Engineering 3D micro-compartments for highly efficient and scale-independent expansion of human pluripotent stem cells in bioreactors.

[1]  C. Verfaillie,et al.  Scalable expansion of iPSC and their derivatives across multiple lineages. , 2022, Reproductive toxicology.

[2]  M. Vemuri,et al.  A Dynamic 3D Aggregate-Based System for the Successful Expansion and Neural Induction of Human Pluripotent Stem Cells , 2022, Frontiers in Cellular Neuroscience.

[3]  Breanna S Borys,et al.  Cell Culture Process Scale-Up Challenges for Commercial-Scale Manufacturing of Allogeneic Pluripotent Stem Cell Products , 2022, Bioengineering.

[4]  M. S. Kallos,et al.  Induced pluripotency in the context of stem cell expansion bioprocess development, optimization, and manufacturing: a roadmap to the clinic , 2021, NPJ Regenerative medicine.

[5]  Pouria Fattahi,et al.  Core–shell hydrogel microcapsules enable formation of human pluripotent stem cell spheroids and their cultivation in a stirred bioreactor , 2021, Scientific Reports.

[6]  S. Kalies,et al.  High density bioprocessing of human pluripotent stem cells by metabolic control and in silico modeling , 2021, Stem cells translational medicine.

[7]  S. Kaur,et al.  Non-matrigel scaffolds for organoid cultures. , 2021, Cancer letters.

[8]  Breanna S Borys,et al.  Overcoming bioprocess bottlenecks in the large-scale expansion of high-quality hiPSC aggregates in vertical-wheel stirred suspension bioreactors , 2020, Stem cell research & therapy.

[9]  A. Lavoie,et al.  Process development and scale-up of pluripotent stem cell manufacturing , 2020, Cell and Gene Therapy Insights.

[10]  S. Yamanaka.  Pluripotent Stem Cell-Based Cell Therapy-Promise and Challenges. , 2020, Cell stem cell.

[11]  E. Quelennec,et al.  Generation of two induced pluripotent stem cell lines IMAGINi004-A and IMAGINi005-A from healthy donors. , 2020, Stem cell research.

[12]  D. Gao,et al.  Amnion-on-a-chip: modeling human amniotic development in mid-gestation from pluripotent stem cells. , 2020, Lab on a chip.

[13]  Jianping Fu,et al.  Bioengineered pluripotent stem cell models: new approaches to explore early human embryo development. , 2020, Current opinion in biotechnology.

[14]  M. Zernicka-Goetz,et al.  Autophagy-mediated apoptosis eliminates aneuploid cells in a mouse model of chromosome mosaicism , 2020, Nature Communications.

[15]  W. Murphy,et al.  Synthetic alternatives to Matrigel , 2020, Nature Reviews Materials.

[16]  Brian Lee,et al.  Optimized serial expansion of human induced pluripotent stem cells using low‐density inoculation to generate clinically relevant quantities in vertical‐wheel bioreactors , 2020, Stem cells translational medicine.

[17]  Seongkyu Yoon,et al.  Bioprocess Technologies that Preserve the Quality of iPSCs. , 2020, Trends in biotechnology.

[18]  S. Nik-Zainal,et al.  Low rates of mutation in clinical grade human pluripotent stem cells under different culture conditions , 2020, Nature Communications.

[19]  Mary C. Regier,et al.  A Rainbow Reporter Tracks Single Cells and Reveals Heterogeneous Cellular Dynamics among Pluripotent Stem Cells and Their Differentiated Derivatives , 2020, bioRxiv.

[20]  E. Cuppen,et al.  The mutational impact of culturing human pluripotent and adult stem cells , 2018, Nature Communications.

[21]  P. Andrews,et al.  Nucleosides Rescue Replication-Mediated Genome Instability of Human Pluripotent Stem Cells , 2019, bioRxiv.

[22]  Brian Lee,et al.  Strategies for the expansion of human induced pluripotent stem cells as aggregates in single-use Vertical-Wheel™ bioreactors , 2019, Journal of Biological Engineering.

