Expression of miR-140-5p and miR-370 in nephroblastoma and its effect on cell proliferation.

PURPOSE This study aimed to explore the expression changes of microRNA (miR)-140-5p and miR-370 in nephroblastoma and its effect on the proliferative ability of nephroblastoma WT_CLS1 and SK-NEP-1 cells. METHODS The expression levels of miR-140-5p and miR-370 was detected by quantitative real-time PCR (qRT-PCR) in cancer tissues and normal adjacent tissues which were collected from 60 patients with nephroblastoma. Expression vectors of miR-140-5p and miR-370 were constructed, and transient transfection of human nephroblastoma cell lines WT_CLS1 and SK-NEP-1 was carried out in vitro. There were three groups of the two genes: Blank cell group (blank group); Gene transfection group (miR-490-5p group, miR-370 group); and No-load transfection group (NLTF group). The proliferative ability of WT_CLS1 and SK-NEP-1 cells was detected by MTT assay. RESULTS The results of miR-140-5p and miR-370 detected by qRT-PCR showed that in cancer tissues the expression level of miR-140-5p was significantly lower than that in adjacent tissues, and the level of miR-370 was significantly higher than that in adjacent tissues, and the difference was statistically significant (p<0.001). The proliferation of WT_CLS1 and SK-NEP-1 cells in miR-140-5p group was significantly lower than that in NLTF group (p<0.05), while the proliferation of WT_CLS1 and SK-NEP-1 cells in miR-370 group was significantly higher than that in NLTF group (p<0.05). CONCLUSION miR-140-5p is lowly expressed and miR-370 is highly expressed in nephroblastoma tissues; miR-370 can promote the proliferation of WT-CLS1 cells in nephroblastoma, and miR-140-5p can inhibit their proliferation and it may become a new target for the treatment of nephroblastoma in the future.

[1]  B. Morel,et al.  Variability in Imaging Practices and Comparative Cumulative Effective Dose for Neuroblastoma and Nephroblastoma Patients at 6 Pediatric Oncology Centers , 2017, Journal of pediatric hematology/oncology.

[2]  Kunihiko Kobayashi,et al.  Extrarenal Nephroblastoma of the Retroperitoneal Space in Children: A Case Report and Review of the Literature. , 2017, Journal of pediatric hematology/oncology.

[3]  N. Sebire,et al.  Whole‐tumor apparent diffusion coefficient measurements in nephroblastoma: Can it identify blastemal predominance? , 2017, Journal of magnetic resonance imaging : JMRI.

[4]  J. Yamate,et al.  Spontaneous nephroblastoma with striated muscle differentiation in an F344 rat , 2017, Journal of toxicologic pathology.

[5]  C. Kahn,et al.  Adipose-Derived Circulating miRNAs Regulate Gene Expression in Other Tissues , 2017, Nature.

[6]  E. Beierle,et al.  The association between nephroblastoma-specific outcomes and high versus low volume treatment centers. , 2017, Journal of pediatric surgery.

[7]  C. Cafferata,et al.  Outcome of Nephroblastoma Treatment According to the SIOP-2001 Strategy at a Single Institution in Argentina , 2017, Journal of pediatric hematology/oncology.

[8]  Min Liu,et al.  Long non-coding RNA Unigene56159 promotes epithelial-mesenchymal transition by acting as a ceRNA of miR-140-5p in hepatocellular carcinoma cells. , 2016, Cancer letters.

[9]  H. Alatassi,et al.  Nephroblastoma Arising from Primary Testicular Germ Cell Tumor: A Case Report and Literature Review , 2016, Case reports in pathology.

[10]  H. Kidoya,et al.  Regulation of SLD5 gene expression by miR-370 during acute growth of cancer cells , 2016, Scientific Reports.

[11]  J. Au,et al.  A Case of Bilateral Cystic Partially Differentiated Nephroblastoma vs Cystic Wilms' Tumor: Highlighting a Diagnostic Dilemma. , 2016, Urology.

[12]  Andreas Trumpp,et al.  Circulating miRNAs with prognostic value in metastatic breast cancer and for early detection of metastasis. , 2016, Carcinogenesis.

[13]  O. Zakaria,et al.  Efficacy of Different Protocols in Treatment of Nephroblastoma: A revisit. , 2016, The Gulf journal of oncology.

[14]  F. Aqil,et al.  Exosomal miRNAs as biomarkers of recurrent lung cancer , 2016, Tumor Biology.

[15]  J. Yakisich,et al.  Role of apoptosis-related miRNAs in resveratrol-induced breast cancer cell death , 2016, Cell Death & Disease.

[16]  Clive Wilson,et al.  Exosomal miRNAs as cancer biomarkers and therapeutic targets , 2016, Journal of extracellular vesicles.

[17]  Yan Li,et al.  Physcion induces apoptosis in hepatocellular carcinoma by modulating miR-370. , 2016, American journal of cancer research.

[18]  L. Tuyisenge,et al.  Treating childhood cancer in Rwanda: the nephroblastoma example , 2015, The Pan African medical journal.

[19]  S. Teerthanath.  Extrarenal Nephroblastoma , 2011, Journal of laboratory physicians.

[20]  A. Sauvanet,et al.  Pancreatic metastasis from nephroblastoma: an unusual entity. , 2009, JOP : Journal of the pancreas.

[21]  S. Ekenze,et al.  The challenge of nephroblastoma in a developing country. , 2006, Annals of oncology : official journal of the European Society for Medical Oncology.

[22]  S. Singh,et al.  Cystic partially differentiated nephroblastoma: a rare differentiated variant of Wilm's tumour. , 2006, Journal of postgraduate medicine.

[23]  N. Ito.  EXPERIMENTAL STUDIES ON TUMORS OF THE URINARY SYSTEM OF RATS INDUCED BY CHEMICAL CARCINOGENS * , 1973, Acta pathologica japonica.