Regulation of Chromatin Remodeling by Inositol Polyphosphates

Chromatin remodeling is required for efficient transcription of eukaryotic genes. In a genetic selection for budding yeast mutants that were defective in induction of the phosphate-responsive PHO5 gene, we identified mutations inARG82/IPK2, which encodes a nuclear inositol polyphosphate kinase. In arg82 mutant strains, remodeling ofPHO5 promoter chromatin is impaired, and the adenosine triphosphate–dependent chromatin-remodeling complexes SWI/SNF and INO80 are not efficiently recruited to phosphate-responsive promoters. These results suggest a role for the small molecule inositol polyphosphate in the regulation of chromatin remodeling and transcription.

[1]  V. Iyer,et al.  The chromo domain protein Chd1p from budding yeast is an ATP‐dependent chromatin‐modifying factor , 2000, The EMBO journal.

[2]  E. O’Shea,et al.  Phosphorylation regulates association of the transcription factor Pho4 with its import receptor Pse1/Kap121. , 1998, Genes & development.

[3]  J. Schmitz,et al.  The transactivation domain of Pho4 is required for nucleosome disruption at the PHO5 promoter. , 1994, The EMBO journal.

[4]  B. Cairns,et al.  Activation domain-mediated targeting of the SWI/SNF complex to promoters stimulates transcription from nucleosome arrays. , 1999, Molecular cell.

[5]  S. Snyder,et al.  The Inositol Hexakisphosphate Kinase Family , 2000, Journal of Biological Chemistry.

[6]  J. Palmer,et al.  Characterization of the imitation switch subfamily of ATP-dependent chromatin-remodeling factors in Saccharomyces cerevisiae. , 1999, Genes & development.

[7]  E. Dubois,et al.  Inositol polyphosphate kinase activity of Arg82/ArgRIII is not required for the regulation of the arginine metabolism in yeast , 2000, FEBS letters.

[8]  E. O’Shea,et al.  Roles of phosphorylation sites in regulating activity of the transcription factor Pho4. , 1999, Science.

[9]  M. Scott,et al.  Five SWI/SNF gene products are components of a large multisubunit complex required for transcriptional enhancement. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[10]  Michael Grunstein,et al.  Global histone acetylation and deacetylation in yeast , 2000, Nature.

[11]  S. Snyder,et al.  Inositol polyphosphate multikinase (ArgRIII) determines nuclear mRNA export in Saccharomyces cerevisiae , 2000, FEBS letters.

[12]  R. Kingston,et al.  Cooperation between Complexes that Regulate Chromatin Structure and Transcription , 2002, Cell.

[13]  Ali Hamiche,et al.  A chromatin remodelling complex involved in transcription and DNA processing , 2000, Nature.

[14]  A. Hinnen,et al.  Removal of positioned nucleosomes from the yeast PHO5 promoter upon PHO5 induction releases additional upstream activating DNA elements. , 1986, The EMBO journal.

[15]  A. Schmid,et al.  Increasing the rate of chromatin remodeling and gene activation—a novel role for the histone acetyltransferase Gcn5 , 2001, The EMBO journal.

[16]  J. York,et al.  A phospholipase C-dependent inositol polyphosphate kinase pathway required for efficient messenger RNA export. , 1999, Science.

[17]  J. York,et al.  A role for nuclear inositol 1,4,5-trisphosphate kinase in transcriptional control. , 2000, Science.

[18]  C Logie,et al.  Recruitment of the SWI/SNF chromatin remodeling complex by transcriptional activators. , 1999, Genes & development.

[19]  E. O’Shea,et al.  Phosphorylation of the transcription factor PHO4 by a cyclin-CDK complex, PHO80-PHO85. , 1994, Science.

[20]  Wei-Hua Wu,et al.  Modulation of ATP-Dependent Chromatin-Remodeling Complexes by Inositol Polyphosphates , 2002, Science.

[21]  C. Allis,et al.  Translating the Histone Code , 2001, Science.

[22]  Y. Oshima The phosphatase system in Saccharomyces cerevisiae. , 1997, Genes & genetic systems.

[23]  J. Schmitz,et al.  Role of trans‐activating proteins in the generation of active chromatin at the PHO5 promoter in S. cerevisiae. , 1990, The EMBO journal.

[24]  E. Dubois,et al.  Recruitment of the yeast MADS‐box proteins, ArgRI and Mcm1 by the pleiotropic factor ArgRIII is required for their stability , 2000, Molecular microbiology.