Compositions and Positional Distributions of Fatty Acids in Phospholipids from Leaves of Chilling-Sensitive and Chilling-Resistant Plants :

The compositions and positional distributions of fatty acids in the major leafphospholipids of phosphatidylg lycerol, phosphatidylcholine and phosphatidylethanolamine were analyzed by gas-liquid chromatography and enzymic hydrolysis, and chilling-sensitive and chilling-resistant plants were compared with respect to the relative contents of palmitic and (ranj-jd3-hexadecenoic acids in the separated phospholipids. A distinct difference between these plants was found in the fatty acid compositions of phosphatidylglycerol, in which the sum of palmitic and trans-A 3-hexadecenoic acids ranged from 60 to 78% of the total fatty acids in 8 species of chilling-sensitive plants, and from 50 to 57% in 11 species of chilling-resistant plants. The only exception among the chillingsensitive plants in this respect was the tomato, in which the sum of palmitic and trans^]3-hexadecenoic acids in phosphatidylglycerol amounted to 54%. The fatty acid compositions and the positional distributions of fatty acids in phosphatidylg lycerol suggest that the occurrence of high proportions of dipalmitoyl and l-palmitoyl-2-((raRi-/J3hexadecenoyl) species in this lipid is correlated with the susceptibility to chilling of the leaves of higher plants. In the compositions and positional distributions of fatty acids in phosphatidylcholine and phosphatidylethanolamine, no difference was found between the chilling-sensitive and chilling-resistant plants.

[1]  N. Murata,et al.  Chilling-Susceptibility of the Blue-Green Alga Anacystis nidulans: III. LIPID PHASE OF CYTOPLASMIC MEMBRANE. , 1982, Plant physiology.

[2]  D. C. Fork,et al.  Phase transition temperatures determined for chloroplast thylakoid membranes and for liposomes prepared from charged lipids extracted from thylakoid membranes of higher plants , 1981 .

[3]  N. Murata,et al.  Chilling Susceptibility of the Blue-green Alga Anacystis nidulans: II. STIMULATION OF THE PASSIVE PERMEABILITY OF CYTOPLASMIC MEMBRANE AT CHILLING TEMPERATURES. , 1981, Plant physiology.

[4]  N. Murata,et al.  Chilling Susceptibility of the Blue-green Alga Anacystis nidulans: I. EFFECT OF GROWTH TEMPERATURE. , 1981, Plant physiology.

[5]  J. Berry,et al.  Membrane phospholipid phase separations in plants adapted to or acclimated to different thermal regimes. , 1980, Plant physiology.

[6]  J. Harwood.  Plant Acyl Lipids: Structure, Distribution, and Analysis , 1980 .

[7]  K. Keough,et al.  Gel to liquid-crystalline phase transitions in water dispersions of saturated mixed-acid phosphatidylcholines. , 1979, Biochemistry.

[8]  M. Kito,et al.  Changes in the phospholipid molecular species composition of soybean hypocotyl and cotyledon after dedifferentiation. , 1978, Biochimica et biophysica acta.

[9]  D. C. Fork,et al.  Temperature dependence of the light-induced spectral shift of carotenoids in Cyanidium caldarium and higher plant leaves. Evidence for an effect of the physical phase of chloroplast membrane lipids on the permeability of the membrane to ions. , 1977, Biochimica et biophysica acta.

[10]  F. Gunstone,et al.  Hydrocarbon chain packing and molecular motion in phospholipid bilayers formed from unsaturated lecithins. Synthesis and properties of sixteen positional isomers of 1,2-dioctadecenoyl-sn-glycero-3-phosphorylcholine. , 1975, The Journal of biological chemistry.

[11]  K. Jacobson,et al.  Phase transitions and phase separations in phospholipid membranes induced by changes in temperature, pH, and concentration of bivalent cations. , 1975, Biochemistry.

[12]  A. Benson,et al.  Isolation and properties of the envelope of spinach chloroplasts. , 1973, The Journal of biological chemistry.

[13]  H. Mcconnell,et al.  Lateral phase separation in phospholipid membranes. , 1973, Biochemistry.

[14]  J. Lyons.  CHILLING INJURY IN PLANTS , 1973 .

[15]  B. de Kruyff,et al.  The effect of the polar headgroup on the lipid-cholesterol interaction: a monolayer and differential scanning calorimetry study. , 1973, Biochimica et biophysica acta.

[16]  J. K. Raison.  The influence of temperature-induced phase changes on the kinetics of respiratory and other membrane-associated enzyme systems. , 1973, Journal of bioenergetics.

[17]  E. Heinz,et al.  The suitability of lipase from Rhizopus arrhizus delemar for analysis of fatty acid distribution in dihexosyl diglycerides, phospholipids and plant sulfolipids. , 1973, Hoppe-Seyler's Zeitschrift fur physiologische Chemie.

[18]  J. Sturtevant,et al.  Calorimetric studies of dilute aqueous suspensions of bilayers formed from synthetic L- -lecithins. , 1972, The Journal of biological chemistry.

[19]  H. Hauser,et al.  The inter- and intra-molecular mixing of hydrocarbon chains in lecithin-water systems. , 1972, Chemistry and physics of lipids.

[20]  F. Haverkate,et al.  Isolation and chemical characterization of phosphatidyl glycerol from spinach leaves. , 1965, Biochimica et biophysica acta.

[21]  F. Shorland,et al.  THE ISOLATION OF TRANS-3-HEXADECENOIC ACID FROM THE LIPIDS OF RED-CLOVER (TRIFOLIUM PRATENSE) LEAVES. , 1964, Biochimica et biophysica acta.

[22]  J. Wintermans.  Concentrations of phosphatides and glycolipids in leaves and chloroplasts. , 1960, Biochimica et biophysica acta.

[23]  W. J. Dyer,et al.  A rapid method of total lipid extraction and purification. , 1959, Canadian journal of biochemistry and physiology.