Sensitive capillary electrophoresis microchip determination of trinitroaromatic explosives in nonaqueous electrolyte following solid phase extraction

Abstract A capillary electrophoresis (CE) microchip is utilized for the sensitive separation and detection of three trinitroaromatic explosives: 1,3,5-trinitrotoluene (TNT), 1,3,5-trinitrobenzene (TNB) and 2,4,6-trinitrophenyl- N -methylnitramine (tetryl), in the presence of 10 other explosives and explosive derivatives in nonaqueous electrolyte (acetonitrile/methanol 87.5/12.5 (v/v), 2.5 mM NaOH, 1 mM sodium dodecyl sulfate (SDS)). The chemical reaction of bases, e.g. hydroxide or methoxide ions, with trinitroaromatic compounds forms red colored derivatives that can be easily detected using a green light emitting diode (LED) on the microchip. Two surfactants bearing opposite charge, cetyltrimethylammonium bromide (CTAB) and SDS are compared with respect to their effect on separation times, detection limits and resolving powers for separating these explosives. All microchip separations were achieved in

[1]  Qin Lu,et al.  Radionuclide and metal ion detection on a capillary electrophoresis microchip using LED absorbance detection , 2001 .

[2]  I. Liška.  Fifty years of solid-phase extraction in water analysis--historical development and overview. , 2000, Journal of chromatography. A.

[3]  J Wang,et al.  Micromachined electrophoresis chips with thick-film electrochemical detectors. , 1999, Analytical chemistry.

[4]  J. Jorgenson,et al.  Capillary zone electrophoresis of neutral organic molecules by solvophobic association with tetraalkylammonium ion , 1986 .

[5]  J. Michael Ramsey,et al.  Solid phase extraction on microfluidic devices , 2000 .

[6]  Hilmi,et al.  Electrochemical detectors prepared by electroless deposition for microfabricated electrophoresis chips , 2000, Analytical chemistry.

[7]  R. M. Seifar,et al.  Mechanism of Electrokinetic Separations of Hydrophobic Compounds with Sodium Dodecyl Sulfate in Acetonitrile−Water Mixtures , 1997 .

[8]  F. Matysik.  Potentialities of electrochemical detection in conjunction with non-aqueous capillary electrophoresis , 1998 .

[9]  M. Strauss.  Anionic sigma complexes , 1970 .

[10]  J. Miller,et al.  Separation of polycyclic aromatic hydrocarbons by nonaqueous capillary electrophoresis using charge-transfer complexation with planar organic cations. , 1997, Analytical chemistry.

[11]  P. Buck.  Reactions of Aromatic Nitro Compounds with Bases , 1969 .

[12]  R. T. Medary.  Inexpensive, rapid field screening test for 2,4,6-trinitrotoluene in soil , 1992 .

[13]  J. Meisenheimer.  Ueber Reactionen aromatischer Nitrokörper , 2022 .

[14]  Hossein Salimi-Moosavi,et al.  Electroosmotic Pumping of Organic Solvents and Reagents in Microfabricated Reactor Chips , 1997 .

[15]  Bailey,et al.  Separation and detection of explosives on a microchip using micellar electrokinetic chromatography and indirect laser-induced fluorescence , 2000, Analytical chemistry.

[16]  Ann B. Strong,et al.  Comparison of solid phase extraction with salting-out solvent extraction for preconcentration of nitroaromatic and nitramine explosives from water , 1994 .

[17]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[18]  J. Foley,et al.  Separation of very hydrophobic compounds by hydrophobic interaction electrokinetic chromatography , 1994 .

[19]  S. Jacobson,et al.  Solvent-programmed microchip open-channel electrochromatography. , 1998, Analytical chemistry.

[20]  Qin Lu,et al.  Microfabricated capillary electrophoresis sensor for uranium (VI) , 2001 .

[21]  C. F. Bernasconi,et al.  Intermediates in nucleophilic aromatic substitution. II. Temperature-jump study of the interaction of 1,3,5-trinitrobenzene with aliphatic amines in 10% dioxane-90% water. Concurrent nucleophilic attack on the aromatic carbon and on the nitro group , 1970 .

[22]  R. Oleschuk,et al.  Trapping of bead-based reagents within microfluidic systems: on-chip solid-phase extraction and electrochromatography , 2000, Analytical chemistry.

[23]  Colin A. Fyfe,et al.  Flow nuclear magnetic resonance investigation of the transient and stable species formed by the attack of alkoxide ions on 2,4,6-trinitrotoluene , 1976 .

[24]  G. Collins,et al.  Microchip separations of transition metal ions via LED absorbance detection of their PAR complexes. , 2001, The Analyst.

[25]  J. Dorsey,et al.  Behavior and use of nonaqueous media without supporting electrolyte in capillary electrophoresis and capillary electrochromatography. , 1997, Analytical chemistry.

[26]  J H Luong,et al.  Nonaqueous capillary electrophoresis equipped with amperometric detection for analysis of chlorinated phenolic compounds. , 1999, Journal of chromatography. A.