Quantification of Virus Particles Using Nanopore-Based Resistive-Pulse Sensing Techniques

Viruses have drawn much attention in recent years due to increased recognition of their important roles in virology, immunology, clinical diagnosis, and therapy. Because the biological and physical properties of viruses significantly impact their applications, quantitative detection of individual virus particles has become a critical issue. However, due to various inherent limitations of conventional enumeration techniques such as infectious titer assays, immunological assays, and electron microscopic observation, this issue remains challenging. Thanks to significant advances in nanotechnology, nanostructure-based electrical sensors have emerged as promising platforms for real-time, sensitive detection of numerous bioanalytes. In this paper, we review recent progress in nanopore-based electrical sensing, with particular emphasis on the application of this technique to the quantification of virus particles. Our aim is to provide insights into this novel nanosensor technology, and highlight its ability to enhance current understanding of a variety of viruses.

[1]  R W DeBlois,et al.  Sizes and concentrations of several type C oncornaviruses and bacteriophage T2 by the resistive-pulse technique , 1977, Journal of virology.

[2]  E. Uzgiris,et al.  Length of glycoprotein spikes of vesicular stomatitis virus and Sindbis virus, measured in situ using quasi elastic light scattering and a resistive-pulse technique. , 1978, Virology.

[3]  E. Uzgiris,et al.  Comparative measurements of size and polydispersity of several insect viruses. , 1978, Analytical biochemistry.

[4]  Paul K. Hansma,et al.  Biological applications of the AFM: From single molecules to organs , 1997 .

[5]  W. Häberle,et al.  AFM review study on pox viruses and living cells. , 1997, Biophysical journal.

[6]  B. L. Smith,et al.  Biological applications of the AFM: From single molecules to organs , 1997, Int. J. Imaging Syst. Technol..

[7]  Kevin Ke,et al.  Submicrometer pore-based characterization and quantification of antibody-virus interactions. , 2006, Small.

[8]  Z. Siwy,et al.  Nanopore analytics: sensing of single molecules. , 2009, Chemical Society reviews.

[9]  Mitchel J Doktycz,et al.  Atomic force microscopy of biological samples. , 2010, Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology.

[10]  Jörg P Kutter,et al.  Nanofluidic devices with two pores in series for resistive-pulse sensing of single virus capsids. , 2011, Analytical chemistry.

[11]  Heide Schatten,et al.  Low voltage high-resolution SEM (LVHRSEM) for biological structural and molecular analysis. , 2011, Micron.

[12]  Geoff R. Willmott,et al.  Quantitative sizing of nano/microparticles with a tunable elastomeric pore sensor. , 2011, Analytical chemistry.

[13]  S. Jacobson,et al.  Characterization of hepatitis B virus capsids by resistive-pulse sensing. , 2011, Journal of the American Chemical Society.

[14]  Matt Trau,et al.  Advances in Resistive Pulse Sensors: Devices bridging the void between molecular and microscopic detection. , 2011, Nano today.

[15]  E. Peterman,et al.  Single Molecule Analysis , 2011, Methods in Molecular Biology.

[16]  Erkki Ruoslahti,et al.  A high-throughput label-free nanoparticle analyser. , 2011, Nature nanotechnology.

[17]  R. Tripp,et al.  One-step assay for detecting influenza virus using dynamic light scattering and gold nanoparticles. , 2011, The Analyst.

[18]  R. Bashir,et al.  Nanopore sensors for nucleic acid analysis. , 2011, Nature nanotechnology.

[19]  Mark W Grinstaff,et al.  Tunable pores for measuring concentrations of synthetic and biological nanoparticle dispersions. , 2012, Biosensors & bioelectronics.

[20]  Mauricio G Mateu,et al.  Mechanical properties of viruses analyzed by atomic force microscopy: a virological perspective. , 2012, Virus research.

[21]  M. Peterka,et al.  Evaluation of nanoparticle tracking analysis for total virus particle determination , 2012, Virology Journal.

[22]  Mark Platt,et al.  Resistive pulse sensing of analyte-induced multicomponent rod aggregation using tunable pores. , 2012, Small.

[23]  M Selim Ünlü,et al.  Single nanoparticle detectors for biological applications. , 2012, Nanoscale.

[24]  M. Trau,et al.  Simultaneous size and ζ-potential measurements of individual nanoparticles in dispersion using size-tunable pore sensors. , 2012, ACS nano.

[25]  J. Betton,et al.  Sensing proteins through nanopores: fundamental to applications. , 2012, ACS chemical biology.

[26]  G. Willmott,et al.  A variable pressure method for characterizing nanoparticle surface charge using pore sensors. , 2012, Analytical chemistry.

[27]  L. Lagae,et al.  Measuring the electric charge and zeta potential of nanometer-sized objects using pyramidal-shaped nanopores. , 2012, Analytical Chemistry.

[28]  Peixuan Guo,et al.  Solid-State and Biological Nanopore for Real-Time Sensing of Single Chemical and Sequencing of DNA. , 2013, Nano today.

