with high fermentable polysaccharide content (mainly mannitol, cellulose and laminarin), can serve as an excellent alternative to food crops for bioethanol production, a renewable liquid fuel. In this study, Saccharina latissima, a brown macroalgae readily available on the Norwegian coast was used as the carbohydrate source for the fermentative production of bioethanol. The macroalgae harvested was found to contain 31.31 ± 1.73 g of reducing sugars per 100 g of dry Saccharina latissima upon enzymatic hydrolysis. The subsequent fermentation with Saccharomyces cerevisiae produced an ethanol yield of 0.42 g of ethanol per g of reducing sugar, resulting in a fermentation efficiency of 84% as compared to the theoretical maximum. Using these results, an evaluation of the fermentation process has demonstrated that the brown macroalgae Saccharina latissima could become a viable bioethanol source in the future.

[1]  C. T. Chong,et al.  Production of liquid biofuels (biodiesel and bioethanol) from brown marine macroalgae Padina tetrastromatica , 2017 .

[2]  L. C. Meher,et al.  Technical aspects of biodiesel production by transesterification—a review , 2006 .

[3]  Christine Nicole S. Santos,et al.  An Engineered Microbial Platform for Direct Biofuel Production from Brown Macroalgae , 2012, Science.

[4]  F. Bux,et al.  Biodiesel from microalgae: A critical evaluation from laboratory to large scale production , 2013 .

[5]  Keat Teong Lee,et al.  A visionary and conceptual macroalgae-based third-generation bioethanol (TGB) biorefinery in Sabah, Malaysia as an underlay for renewable and sustainable development , 2010 .

[6]  S. Horn,et al.  Enzymatic saccharification of brown seaweed for production of fermentable sugars. , 2016, Bioresource technology.

[7]  M. Stanley,et al.  The seasonal variation in the chemical composition of the kelp species Laminaria digitata, Laminaria hyperborea, Saccharina latissima and Alaria esculenta , 2015, Journal of Applied Phycology.

[8]  Xianhua Liu,et al.  Two-stage hydrolysis of invasive algal feedstock for ethanol fermentation. , 2011, Journal of integrative plant biology.

[9]  I. Peinado,et al.  Chemical composition, antioxidant activity and sensory evaluation of five different species of brown edible seaweeds , 2014 .

[10]  G. Knothe.  Analyzing biodiesel: standards and other methods , 2006 .

[11]  Christine Nicole S. Santos,et al.  Efficient ethanol production from brown macroalgae sugars by a synthetic yeast platform , 2013, Nature.

[12]  G. Luo,et al.  Macroalgae for biofuels production: progress and perspectives. , 2015 .

[13]  Rishi Gupta,et al.  Bioethanol production from Gracilaria verrucosa, a red alga, in a biorefinery approach. , 2013, Bioresource technology.

[14]  Ho Nam Chang,et al.  Ethanol production from marine algal hydrolysates using Escherichia coli KO11. , 2011, Bioresource technology.

[15]  Yuqing Su,et al.  An overview of biofuels policies and industrialization in the major biofuel producing countries , 2015 .

[16]  Sung-Koo Kim,et al.  Optimization of saccharification and ethanol production by simultaneous saccharification and fermentation (SSF) from seaweed, Saccharina japonica , 2011, Bioprocess and Biosystems Engineering.

[17]  Yong-Ki Hong,et al.  Detoxification of acidic catalyzed hydrolysate of Kappaphycus alvarezii (cottonii) , 2011, Bioprocess and Biosystems Engineering.

[18]  G. L. Miller.  Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar , 1959 .

[19]  S. Horn,et al.  The role of alginate lyases in the enzymatic saccharification of brown macroalgae, Macrocystis pyrifera and Saccharina latissima , 2017 .

[20]  R. C. Kuhad,et al.  Fed batch enzymatic saccharification of newspaper cellulosics improves the sugar content in the hydrolysates and eventually the ethanol fermentation by Saccharomyces cerevisiae , 2010 .

