Evaluation of in vitro and in vivo biocompatibility of a myo-inositol hexakisphosphate gelated polyaniline hydrogel in a rat model

Recent advances in understanding the interaction between electricity and cells/biomolecules have generated great interest in developing biocompatible electrically conductive materials. In this study, we investigated the biocompatibility of a myo-inositol hexakisphosphate gelated polyaniline hydrogel using in vitro and in vivo experiments in a rat model. The polyaniline hydrogel was used to coat a polycaprolactone scaffold and was cultured with rat endothelial progenitor cells differentiated from rat adipose-derived stem cells. Compared with the control sample on a pristine polycaprolactone scaffold, the treated polyaniline hydrogel had the same non-poisonous/cytotoxicity grade, enhanced cell adhesion, and a higher cell proliferation/growth rate. In implant studies, the polyaniline hydrogel sample induced milder inflammatory responses than did the control at the same time points. Combining the advantages of a biocompatible hydrogel and an organic conductor, the inositol phosphate-gelated polyaniline hydrogel could be used in bioelectronics applications such as biosensors, neural probes, cell stimulators, medical electrodes, tissue engineering, and electro-controlled drug delivery.

[1]  Sook Hee Ku,et al.  Synergic effects of nanofiber alignment and electroactivity on myoblast differentiation. , 2012, Biomaterials.

[2]  G. Crabtree,et al.  Inositol Phosphates in the Nucleus , 2000, Science.

[3]  Jaroslav Stejskal,et al.  Biocompatibility of polyaniline , 2012 .

[4]  Kevin A. Rocco,et al.  In vivo applications of electrospun tissue-engineered vascular grafts: a review. , 2014, Tissue engineering. Part B, Reviews.

[5]  P. Hawkins,et al.  Inhibition of iron-catalysed hydroxyl radical formation by inositol polyphosphates: a possible physiological function for myo-inositol hexakisphosphate. , 1993, The Biochemical journal.

[6]  Anthony Guiseppi-Elie,et al.  Electroconductive hydrogels: synthesis, characterization and biomedical applications. , 2010, Biomaterials.

[7]  S. Schwimmer,et al.  Phytic Acid as a Phosphorus Reservoir in the Developing Potato Tuber , 1962, Nature.

[8]  Jun Li,et al.  Surface coating with a thermoresponsive copolymer for the culture and non-enzymatic recovery of mouse embryonic stem cells. , 2009, Macromolecular bioscience.

[9]  J. Travas-sejdic,et al.  Functional polyaniline nanofibre mats for human adipose-derived stem cell proliferation and adhesion , 2013 .

[10]  A. Shamsuddin.  Metabolism and cellular functions of IP6: a review. , 1999, Anticancer research.

[11]  M. Hanley,et al.  Metabolism and biological activities of inositol pentakisphosphate and inositol hexakisphosphate. , 1995, Biochemical pharmacology.

[12]  Christine E. Schmidt,et al.  Conducting polymers in biomedical engineering , 2007 .

[13]  C T Laurencin,et al.  Preliminary in vivo report on the osteocompatibility of poly(anhydride-co-imides) evaluated in a tibial model. , 1998, Journal of biomedical materials research.

[14]  Bianxiao Cui,et al.  Nanowire transistors: room for manoeuvre. , 2014, Nature nanotechnology.

[15]  B D Malhotra,et al.  Recent advances in polyaniline based biosensors. , 2011, Biosensors & bioelectronics.

[16]  A. Boccaccini,et al.  Tissue engineering of electrically responsive tissues using polyaniline based polymers: a review. , 2014, Biomaterials.

[17]  Zhenan Bao,et al.  Hierarchical nanostructured conducting polymer hydrogel with high electrochemical activity , 2012, Proceedings of the National Academy of Sciences.

[18]  Kevin A. Rocco,et al.  In vivo applications of electrospun tissue-engineered vascular grafts: a review. , 2014, Tissue engineering. Part B, Reviews.

[19]  L. Poole-Warren,et al.  Conducting polymer-hydrogels for medical electrode applications , 2010, Science and technology of advanced materials.

[20]  Yi Shi,et al.  A nanostructured conductive hydrogels-based biosensor platform for human metabolite detection. , 2015, Nano letters.

[21]  R. Gurny,et al.  Long term performance of polycaprolactone vascular grafts in a rat abdominal aorta replacement model. , 2012, Biomaterials.

[22]  S. Shears,et al.  Assessing the omnipotence of inositol hexakisphosphate. , 2001, Cellular signalling.

[23]  David C. Martin,et al.  Polymerization of the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) around living neural cells. , 2007, Biomaterials.

[24]  Lijia Pan,et al.  Rational design and applications of conducting polymer hydrogels as electrochemical biosensors. , 2015, Journal of materials chemistry. B.

[25]  Xin Zhang,et al.  Histone deacetylase inhibitor sodium butyrate promotes the osteogenic differentiation of rat adipose‐derived stem cells , 2014, Development, growth & differentiation.

[26]  A. Shamsuddin,et al.  IP6: a novel anti-cancer agent. , 1997, Life sciences.

[27]  M. Abidian,et al.  Conducting‐Polymer Nanotubes for Controlled Drug Release , 2006, Advanced materials.

[28]  S E Moulton,et al.  Electrode-Cellular Interface , 2009, Science.

[29]  R. Langer,et al.  Poly(anhydride-co-imides): in vivo biocompatibility in a rat model. , 1998, Biomaterials.

[30]  Z. Li,et al.  Grafting of poly(ethylene oxide) to the surface of polyaniline films through a chlorosulfonation method and the biocompatibility of the modified films. , 2004, Journal of colloid and interface science.

[31]  Matsuhiko Nishizawa,et al.  Conducting polymer electrodes printed on hydrogel. , 2010, Journal of the American Chemical Society.

[32]  Rong Zhang,et al.  Highly sensitive glucose sensor based on pt nanoparticle/polyaniline hydrogel heterostructures. , 2013, ACS nano.

[33]  Colleges.  The Pharmacopoeia of the United States of America , 1894, The Southern Medical Record.

[34]  Robert C. Wolpert,et al.  A Review of the , 1985 .

[35]  J. York,et al.  A phospholipase C-dependent inositol polyphosphate kinase pathway required for efficient messenger RNA export. , 1999, Science.