Novel application of a whole blood intracellular cytokine detection assay to quantitate specific T-cell frequency in field studies.

[1]  Holden T Maecker,et al.  Performance of plate-based cytokine flow cytometry with automated data analysis , 2003, BMC Immunology.

[2]  J. Blackwell,et al.  BCG-induced increase in interferon-gamma response to mycobacterial antigens and efficacy of BCG vaccination in Malawi and the UK: two randomised controlled studies , 2002, The Lancet.

[3]  M. Atkins,et al.  Immunologic Monitoring of Cancer Vaccine Therapy: Results of a Workshop Sponsored by the Society for Biological Therapy , 2002, Journal of immunotherapy.

[4]  E. Rosenberg,et al.  Functionally Inert HIV-Specific Cytotoxic T Lymphocytes Do Not Play a Major Role in Chronically Infected Adults and Children , 2000, The Journal of experimental medicine.

[5]  V. Maino,et al.  Immunofluorescence Analysis of T-Cell Responses in Health and Disease , 2000, Journal of Clinical Immunology.

[6]  H. Maecker,et al.  Optimization of whole blood antigen-specific cytokine assays for CD4(+) T cells. , 2000, Cytometry.

[7]  V. Maino,et al.  Normal human CD4+ memory T cells display broad heterogeneity in their activation threshold for cytokine synthesis. , 1998, Journal of immunology.

[8]  V. Maino,et al.  Identification of functional subsets by flow cytometry: intracellular detection of cytokine expression. , 1998, Cytometry.

[9]  C. Pitcher,et al.  Determination of antigen-specific memory/effector CD4+ T cell frequencies by flow cytometry: evidence for a novel, antigen-specific homeostatic mechanism in HIV-associated immunodeficiency. , 1997, The Journal of clinical investigation.

[10]  Philip J. R. Goulder,et al.  Phenotypic Analysis of Antigen-Specific T Lymphocytes , 1996, Science.

[11]  O Ouchterlony,et al.  A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells. , 1983, Journal of immunological methods.

[12]  V. Maino,et al.  Detection of antigen-specific T cell cytokine expression in whole blood by flow cytometry. , 1998, Journal of immunological methods.