The effects of beta-2'-deoxythioguanosine on survival and progression in mammalian cells.

Summary Synchronized Chinese hamster ovary cells were most sensitive to β-2′-deoxythioguanosine (β-TGdR) in early and mid S phase. However, little effect on DNA synthesis was observed. One possible mechanism that could account for cell lethality in S-phase cells would be errors made in DNA replication subsequent to incorporation of β-TGdR into DNA. Mitotic cells were totally unaffected and G 1 cells were only slightly sensitive to β-TGdR. Treatment with β-TGdR did not cause progression or division delay, even when cells were treated in early and mid S phase when survival was reduced to 11%.