[29]  Daniel H Stoloff,et al.  Recent trends in nanopores for biotechnology. , 2013, Current opinion in biotechnology.

[30]  Joshua B Edel,et al.  Single molecule sensing with solid-state nanopores: novel materials, methods, and applications. , 2013, Chemical Society reviews.

[31]  G. Willmott,et al.  Size and charge characterisation of a submicrometre oil-in-water emulsion using resistive pulse sensing with tunable pores. , 2013, Journal of colloid and interface science.

[32]  Jadranka Travas-Sejdic,et al.  Detection of target-probe oligonucleotide hybridization using synthetic nanopore resistive pulse sensing. , 2013, Biosensors & bioelectronics.

[33]  Joshua B. Edel,et al.  Single Molecule Sensing with Solid‐State Nanopores: Novel Materials, Methods, and Applications , 2013 .

[34]  The Role of Pore Geometry in Single Particle Detection , 2013 .

[35]  A Gel Filtration-Based Method for the Purification of Infectious Rotavirus Particles for Environmental Research Applications , 2013, Food and Environmental Virology.

[36]  Matt Trau,et al.  A comparative study of submicron particle sizing platforms: accuracy, precision and resolution analysis of polydisperse particle size distributions. , 2013, Journal of colloid and interface science.

[37]  G. Willmott,et al.  Magnetic microbead transport during resistive pulse sensing. , 2013, Biomicrofluidics.

[38]  S. Kong,et al.  Monitoring bacterial growth using tunable resistive pulse sensing with a pore-based technique , 2013, Applied Microbiology and Biotechnology.

[39]  M. Grinstaff,et al.  Microscopy and tunable resistive pulse sensing characterization of the swelling of pH-responsive, polymeric expansile nanoparticles. , 2013, Nanoscale.

[40]  L. Lagae,et al.  Measuring mass of nanoparticles and viruses in liquids with nanometer-scale pores. , 2014, Analytical chemistry.

[41]  C. Metzner,et al.  Quantitative real-time single particle analysis of virions , 2014, Virology.

[42]  G. Willmott,et al.  MODELLING OF RESISTIVE PULSE SENSING: FLEXIBLE METHODS FOR SUBMICRON PARTICLES , 2014, The ANZIAM Journal.

[43]  G. Willmott,et al.  Nanoparticle ζ-potential measurements using tunable resistive pulse sensing with variable pressure. , 2014, Journal of colloid and interface science.

[44]  Jay X. Tang,et al.  Stiff filamentous virus translocations through solid-state nanopores , 2013, Nature Communications.

[45]  Yi-Lun Ying,et al.  Single molecule analysis by biological nanopore sensors. , 2014, The Analyst.

[46]  Aaron R. Hawkins,et al.  Correlated Electrical and Optical Analysis of Single Nanoparticles and Biomolecules on a Nanopore-Gated Optofluidic Chip , 2014, Nano letters.

[47]  G. Willmott,et al.  Co-ordinated detection of microparticles using tunable resistive pulse sensing and fluorescence spectroscopy. , 2015, Biomicrofluidics.

[48]  J. Harris.  Transmission electron microscopy in molecular structural biology: A historical survey. , 2015, Archives of biochemistry and biophysics.

[49]  S. Jacobson,et al.  Monitoring Assembly of Virus Capsids with Nanofluidic Devices. , 2015, ACS nano.

[50]  Matt Trau,et al.  Analysis of exosome purification methods using a model liposome system and tunable-resistive pulse sensing , 2015, Scientific Reports.

[51]  M. Kirpichnikov,et al.  Comparative Study of Non-Enveloped Icosahedral Viruses Size , 2015, PloS one.

[52]  Matt Trau,et al.  Observations of Tunable Resistive Pulse Sensing for Exosome Analysis: Improving System Sensitivity and Stability. , 2015, Langmuir : the ACS journal of surfaces and colloids.

[53]  S. Jacobson,et al.  Single-Particle Electrophoresis in Nanochannels , 2014, Analytical chemistry.

[54]  Stephen C. Jacobson,et al.  Fundamental Studies of Nanofluidics: Nanopores, Nanochannels, and Nanopipets , 2014, Analytical chemistry.

[55]  Eva Weatherall,et al.  Applications of tunable resistive pulse sensing. , 2015, The Analyst.

[56]  Emma L. C. J. Blundell,et al.  Emergence of tunable resistive pulse sensing as a biosensor , 2015 .

[57]  Stephen C Jacobson,et al.  Conductivity-based detection techniques in nanofluidic devices. , 2015, The Analyst.

[58]  John Yin,et al.  Characterization of vesicular stomatitis virus populations by tunable resistive pulse sensing. , 2015, Journal of virological methods.

[59]  Hongwen Wu,et al.  Translocation of Rigid Rod-Shaped Virus through Various Solid-State Nanopores. , 2016, Analytical chemistry.

[60]  E. Peterman.  Single Molecule Analysis , 2018, Methods in Molecular Biology.