[23]  Y. Shao,et al.  Controlled modelling of human epiblast and amnion development using stem cells , 2019, Nature.

[24]  Aleksandra A. Kolodziejczyk,et al.  The cell cycle in stem cell proliferation, pluripotency and differentiation , 2019, Nature Cell Biology.

[25]  C. Heisenberg,et al.  Mechanochemical Feedback Loops in Development and Disease , 2019, Cell.

[26]  H. Sasaki,et al.  Epiblast formation by Tead-Yap-dependent expression of pluripotency factors and competitive elimination of unspecified cells , 2018, bioRxiv.

[27]  J. Rosenblatt,et al.  The forces and fates of extruding cells. , 2018, Current opinion in cell biology.

[28]  Angelika Amon,et al.  Chromosome Segregation Fidelity in Epithelia Requires Tissue Architecture , 2018, Cell.

[29]  Magdalena Zernicka-Goetz,et al.  Deconstructing and reconstructing the mouse and human early embryo , 2018, Nature Cell Biology.

[30]  Peter W. Zandstra,et al.  Modulating cell state to enhance suspension expansion of human pluripotent stem cells , 2018, Proceedings of the National Academy of Sciences.

[31]  Said Assou,et al.  Concise Review: Assessing the Genome Integrity of Human Induced Pluripotent Stem Cells: What Quality Control Metrics? , 2018, Stem cells.

[32]  Nico Stuurman,et al.  Cellular aspect ratio and cell division mechanics underlie the patterning of cell progeny in diverse mammalian epithelia , 2018, eLife.

[33]  Ou Wang,et al.  Scalable and physiologically relevant microenvironments for human pluripotent stem cell expansion and differentiation , 2018, Biofabrication.

[34]  John C. Marioni,et al.  Pluripotent state transitions coordinate morphogenesis in mouse and human embryos , 2017, Nature.

[35]  Diana Nada Caterina Massai,et al.  Progress and challenges in large-scale expansion of human pluripotent stem cells , 2017 .

[36]  A. Pacek,et al.  Process parameters for the high-scale production of alginate-encapsulated stem cells for storage and distribution throughout the cell therapy supply chain , 2017 .

[37]  Margarida Serra,et al.  Expansion of 3D human induced pluripotent stem cell aggregates in bioreactors: Bioprocess intensification and scaling-up approaches. , 2017, Journal of biotechnology.

[38]  David V. Schaffer,et al.  Expansion of human pluripotent stem cells , 2017 .

[39]  Karl-Heinz Krause,et al.  A 3D printed microfluidic device for production of functionalized hydrogel microcapsules for culture and differentiation of human Neuronal Stem Cells (hNSC). , 2016, Lab on a chip.

[40]  Eduardo Moreno,et al.  Tissue Crowding Induces Caspase-Dependent Competition for Space , 2016, Current Biology.

[41]  Y. Shao,et al.  Lumen Formation Is an Intrinsic Property of Isolated Human Pluripotent Stem Cells , 2015, Stem cell reports.

[42]  David J. Mooney,et al.  Regenerative medicine: Current therapies and future directions , 2015, Proceedings of the National Academy of Sciences.

[43]  Jing Zhou,et al.  Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids , 2015, Nature Communications.

[44]  D. Melton,et al.  An improved ScoreCard to assess the differentiation potential of human pluripotent stem cells , 2015, Nature Biotechnology.

[45]  K. Garber.  RIKEN suspends first clinical trial involving induced pluripotent stem cells , 2015, Nature Biotechnology.

[46]  Y. Sakai,et al.  Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle. , 2015, Journal of visualized experiments : JoVE.

[47]  Guojun Sheng.  Epiblast morphogenesis before gastrulation. , 2015, Developmental biology.

[48]  E. Tzanakakis,et al.  Increased culture density is linked to decelerated proliferation, prolonged G1 phase, and enhanced propensity for differentiation of self-renewing human pluripotent stem cells. , 2015, Stem cells and development.

