Optimization of biotechnological parameters and characterization of catalytic properties of thermostable polygalacturonase from a chromium tolerant strain of Trichoderma pseudokoningii.

The hexavalent chromium tolerant strain of Trichoderma pseudokoningii, isolated from chromium enriched tannery effluent was found to produce extracellular polygal acturonase even in presence of chromium. Although there was a negative correlation between the amount of hexavale nt chromium present in the cultivation medium and a mount of polygalacturonase produced, moderate amount (300 U/ml) of enzymes was found to be produced even in presence of high amount of hexavalent chromium (0.80 gm/ml) in medium. The strain was found to synthesise maximum level of polygalacturonase when the culture medium was supplemented with 1.5 % (w/v) pure pectin as sole carbon source at pH 7.0 and 37 o C. The highest level of extracellular enzyme synthe sis could be achieved at 72 nd hour of growth. The enzyme was stable under a wide range of pH (5.0-8.0) and up to 80 % of enzymatic activity was retained even after exposure to 90 o C for 30 minutes.

[1]  M. Arif,et al.  Polygalacturonase production from Rhizomucor pusillus isolated from fruit markets of Uttar Pradesh , 2013 .

[2]  V. Pande,et al.  Production, Purification, and Characterization of Polygalacturonase from Rhizomucor pusillus Isolated from Decomposting Orange Peels , 2012, Enzyme research.

[3]  Kalaichelvan Pt,et al.  Production and Optimization of Pectinase from Bacillus sp. MFW7 using Cassava Waste , 2012 .

[4]  Dharmendra Singh,et al.  TOLERANCE AND BIODERADATION CAPACITY OF Trichoderma viride WITH SPECIAL REFERENCE TO HEAVY METALS (Cr, Cd) , 2012 .

[5]  Rina Rani Ray,et al.  Current Commercial Perspective of Rhizopus oryzae: A Review , 2011 .

[6]  D. Arotupin,et al.  Effect of carbon and nitrogen sources on polygalacturonase production by Trichoderma viride (BITRS-1001) isolated from tar sand in Ondo State, Nigeria , 2011 .

[7]  D. Kalyani,et al.  Textile dye degradation by bacterial consortium and subsequent toxicological analysis of dye and dye metabolites using cytotoxicity, genotoxicity and oxidative stress studies. , 2011, Journal of hazardous materials.

[8]  R. Ray,et al.  Extracellular Thermostable Polygalacturonase from Bacillus sp. AD 1 , 2011 .

[9]  K. Mukherjea,et al.  Uptake and removal of toxic Cr(VI) by Pseudomonas aeruginosa: physico-chemical and biological evaluation , 2011 .

[10]  Humberto González-Díaz,et al.  Alignment-free prediction of polygalacturonases with pseudofolding topological indices: experimental isolation from Coffea arabica and prediction of a new sequence. , 2009, Journal of proteome research.

[11]  Eleni Gomes,et al.  Pectin and Pectinases: Production, Characterization and Industrial Application of Microbial Pectinolytic Enzymes , 2009 .

[12]  T. Kumosani,et al.  Characterization of a Polygalacturonase from Trichoderma harzianum Grown on Citrus Peel with Application for Apple Juice , 2009 .

[13]  Omojasola Pf,et al.  Cellulase production by Trichoderma longi, Aspergillus niger and Saccharomyces cerevisae cultured on waste materials from orange. , 2008 .

[14]  P. F. Omojasola,et al.  Cellulase production by Trichoderma longi, Aspergillus niger and Saccharomyces cerevisae cultured on waste materials from orange. , 2008, Pakistan journal of biological sciences : PJBS.

[15]  E. Cristiani-Urbina,et al.  Hexavalent Chromium Removal by a Trichoderma inhamatum Fungal Strain Isolated from Tannery Effluent , 2007 .

[16]  S. A. Mohamed,et al.  Biochemical characterization of an extracellular polygalacturonase from Trichoderma harzianum. , 2006, Journal of biotechnology.

[17]  E. Gomes,et al.  PRODUCTION AND PARTIAL CHARACTERIZATION OF POLYGALACTURONASES PRODUCED BY THERMOPHILIC MONASCUS SP N8 AND BY THERMOTOLERANT ASPERGILLUS SP N12 ON SOLID-STATE FERMENTATION , 2006 .

[18]  E. Gomes,et al.  PRODUCTION AND PARTIAL CHARACTERIZATION OF POLYGALACTURONASES PRODUCED BY THERMOPHILIC MONASCUS SP N 8 AND BY THERMOTOLERANT ASPERGILLUS SP N 12 ON SOLID-STATE FERMENTATION , 2006 .

[19]  W. Apel,et al.  Low temperature reduction of hexavalent chromium by a microbial enrichment consortium and a novel strain of Arthrobacter aurescens , 2006, BMC Microbiology.

[20]  Reena Gupta,et al.  Microbial pectinolytic enzymes: A review , 2005 .

[21]  U. Phutela,et al.  Pectinase and polygalacturonase production by a thermophilic Aspergillus fumigatus isolated from decomposting orange peels , 2005 .

[22]  A. U. Khan,et al.  Production and regulation of lignin degrading enzymes from Lentinus squarrosulus (mont.) Singer and Psathyrella atroumbonata Pegler , 2003 .

[23]  J. Mikkelsen,et al.  New polygalacturonases from Trichoderma reesei: characterization and their specificities to partially methylated and acetylated pectins. , 2003, Carbohydrate research.

[24]  E. Gomes,et al.  Pectinolytic enzyme production by Bacillus species and their potential application on juice extraction , 2001 .

[25]  Ricard,et al.  Pectinase in papermaking: solving retention problems in mechanical pulps bleached with hydrogen peroxide. , 2000, Enzyme and microbial technology.

[26]  P. Rodis,et al.  Enhanced polygalacturonase production by Aspergillus niger NRRL‐364 grown on supplemented citrus pectin , 1993 .

[27]  I. Labudová,et al.  Purification and characterization of pectinesterase and polygalacturonase from Trichoderma reesei , 1985 .

[28]  P. Bernfeld,et al.  [17] Amylases, α and β , 1955 .