Cancer Cell Membrane-Biomimetic Nanoparticles for Homologous-Targeting Dual-Modal Imaging and Photothermal Therapy.

An active cell membrane-camouflaged nanoparticle, owning to membrane antigens and membrane structure, can achieve special properties such as specific recognition, long blood circulation, and immune escaping. Herein, we reported a cancer cell membrane-cloaked nanoparticle system as a theranostic nanoplatform. The biomimetic nanoparticles (indocyanine green (ICG)-loaded and cancer cell membrane-coated nanoparticles, ICNPs) exhibit a core-shell nanostructure consisting of an ICG-polymeric core and cancer cell membrane shell. ICNPs demonstrated specific homologous targeting to cancer cells with good monodispersity, preferable photothermal response, and excellent fluorescence/photoacoustic (FL/PA) imaging properties. Benefited from the functionalization of the homologous binding adhesion molecules from cancer cell membranes, ICNPs significantly promoted cell endocytosis and homologous-targeting tumor accumulation in vivo. Moreover, ICNPs were also good at disguising as cells to decrease interception by the liver and kidney. Through near-infrared (NIR)-FL/PA dual-modal imaging, ICNPs could realize real-time monitored in vivo dynamic distribution with high spatial resolution and deep penetration. Under NIR laser irradiation, ICNPs exhibited highly efficient photothermal therapy to eradicate xenografted tumor. The robust ICNPs with homologous properties of cancer cell membranes can serve as a bionic nanoplatform for cancer-targeted imaging and phototherapy.

[1]  Ronnie H. Fang,et al.  A biomimetic nanosponge that absorbs pore-forming toxins , 2013, Nature nanotechnology.

[2]  Y. Gun’ko,et al.  Preparation of multifunctional nanoparticles and their assemblies , 2012, Nature Protocols.

[3]  K. Pienta,et al.  Intravascular metastatic cancer cell homotypic aggregation at the sites of primary attachment to the endothelium. , 2003, Cancer research.

[4]  Ronnie H. Fang,et al.  Nanoparticle-detained toxins for safe and effective vaccination , 2013, Nature nanotechnology.

[5]  Michael Riediker,et al.  Therapeutic nanoparticles in clinics and under clinical evaluation. , 2013, Nanomedicine.

[6]  O. Farokhzad.  Nanotechnology: Platelet mimicry , 2015, Nature.

[7]  Fei Wang,et al.  Detoxification of Organophosphate Poisoning Using Nanoparticle Bioscavengers. , 2015, ACS nano.

[8]  Yoshihiro Ito,et al.  A fusion protein N-cadherin-Fc as an artificial extracellular matrix surface for maintenance of stem cell features. , 2010, Biomaterials.

[9]  Naomi J Halas,et al.  Theranostic nanoshells: from probe design to imaging and treatment of cancer. , 2011, Accounts of chemical research.

[10]  W. Liu,et al.  Cancer Cell Membrane‐Coated Upconversion Nanoprobes for Highly Specific Tumor Imaging , 2016, Advanced materials.

[11]  G. Rabinovich,et al.  Immunosuppressive strategies that are mediated by tumor cells. , 2007, Annual review of immunology.

[12]  Ronnie H. Fang,et al.  Cancer Cell Membrane-Coated Nanoparticles for Anticancer Vaccination and Drug Delivery , 2014, Nano letters.

[13]  Raimo Hartmann,et al.  Surface Functionalization of Nanoparticles with Polyethylene Glycol: Effects on Protein Adsorption and Cellular Uptake. , 2015, ACS nano.

[14]  Weiping Zou,et al.  Immunosuppressive networks in the tumour environment and their therapeutic relevance , 2005, Nature Reviews Cancer.

[15]  Zhiguang Wu,et al.  Cell‐Membrane‐Coated Synthetic Nanomotors for Effective Biodetoxification , 2015 .

[16]  Stuart A. Aaronson,et al.  Exogenous Expression of N-Cadherin in Breast Cancer Cells Induces Cell Migration, Invasion, and Metastasis , 2000, The Journal of cell biology.

