Antibody‐mediated glycophorin C coligation on K562 cells induces phosphatidylserine exposure and cell death in an atypical apoptotic process

Glycophorin C (GPC) is necessary in the maintenance of red blood cell structure. Severe autoimmune hemolytic anemia and hemolytic disease of the fetus and newborn (HDFN) have been associated with Gerbich (Ge) blood group system antigens expressed on GPC. Previous in vitro studies with cord blood progenitor cells have shown that anti‐Ge suppresses erythropoiesis.

[1]  A. Wickrema,et al.  Vesicle trafficking plays a novel role in erythroblast enucleation. , 2010, Blood.

[2]  P. Vandenabeele,et al.  Molecular mechanisms of necroptosis: an ordered cellular explosion , 2010, Nature Reviews Molecular Cell Biology.

[3]  G. Denomme,et al.  Anti‐glycophorin C induces mitochondrial membrane depolarization and a loss of extracellular regulated kinase 1/2 protein kinase activity that is prevented by pretreatment with cytochalasin D: implications for hemolytic disease of the fetus and newborn caused by anti‐Ge3 , 2010, Transfusion.

[4]  M. Czyz,et al.  A non‐apoptotic function of caspase‐3 in pharmacologically‐induced differentiation of K562 cells , 2009, British journal of pharmacology.

[5]  N. Mohandas,et al.  Targeted deletion of alpha-adducin results in absent beta- and gamma-adducin, compensated hemolytic anemia, and lethal hydrocephalus in mice. , 2007, Blood.

[6]  Jia Yu,et al.  Differential expression changes in K562 cells during the hemin-induced erythroid differentiation and the phorbol myristate acetate (PMA)-induced megakaryocytic differentiation , 2006, Molecular and Cellular Biochemistry.

[7]  G. Denomme,et al.  Inhibition of erythroid progenitor cell growth by anti‐Ge3 , 2006, British journal of haematology.

[8]  R A Knight,et al.  Classification of cell death: recommendations of the Nomenclature Committee on Cell Death 2009 , 2005, Cell Death and Differentiation.

[9]  N. Avent,et al.  Ligation of CD47 mediates phosphatidylserine expression on erythrocytes and a concomitant loss of viability in vitro , 2005, British journal of haematology.

[10]  N. Avent,et al.  Expression of phosphatidylserine (PS) on wild‐type and Gerbich variant erythrocytes following glycophorin‐C (GPC) ligation , 2005, British journal of haematology.

[11]  S. Glenn,et al.  Late onset neonatal anaemia due to maternal anti‐Ge: possible association with destruction of eythroid progenitors , 2005, Transfusion medicine.

[12]  U. Testa,et al.  Apoptotic mechanisms in the control of erythropoiesis , 2004, Leukemia.

[13]  P. Ferri,et al.  In vitro apoptotic cell death during erythroid differentiation , 2004, Apoptosis.

[14]  P. Gane,et al.  Flow cytometric analysis of the association between blood group‐related proteins and the detergent‐insoluble material of K562 cells and erythroid precursors , 2001, British journal of haematology.

[15]  N. Mivechi,et al.  Role of ERK activation in growth and erythroid differentiation of K562 cells. , 2001, Experimental cell research.

[16]  P. Gane,et al.  Time‐course expression of polypeptides carrying blood group antigens during human erythroid differentiation , 1999, British journal of haematology.

[17]  I. Roberts,et al.  Inhibition of erythroid progenitor cells by anti-Kell antibodies in fetal alloimmune anemia. , 1998, The New England journal of medicine.

[18]  G. Garratty,et al.  Warm autoimmune hemolytic anemia associated with an IgM autoanti-Ge , 1998, Immunohematology.

[19]  A. Schechter,et al.  Hemin-induced acceleration of hemoglobin production in immature cultured erythroid cells: preferential enhancement of fetal hemoglobin. , 1995, Blood.

[20]  A. Chishti,et al.  Evidence that red blood cell protein p55 may participate in the skeleton-membrane linkage that involves protein 4.1 and glycophorin C. , 1993, Blood.

[21]  I. Shulman,et al.  Autoanti‐Ge Associated with Severe Autoimmune Hemolytic Anemia , 1990, Vox sanguinis.

[22]  F. Galibert,et al.  Isolation of cDNA clones and complete amino acid sequence of human erythrocyte glycophorin C. , 1986, The Journal of biological chemistry.

[23]  P. Morel,et al.  Autoimmune Hemolytic Anemia Associated with Autoanti‐Ge , 1981, Vox sanguinis.

[24]  A. Schechter,et al.  Induction of hemoglobin accumulation in human K562 cells by hemin is reversible. , 1981, Science.

[25]  F. Lang,et al.  Ceramide in Suicidal Death of Erythrocytes , 2010, Cellular Physiology and Biochemistry.

[26]  R A Knight,et al.  Classification of cell death: recommendations of the Nomenclature Committee on Cell Death , 2005, Cell Death and Differentiation.