A new method to quantify behavioral attention to a stimulus object in a modified open-field

A new method was developed to measure locomotion and attention to a stimulus object in a typical open-field environment using a video image-analyzer system. A computer-generated central zone (CZ) composing 1/9 of the open-field floor area was monitored independently from the rest of the area. Intermittently, a 4 x 4 x 2 cm object was placed in the CZ. In 10 min test sessions, the presence of the object repeatedly and consistently increased the animal's time spent in the CZ as compared with tests when the object was absent. The presence of the object, however, did not increase either the number of entries to the CZ or overall locomotor activity. The object reliably elicited an investigatory response when the animal enter the CZ but otherwise was behaviorally neutral. Thus, by incorporating a stimulus object in a conventional open-field test environment, the present methodology extends the open-field test beyond the measurement of overall spontaneous activity to include an assessment of behavioral processes linked to attention mechanisms.