[17]  Dezhi Ni,et al.  Erythrocyte membrane-coated NIR-triggered biomimetic nanovectors with programmed delivery for photodynamic therapy of cancer. , 2015, Nanoscale.

[18]  Ronnie H. Fang,et al.  Erythrocyte membrane-camouflaged polymeric nanoparticles as a biomimetic delivery platform , 2011, Proceedings of the National Academy of Sciences.

[19]  Junbai Li,et al.  Macrophage Cell Membrane Camouflaged Au Nanoshells for in Vivo Prolonged Circulation Life and Enhanced Cancer Photothermal Therapy. , 2016, ACS applied materials & interfaces.

[20]  Ping Gong,et al.  Smart human serum albumin-indocyanine green nanoparticles generated by programmed assembly for dual-modal imaging-guided cancer synergistic phototherapy. , 2014, ACS nano.

[21]  Zhen Gu,et al.  Anticancer Platelet‐Mimicking Nanovehicles , 2015, Advanced materials.

[22]  Yifan Ma,et al.  Single-step assembly of DOX/ICG loaded lipid--polymer nanoparticles for highly effective chemo-photothermal combination therapy. , 2013, ACS nano.

[23]  Anne L. van de Ven,et al.  Synthetic nanoparticles functionalized with biomimetic leukocyte membranes possess cell-like functions. , 2013, Nature nanotechnology.

[24]  Lintao Cai,et al.  Improving drug accumulation and photothermal efficacy in tumor depending on size of ICG loaded lipid-polymer nanoparticles. , 2014, Biomaterials.

[25]  G. Glinsky,et al.  MDA-MB-435 Human Breast Carcinoma Cell Homo- and Heterotypic Adhesion under Flow Conditions Is Mediated in Part by Thomsen-Friedenreich Antigen-Galectin-3 Interactions* , 2003, The Journal of Biological Chemistry.

[26]  Dennis E Discher,et al.  Minimal " Self " Peptides That Inhibit Phagocytic Clearance and Enhance Delivery of Nanoparticles References and Notes , 2022 .

[27]  Erkki Ruoslahti,et al.  Targeting of drugs and nanoparticles to tumors , 2010, The Journal of cell biology.

[28]  Bahman Anvari,et al.  Biodistribution of encapsulated indocyanine green in healthy mice. , 2009, Molecular pharmaceutics.

[29]  Anil K Patri,et al.  Protein corona composition does not accurately predict hematocompatibility of colloidal gold nanoparticles. , 2014, Nanomedicine : nanotechnology, biology, and medicine.

[30]  Vishal Saxena,et al.  Enhanced photo-stability, thermal-stability and aqueous-stability of indocyanine green in polymeric nanoparticulate systems. , 2004, Journal of photochemistry and photobiology. B, Biology.

[31]  D. Xing,et al.  Enhanced tumor treatment using biofunctional indocyanine green-containing nanostructure by intratumoral or intravenous injection. , 2012, Molecular pharmaceutics.

[32]  N. Tinari,et al.  Synthetic inhibitors of galectin-1 and -3 selectively modulate homotypic cell aggregation and tumor cell apoptosis. , 2009, Anticancer research.

[33]  Vishal Saxena,et al.  Polymeric nanoparticulate delivery system for Indocyanine green: biodistribution in healthy mice. , 2006, International journal of pharmaceutics.

[34]  U. Schubert,et al.  Poly(ethylene glycol) in drug delivery: pros and cons as well as potential alternatives. , 2010, Angewandte Chemie.

[35]  Joseph M. DeSimone,et al.  Strategies in the design of nanoparticles for therapeutic applications , 2010, Nature Reviews Drug Discovery.

[36]  Y. Hannun,et al.  EpCAM Is Overexpressed in Breast Cancer and Is a Potential Target for Breast Cancer Gene Therapy , 2004, Cancer Research.

[37]  T. Veenstra,et al.  Identification of membrane proteins from mammalian cell/tissue using methanol-facilitated solubilization and tryptic digestion coupled with 2D-LC-MS/MS , 2007, Nature Protocols.

[38]  H. Dai,et al.  PEG branched polymer for functionalization of nanomaterials with ultralong blood circulation. , 2009, Journal of the American Chemical Society.