Antibodies against human papillomavirus type 16 and 18 E6 and E7 proteins in cervicovaginal washings and serum of patients with cervical neoplasia.

Serum antibodies against the E6 and E7 proteins of human papillomavirus (HPV) 16 and 18 are associated with cervical cancer. The aim of this study was to investigate the presence of local antibodies against HPV in cervicovaginal washings (CWs). In this study antibodies against the native HPV16 and HPV18 E6/E7 proteins were detectable in CWs (48%) and sera (29%) from patients with cervical cancer (n = 21) utilizing a sandwich protein enzyme-linked immunosorbent assay (ELISA). In paired CWs and sera from patients with cervical intraepithelial neoplasia (n = 38) and from healthy women (n = 22) no antibodies against these proteins were found. In 10 of 11 patients, the antibody response corresponded with the HPV type in the cervical smear and/or tumor tissue, which indicates the HPV type specificity of the assay. In 7 of 11 patients with antibody reactivity against HPV16 or HPV18 E6 and/or E7 proteins a higher level of antibody reactivity in the CWs than in the paired serum samples was found at similar inputs of total IgG. This suggests that the antibodies in the CWs against the investigated HPV proteins in these patients were locally produced.

[1]  T. Out,et al.  IgG antibodies against human papillomavirus type 16 E7 proteins in cervicovaginal washing fluid from patients with cervical neoplasia. , 2000, International journal of gynecological cancer : official journal of the International Gynecological Cancer Society.

[2]  M. Pawlita,et al.  Absence of antibody against human papillomavirus type 16 E6 and E7 in patients with cervical cancer is independent of sequence variations. , 2000, The Journal of infectious diseases.

[3]  N. Kiviat,et al.  Detection of cervical antibodies to human papillomavirus type 16 (HPV-16) capsid antigens in relation to detection of HPV-16 DNA and cervical lesions. , 2000, The Journal of infectious diseases.

[4]  M. Pawlita,et al.  Antibodies against oncoproteins E6 and E7 of human papillomavirus types 16 and 18 in cervical‐carcinoma patients from Russia , 2000, International journal of cancer.

[5]  T. Out,et al.  Increased IL-6 and IL-8 levels in cervicovaginal secretions of patients with cervical cancer. , 1999, Gynecologic oncology.

[6]  J. Dillner,et al.  Immune responses against human papillomavirus (HPV) type 16 virus-like particles in a cohort study of women with cervical intraepithelial neoplasia. II. Systemic but not local IgA responses correlate with clearance of HPV-16. , 1999, The Journal of general virology.

[7]  H. Hollema,et al.  Telomerase activity as a biomarker for (pre)neoplastic cervical disease in scrapings and frozen sections from patients with abnormal cervical smear. , 1998, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.

[8]  S. J. Kim,et al.  HPV-16-related proteins as the serologic markers in cervical neoplasia. , 1998, Gynecologic oncology.

[9]  C. H. Buckley,et al.  Aetiology, pathogenesis, and pathology of cervical neoplasia. , 1998, Journal of clinical pathology.

[10]  M. Pawlita,et al.  Antibodies against Early Proteins of Human Papillomaviruses as Diagnostic Markers for Invasive Cervical Cancer , 1998, Journal of Clinical Microbiology.

[11]  H. Hollema,et al.  Relation between HPV-16 serology and clinico-pathological data in cervical carcinoma patients: prognostic value of anti-E6 and/or anti-E7 antibodies , 1997, Cancer Immunology, Immunotherapy.

[12]  Harald zur Hausen,et al.  Papillomavirus infections — a major cause of human cancers , 1996 .

[13]  L. Gissmann.  Immunologic responses to human papillomavirus infection. , 1996, Obstetrics and gynecology clinics of North America.

[14]  E. Stockfleth,et al.  The association of human papillomavirus type 16 E6 and E7 antibodies with stage of cervical cancer. , 1996, Gynecologic oncology.

[15]  H. Gore,et al.  Normal Uterine Cervix: Characterization of Isolated Lymphocyte Phenotypes and Immunoglobulin Secretion , 1995, American journal of reproductive immunology.

[16]  H. Hocini,et al.  Systemic and Secretory Humoral Immunity in the Normal Human Vaginal Tract , 1995, Scandinavian journal of immunology.

[17]  K. Nooter,et al.  Absence of human papillomavirus DNA from esophageal carcinoma as determined by multiple broad spectrum polymerase chain reactions , 1995, Journal of medical virology.

[18]  V. Moreno,et al.  Prevalence of Human Papillomavirus in Cervical Cancer: a Worldwide Perspective , 1995 .

[19]  S. J. Kim,et al.  Immunologic diagnosis and monitoring of cervical cancers using in vitro translated HPV proteins. , 1995, Gynecologic oncology.

[20]  M. Dreyfus,et al.  Seric and local antibodies against a synthetic peptide of HPV16. , 1995, European journal of obstetrics, gynecology, and reproductive biology.

[21]  J. Kónya,et al.  Human papillomavirus DNA and anti‐HPV secretory IgA antibodies in cytologically normal cervical specimens , 1994, Journal of medical virology.

[22]  R. Scully,et al.  Histological Typing of Female Genital Tract Tumours , 1994, World Health Organization International Histological Classification of Tumours.

[23]  J. Bouwes Bavinck,et al.  Detection of cutaneous and genital HPV types in clinical samples by PCR using consensus primers. , 1993, Journal of virological methods.

[24]  J. Dillner,et al.  Antibodies against papillomavirus antigens in cervical secretions from condyloma patients , 1993, Journal of clinical microbiology.

[25]  F. X. Bosch,et al.  Antibodies to HPV-16 E6 and E7 proteins as markers for HPV-16-associated invasive cervical cancer. , 1992, Virology.

[26]  Galloway Da.  Serological assays for the detection of HPV antibodies. , 1992 .

[27]  J. Blomberg,et al.  Detection of iga antibodies against human papillomavirus in cervical secretions from patients with cervical intraepithelial neoplasia , 1989, International journal of cancer.

[28]  G. Temple,et al.  Oncogenic association of specific human papillomavirus types with cervical neoplasia. , 1987, Journal of the National Cancer Institute.

[29]  T. Out,et al.  Immunoglobulin production in human mixed lymphocyte cultures: implications for co-cultures of cells from patients and healthy donors. , 1982, Journal of immunology.

[30]  R. Weller,et al.  International Histological Classification of Tumours , 1981 .

[31]  E. Wachtel.  International Histological Classification of Tumours No. 8 Cytology of the Female Genital Tract , 1974 .