Biochemical changes in cultivars of sweet oranges infected with citrus tristeza virus.

Citrus fruit production occupies a place of considerable importance in the economy of the world including Pakistan. Tristeza disease caused by Citrus Tristeza Virus (CTV) exists in various forms that may or may not cause symptoms in the plants. The bioactive compounds and antioxidants are naturally present in plants and provide a defense mechanism that is generally accelerated in response to a stress. The objective of the present study was to target and analyze the citrus plants that were CTV positive to observe the changes in the enzymatic and non-enzymatic antioxidants of citrus (Sweet Oranges only). It was observed that in response to CTV infection, both the non-enzymatic antioxidants (total flavonoid, ascorbic acid, phenolic acid) and enzymatic antioxidants (catalase, superoxide dismutase and peroxidase) activities showed an increasing trend overall. The profiling of antioxidants in response to a viral infection may help in the discovery of new biomarkers that can be used as a monitoring tool in disease management.

[1]  C. Pirovani,et al.  Citrus tristeza virus (CTV) Causing Proteomic and Enzymatic Changes in Sweet Orange Variety “Westin” , 2015, PloS one.

[2]  C. Lau,et al.  Antioxidant and antiangiogenic properties of phenolic extract from Pleurotus tuber-regium. , 2014, Journal of agricultural and food chemistry.

[3]  Changyong Zhou,et al.  Distribution and Research Advances of Citrus tristeza virus , 2012 .

[4]  H. Mohamed,et al.  Impact of secondary metabolites and related enzymes in flax resistance and/or susceptibility to powdery mildew , 2012 .

[5]  Changyong Zhou,et al.  INFLUENCE OF THE QUANTITY AND VARIABILITY OF CITRUS TRISTEZA VIRUS ON TRANSMISSIBILITY BY SINGLE TOXOPTERA CITRICIDA , 2011 .

[6]  Jiyong Park,et al.  Optimization of supercritical fluid extraction of bioactive compounds from grape (Vitis labrusca B.) peel by using response surface methodology. , 2010 .

[7]  R. Tiwari,et al.  Techniques for evaluation of medicinal plant products as antimicrobial agents: Current methods and future trends , 2010 .

[8]  C. Abdelly,et al.  Effects of salt on lipid peroxidation and antioxidant enzyme activities of Catharanthus roseus suspension cells , 2005 .

[9]  R. Mittler,et al.  Reactive oxygen gene network of plants. , 2004, Trends in plant science.

[10]  I. Macháčková,et al.  Characterization of antioxidant compounds in Jaffa sweeties and white grapefruits , 2004 .

[11]  S. Murphy,et al.  Vitamin C and flavonoid levels of fruits and vegetables consumed in Hawaii , 2004 .

[12]  J. A. Navas‐Cortés,et al.  Induction of an antioxidant enzyme system and other oxidative stress markers associated with compatible and incompatible interactions between chickpea (Cicer arietinum L.) and Fusarium oxysporum f. sp.ciceris , 2002 .

[13]  L. Suntornsuk,et al.  Quantitation of vitamin C content in herbal juice using direct titration. , 2002, Journal of pharmaceutical and biomedical analysis.

[14]  L. Navarro,et al.  Incidence and epidemiology of Citrus tristeza virus in the Valencian community of Spain. , 2000, Virus research.

[15]  M. D. Onghia Occurrence and spread of Citrus Tristeza in the Mediterranean area K . Djelouah and A . , 2013 .

[16]  P. Kefalas,et al.  Radical scavenging activity of various extracts and fractions of sweet orange peel (Citrus sinensis) , 2006 .

[17]  V. Lattanzio,et al.  Role of phenolics in the resistance mechanisms of plants against fungal pathogens and insects. , 2006 .

[18]  E. W. Orlandi,et al.  Active oxygen in plant pathogenesis. , 1995, Annual review of phytopathology.