Renin gene expression in rat tissues: a new quantitative assay method for rat renin mRNA using synthetic cRNA.

A genomic renin exon 9 fragment was subcloned into vector pSPT18 and used for in vitro transcription to obtain 32P-labeled rat renin cRNA. Using this cRNA, we established quantitative solution hybridization and specific Northern blotting assays for renin mRNA. We were able to detect renin mRNA in the kidney, heart ventricle and atrium, brain, testis, and adrenal gland of male rats in the concentrations of 430 +/- 8.1, 110 +/- 1.9, 43 +/- 0.9, 64 +/- 1.1, 47 +/- 0.9 and 11 +/- 0.2 pg mRNA/mg of total RNA, respectively.

[1]  W. Ganong,et al.  Analysis by immunocytochemistry and in situ hybridization of renin and its mRNA in kidney, testis, adrenal, and pituitary of the rat. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[2]  J. Habener,et al.  Angiotensinogen gene is expressed and differentially regulated in multiple tissues of the rat. , 1986, The Journal of clinical investigation.

[3]  J. Ingelfinger,et al.  Identification of renin and angiotensinogen messenger RNA sequences in mouse and rat brains. , 1986, Hypertension.

[4]  T. Tanaka,et al.  Tissue distribution of rat angiotensinogen mRNA and structural analysis of its heterogeneity. , 1986, The Journal of biological chemistry.

[5]  J. Chirgwin,et al.  Cloning and sequence analysis of cDNA for human cathepsin D. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[6]  T. Inagami,et al.  Regional distribution of renin and angiotensinogen in the brain of normotensive (WKY) and spontaneously hypertensive (SHR) rats , 1985, Brain Research.

[7]  D. Melton,et al.  Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. , 1984, Nucleic acids research.

[8]  Y. Doi,et al.  Evidence for an extrarenal source of inactive renin in rats. , 1984, Hypertension.

[9]  R. Kageyama,et al.  Cloning and sequence analysis of cDNA for human renin precursor. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[10]  B. Morris,et al.  Detection of renin mRNA in mouse kidney and submandibular gland by hybridization with renin cDNA. , 1983, Endocrinology.

[11]  Pierre Corvol,et al.  Complete amino acid sequence and maturation of the mouse submaxillary gland renin precursor , 1982, Nature.

[12]  T. Inagami,et al.  Immunohistochemical localization of renin in luteinizing hormone-producing cells of rat pituitary. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[13]  T. Inagami,et al.  Localization of renin in trophoblasts in human chorion laeve at term pregnancy. , 1981, Endocrinology.

[14]  R. Palmiter,et al.  The ovalbumin gene family: Hormonal control of X and Y gene transcription and mRNA accumulation , 1981, Cell.

[15]  C. Auffray,et al.  Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA. , 2005, European journal of biochemistry.

[16]  E. Zimmerman,et al.  Wide distribution of immunoreactive renin in nerve cells of human brain. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[17]  T. Inagami,et al.  Renin in rat and mouse brain: immunohistochemical identification and localization. , 1980, Clinical science.

[18]  S. Hirose,et al.  Specific Antibody to Hog Renal Renin and Its Application to the Direct Radioimmunoassay of Renin in Various Organs , 1979, Circulation research.

[19]  L. Skeggs,et al.  A comparison of the substrate specificities of cathepsin D and pseudorenin. , 1978, The Journal of biological chemistry.

[20]  J. Tang,et al.  Purification and properties of cathepsin D from porcine spleen. , 1976, The Journal of biological chemistry.

[21]  D. Ganten,et al.  Iso-renin of extrarenal origin. "The tissue angiotensinogenase systems". , 1976, The American journal of medicine.

[22]  D. Ganten,et al.  THE ISO‐RENIN ANGIOTENSIN SYSTEMS IN EXTRARENAL TISSUE , 1976, Clinical and experimental pharmacology & physiology.

[23]  T. Inagami,et al.  A radioimmunoassay for the direct measurement of renin in mice and its application to submaxillary gland and kidney studies. , 1974, Endocrinology.

[24]  D. Ganten,et al.  Renin in dog brain. , 1971, The American journal of physiology.