Influence of ammonium and nitrate nutrition on the pyridine and adenine nucleotides of soybean and sunflower.

Total pyridine nucleotide concentration of root tissue for young soybean (Glycine max var. Bansei) and sunflower (Helianthus annuus L. var. Mammoth Russian) plants is the same with either ammonium or nitrate, but nitrate results in an increased proportion of total oxidized plus reduced NADP (NADP[H]) seemingly at the expense of NAD. The activity of NADH- and NADPH-dependent forms of glutamic acid dehydrogenase is correlated with the ratio of total oxidized plus reduced NAD to NADP(H). The low NAD: NADH ratio maintained in nitrate roots despite active NADH utilization via nitrate reductase and glutamic acid dehydrogenase may be the result of nitrate-stimulated glycolysis. Nitrate roots also maintain a high level of NADPH, presumably by the stimulatory effect of nitrate utilization on glucose-6-phosphate dehydrogenase activity. In the presence of nitrate rather than ammonium, the highly active nitrate-reducing leaves of soybean show a greater proportion of total pyridine nucleotide in the form of NADP(H) than do the inactive leaves of sunflower.For all tissues examined, ammonium nutrition yields a higher concentration of total adenine nucleotide than is found with nitrate. The data indicate the production of a higher level of metabolites that enter into purine synthesis with ammonium than with nitrate. Glutamine synthetase activity can be correlated with the concept that enzymes utilizing ATP for biosynthetic purposes increase in activity in accordance with the energy level of the cell.

[1]  T. Kosuge,et al.  Regulation of Enzyme Activity in Photosynthetic Systems , 1970 .

[2]  E. Stadtman,et al.  The regulation of glutamine synthesis in microorganisms. , 1970, Annual review of microbiology.

[3]  L. H. Weinstein,et al.  Acid-soluble nucleotides of pinto bean leaves at different stages of development. , 1969, Plant physiology.

[4]  A. Beevers,et al.  Nitrate Reduction in Higher Plants , 1969 .

[5]  A. Keys,et al.  Root Growth with Ammonium and Nitrate in Continuous Flow Nutrient Solution , 1969 .

[6]  D. E. Atkinson Regulation of enzyme function. , 1969, Annual review of microbiology.

[7]  M. Avron,et al.  A specific and sensitive method for the determination of NADPH. , 1969, Analytical biochemistry.

[8]  R. Leech,et al.  An NADP-dependent L-glutamate dehydrogenase from chloroplasts of Vicia faba L. , 1968, Biochemical and biophysical research communications.

[9]  A. Keys The intracellular distribution of free nucleotides in the tobacco leaf. Formation of adenosine 5'-phosphate from adenosine 5'-triphosphate in the chloroplasts. , 1968, The Biochemical journal.

[10]  M. Avron,et al.  Phosphoadenosine diphosphate ribose, a specific inhibitor of nicotinamide adenine dinucleotide phosphate enzymes. , 1967, The Journal of biological chemistry.

[11]  E. A. Kirkby,et al.  Ionic balance in different tissues of the tomato plant in relation to nitrate, urea, or ammonium nutrition. , 1967, Plant physiology.

[12]  G. S. Weissman Effect of Ammonium and Nitrate Nutrition on Protein Level and Exudate Composition. , 1964, Plant physiology.

[13]  Y. Yamamoto Pyridine Nucleotide Content in the Higher Plant. Effect of Age of Tissue. , 1963, Plant physiology.

[14]  O. H. Lowry,et al.  The stability of pyridine nucleotides. , 1961, The Journal of biological chemistry.

[15]  H. Beevers,et al.  The regulation of pathways of glucose catabolism in maize roots. , 1961, The Biochemical journal.

[16]  G. S. Weissman INFLUENCE OF AMMONIUM AND NITRATE ON THE PROTEIN‐ AND FREE AMINO ACIDS IN SHOOTS OF WHEAT SEEDLINGS , 1959 .

[17]  A. Jagendorf,et al.  A TPNH diaphorase from chloroplasts. , 1956, Archives of biochemistry and biophysics.

[18]  P. Mclean,et al.  The determination of oxidized and reduced diphosphopyridine nucleotide and triphosphopyridine nucleotide in animal tissues. , 1955, The Biochemical journal.

[19]  A. Willis,et al.  THE RESPIRATION OF BARLEY PLANTS. VIII. NITROGEN ASSIMILATION AND THE RESPIRATION OF THE ROOT SYSTEM , 1955 .

[20]  H. Mckee REVIEW OF RECENT WORK ON NITROGEN METABOLISM , 1949 .

[21]  J. Nance THE ROLE OF OXYGEN IN NITRATE ASSIMILATION BY WHEAT ROOTS , 1948 .

[22]  A. R. Ling,et al.  Plant Biochemistry. , 1931, Nature.