High scleroglucan production by Sclerotium rolfsii: Influence of medium composition

Scleroglucan production by Sclerotium rolfsii was markedly affected by the C-source concentration, showing a highest value with 150 g sucrose l−1. Production was also influenced by the N-source, being considerably higher in media containing NO3− than in those containing NH4, which had a clear inhibitory effect. Once defined the optimum culture medium composition, the highest exopolysaccharide production (ca. 26 g scleroglucan l−1) was achieved after 72 h of fermentation at shake flask scale. High values of yield (Yp/c = 0.49), productivity (Pr = 0.365 g l−1 h−1) and specific productivity (Pr/x = 0.031 g (g biomass)−1 h−1) were observed, and productivity was 1.5 times further increased by scalling-up to fermenter scale. Addition of L-threonine, sunflower oil and ascorbic acid diminished exopolysaccharide production. © Rapid Science Ltd. 1998

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