Interleukin-2- and interferon-gamma-secreting T cells in normal and diseased human intestinal mucosa.

A sensitive reverse haemolytic plaque assay to detect lymphokine-secreting T cells, and Northern blot analysis to detect expression of lymphokine messenger RNA (mRNA) were used to study interferon-gamma (IFN-gamma) and interleukin-2 (IL-2) production in the mucosa of children with Crohn's disease or ulcerative colitis (UC), and in histologically normal mucosa from patients without inflammatory bowel disease. In the mucosa of most patients with UC and control patients, IL-2- and IFN-gamma-secreting cells were absent or were present at only low levels. In contrast, in mucosa from patients with Crohn's disease, lymphokine-secreting cells were readily detectable (3-18%). IFN-gamma mRNA was detected by Northern blot analysis in 5/6 Crohn's tissues, but only in 1/5 UC samples and none of nine samples of control mucosa. These studies reveal an ongoing cell-mediated immune response in the mucosa in Crohn's disease.

[1]  A. Cooke,et al.  Tumour necrosis factor‐alpha and interferon‐gamma production measured at the single cell level in normal and inflamed human intestine , 1990, Clinical and experimental immunology.

[2]  T. Macdonald,et al.  Differential expression of CD25 (interleukin-2 receptor) on lamina propria T cells and macrophages in the intestinal lesions in Crohn's disease and ulcerative colitis. , 1990, Gut.

[3]  J. D. Neill,et al.  Detection of hormone release from individual cells in mixed populations using a reverse hemolytic plaque assay. , 1983, Endocrinology.

[4]  J. Spencer,et al.  Selective biopsy of human Peyer's patches during ileal endoscopy. , 1987, Gastroenterology.

[5]  A. Ferguson Why study T cell subsets in Crohn's disease? , 1983, Gut.

[6]  J. Brynskov,et al.  Increased concentrations of interleukin 1 beta, interleukin-2, and soluble interleukin-2 receptors in endoscopical mucosal biopsy specimens with active inflammatory bowel disease. , 1992, Gut.

[7]  T. Bayless,et al.  Increased interleukin-2 messenger RNA in the intestinal mucosal lesions of Crohn's disease but not ulcerative colitis. , 1992, Gastroenterology.

[8]  C. Lewis,et al.  Heterogeneity among human mononuclear phagocytes in their secretion of lysozyme, interleukin 1 and type‐β transforming growth factor: a quantitative analysis at the single‐cell level , 1989, European journal of immunology.

[9]  W. Rutter,et al.  Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. , 1979, Biochemistry.

[10]  U. Andersson,et al.  Simultaneous production of interleukin 2, interleukin 4 and interferon‐γ by activated human blood lymphocytes , 1990, European journal of immunology.

[11]  C. Fiocchi,et al.  Intestinal immune reactivity to interleukin 2 differs among Crohn's disease, ulcerative colitis, and controls. , 1989, Gastroenterology.

[12]  P. Beverley,et al.  Functional subsets of T cells defined by isoforms of CD45. , 1992, Biochemical Society transactions.

[13]  M. Capobianchi,et al.  Spontaneous release of interferon gamma by intestinal lamina propria lymphocytes in Crohn's disease. Kinetics of in vitro response to interferon gamma inducers. , 1991, Gut.

[14]  R. Miller,et al.  Estimation by limiting dilution analysis of human IL 2-secreting T cells: detection of IL 2 produced by single lymphokine-secreting T cells. , 1986, Journal of immunology.

[15]  R. Hopkins,et al.  Spontaneous release of a factor with properties of T cell growth factor from a continuous line of primate tumor T cells. , 1981, Journal of immunology.

[16]  C. Fiocchi,et al.  Loss of interleukin-2-producing intestinal CD4+ T cells in inflammatory bowel disease. , 1991, Gastroenterology.

[17]  T. Macdonald,et al.  The role of activated T lymphocytes in gastrointestinal disease , 1990, Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology.

[18]  P. Krajči,et al.  Immunobiology and immunopathology of human gut mucosa: humoral immunity and intraepithelial lymphocytes. , 1989, Gastroenterology.

[19]  B. Morson,et al.  Crohn's Disease (Regional Enteritis) of the Large Intestine and its Distinction from Ulcerative Colitis , 1960, Gut.

[20]  R. Coffman,et al.  Heterogeneity of cytokine secretion patterns and functions of helper T cells. , 1989, Advances in immunology.

[21]  P. S. Richards,et al.  Measurement of cytokine release by human cells. A quantitative analysis at the single cell level using the reverse haemolytic plaque assay. , 1990, Journal of immunological methods.