[21]  P. Maiti,et al.  Kappaphycus alvarezii as a source of bioethanol. , 2012, Bioresource technology.

[22]  A. Magrí,et al.  Spectrophotometric micro-method for the determination of ethanol in commercial beverages , 1997 .

[23]  S. Horn,et al.  Ethanol production from seaweed extract , 2000, Journal of Industrial Microbiology and Biotechnology.

[24]  S. Horn,et al.  Production of ethanol from mannitol by Zymobacter palmae , 2000, Journal of Industrial Microbiology and Biotechnology.

[25]  A. N. Boyce,et al.  Biodiesel Fuel Production from Algae as Renewable Energy , 2008 .

[26]  S. Olsen,et al.  A critical review of biochemical conversion, sustainability and life cycle assessment of algal biofuels , 2011 .

[27]  Rishi Gupta,et al.  Separate hydrolysis and fermentation (SHF) of Prosopis juliflora, a woody substrate, for the production of cellulosic ethanol by Saccharomyces cerevisiae and Pichia stipitis-NCIM 3498. , 2009, Bioresource technology.

[28]  J. Marchetti,et al.  Possible methods for biodiesel production , 2007 .

[29]  D. Sahoo,et al.  Using algae for carbon dioxide capture and bio-fuel production to combat climate change , 2012 .

[30]  S. Kraan,et al.  Mass-cultivation of carbohydrate rich macroalgae, a possible solution for sustainable biofuel production , 2010, Mitigation and Adaptation Strategies for Global Change.

[31]  Kiyohiko Nakasaki,et al.  Production of high concentrations of bioethanol from seaweeds that contain easily hydrolyzable polysaccharides , 2011 .

[32]  Y. J. Kim,et al.  Use of Gelidium amansii as a promising resource for bioethanol: a practical approach for continuous dilute-acid hydrolysis and fermentation. , 2012, Bioresource technology.

[33]  F. Smith,et al.  COLORIMETRIC METHOD FOR DETER-MINATION OF SUGAR AND RELATED SUBSTANCE , 1956 .

[34]  H. Oh,et al.  Comparison of several methods for effective lipid extraction from microalgae. , 2010, Bioresource technology.

[35]  L. Xia,et al.  Enzymatic hydrolysis of corncob and ethanol production from cellulosic hydrolysate , 2007 .

[36]  D. Herák,et al.  Behaviour of different moisture contents of Jatropha curcas L. seeds under compression loading. , 2018 .

[37]  Woon Yong Choi,et al.  Repeated-batch operation of surface-aerated fermentor for bioethanol production from the hydrolysate of seaweed Sargassum sagamianum. , 2011, Journal of microbiology and biotechnology.

[38]  Rishi Gupta,et al.  Bioethanol production from Lantana camara (red sage): Pretreatment, saccharification and fermentation. , 2010, Bioresource technology.

[39]  D. Tuli,et al.  Pilot scale study on steam explosion and mass balance for higher sugar recovery from rice straw. , 2015, Bioresource technology.

[40]  Wen Tong Chong,et al.  A review on potential enzymatic reaction for biofuel production from algae , 2014 .

[41]  Jiuping Xu,et al.  Innovative technological paradigm-based approach towards biofuel feedstock , 2017 .

[42]  Iain S. Donnison,et al.  Fermentation study on Saccharina latissima for bioethanol production considering variable pre-treatments , 2009, Journal of Applied Phycology.

[43]  Leena P Devendra,et al.  Evaluation of hydrotropic pretreatment on lignocellulosic biomass. , 2016, Bioresource technology.

[44]  H. Pingaud,et al.  Metabolic transition step from ethanol consumption to sugar/ethanol , 2004, Biotechnology Letters.

[45]  N. Trivedi,et al.  Enzymatic hydrolysis and production of bioethanol from common macrophytic green alga Ulva fasciata Delile. , 2013, Bioresource technology.

[46]  I. Donnison,et al.  Seasonal variation in Laminaria digitata and its impact on biochemical conversion routes to biofuels. , 2011, Bioresource technology.