Transduction of chromosomal genes between enteric bacteria by bacteriophage P1

We have used P1 transduction to create intergeneric hybrid strains of enteric bacteria by moving the genA and hut genes between Klebsiella aerogenes, Escherichia coli and Salmonella typhimurium. The use of E. coli as the recipient in such transductions permits the construction of episomes and specialized transducing phage containing non-E. coli material. The effect of host restriction modification and deoxyribonucleic acid homology on the frequency of intergeneric transduction of these loci has been examined.

[1]  B. Magasanik,et al.  Gene order of the histidine utilization (hut) operons in Klebsiella aerogenes , 1975, Journal of bacteriology.

[2]  B. Magasanik,et al.  Genetic control of glutamine synthetase in Klebiella aerogenes , 1975, Journal of bacteriology.

[3]  F. Ausubel,et al.  Regulation of Nitrogen Fixation in Klebsiella pneumoniae: Evidence for a Role of Glutamine Synthetase as a Regulator of Nitrogenase Synthesis , 1974 .

[4]  R. Bender,et al.  Direct Selection for P1-Sensitive Mutants of Enteric Bacteria , 1974, Journal of bacteriology.

[5]  K. Shanmugam,et al.  Deletion mutants of nitrogen fixation in Klebsiella pneumoniae: Mapping of a cluster of nif genes essential for nitrogenase activity , 1974 .

[6]  B. Magasanik,et al.  Glutamine synthetase as a regulator of enzyme synthesis. , 1974, Current topics in cellular regulation.

[7]  B. Magasanik,et al.  Activation of transcription of hut DNA by glutamine synthetase. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[8]  E. Stadtman,et al.  Comparative Biochemical and Immunological Studies of Bacterial Glutamine Synthetases , 1973, Journal of bacteriology.

[9]  R. B. Middleton,et al.  Salmonella typhimurium-Escherichia coli hybrids for the tryptophan region. , 1972, Genetics.

[10]  R. Dixon,et al.  Genetic Transfer of Nitrogen Fixation from Klebsiella pneumoniae to Escherichia coli , 1972, Nature.

[11]  M. Voll.  Derivation of an F-Merogenote and a φ80 High-Frequency Transducing Phage Carrying the Histidine Operon of Salmonella , 1972, Journal of bacteriology.

[12]  R. B. Middleton.  The genetic homology of Salmonella typhimurium and Escherichia coli. , 1971, Genetics.

[13]  B. Magasanik,et al.  Resistance to catabolite repression of histidase and proline oxidase during nitrogen-limited growth of Klebsiella aerogenes. , 1971, The Journal of biological chemistry.

[14]  Gerald R. Smith.  Specialized transduction of the Salmonella hut operons by coliphageλ: Deletion analysis of the hut operons employing λphut , 1971 .

[15]  B. Magasanik,et al.  The two operons of the histidine utilization system in Salmonella typhimurium. , 1971, The Journal of biological chemistry.

[16]  W. Wood,et al.  Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA. , 1966, Journal of molecular biology.