[49]  E. P. Furlani,et al.  Analysis of stem cell culture performance in a microcarrier bioreactor system , 2014 .

[50]  Daniel Coca,et al.  Time-Lapse Analysis of Human Embryonic Stem Cells Reveals Multiple Bottlenecks Restricting Colony Formation and Their Relief upon Culture Adaptation , 2014, Stem cell reports.

[51]  J. Rowley,et al.  Scalable Passaging of Adherent Human Pluripotent Stem Cells , 2014, PloS one.

[52]  David V. Schaffer,et al.  A fully defined and scalable 3D culture system for human pluripotent stem cell expansion and differentiation , 2013, Proceedings of the National Academy of Sciences.

[53]  Nicolas Bremond,et al.  Cellular capsules as a tool for multicellular spheroid production and for investigating the mechanics of tumor progression in vitro , 2013, Proceedings of the National Academy of Sciences.

[54]  G. Weir,et al.  Core–Shell Hydrogel Microcapsules for Improved Islets Encapsulation , 2013, Advanced healthcare materials.

[55]  Navid B. Saleh,et al.  A Novel Core-Shell Microcapsule for Encapsulation and 3D Culture of Embryonic Stem Cells. , 2013, Journal of materials chemistry. B.

[56]  Akira Niwa,et al.  Morphologic and Gene Expression Criteria for Identifying Human Induced Pluripotent Stem Cells , 2012, PloS one.

[57]  J. Nichols,et al.  Pluripotency in the embryo and in culture. , 2012, Cold Spring Harbor perspectives in biology.

[58]  R. Rao,et al.  Analysis of Embryoid Bodies Derived from Human Induced Pluripotent Stem Cells as a Means to Assess Pluripotency , 2012, Stem cells international.

[59]  P. Alves,et al.  Microencapsulation Technology: A Powerful Tool for Integrating Expansion and Cryopreservation of Human Embryonic Stem Cells , 2011, PloS one.

[60]  Robert J. Thomas,et al.  Expansion of human mesenchymal stem cells on microcarriers , 2011, Biotechnology Letters.

[61]  Todd C McDevitt,et al.  The multiparametric effects of hydrodynamic environments on stem cell culture. , 2011, Tissue engineering. Part B, Reviews.

[62]  Michael J. Ziller,et al.  Reference Maps of Human ES and iPS Cell Variation Enable High-Throughput Characterization of Pluripotent Cell Lines , 2011, Cell.

[63]  D. Kehoe,et al.  Scalable stirred-suspension bioreactor culture of human pluripotent stem cells. , 2010, Tissue engineering. Part A.

[64]  M. Simon,et al.  The role of oxygen availability in embryonic development and stem cell function , 2008, Nature Reviews Molecular Cell Biology.

[65]  D. Scadden,et al.  Deconstructing stem cell self-renewal: genetic insights into cell-cycle regulation , 2008, Nature Reviews Genetics.

[66]  T. Ichisaka,et al.  Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors , 2007, Cell.

[67]  J. Thomson,et al.  Derivation of human embryonic stem cells in defined conditions , 2006, Nature Biotechnology.

[68]  E. Maltepe,et al.  Oxygen, epigenetics and stem cell fate. , 2006, Regenerative medicine.

[69]  R. Roberts,et al.  Low O2 tensions and the prevention of differentiation of hES cells. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[70]  P. Zandstra,et al.  Scalable production of embryonic stem cell-derived cells. , 2005, Methods in molecular biology.

[71]  J. Itskovitz‐Eldor,et al.  Controlled, Scalable Embryonic Stem Cell Differentiation Culture , 2004, Stem cells.

[72]  J. Thomson,et al.  Embryonic stem cell lines derived from human blastocysts. , 1998, Science.

[73]  D. I. Wang,et al.  Effects of microcarrier concentration in animal cell culture , 1988, Biotechnology and bioengineering.

[74]  S. Tomotika.  On the Instability of a Cylindrical Thread of a Viscous Liquid Surrounded by Another Viscous Fluid , 1935 .